Difference between revisions of "Overview"
Jump to navigation
Jump to search
| Line 29: | Line 29: | ||
== Protocols == | == Protocols == | ||
| − | + | <ul> | |
=== Cell culture === | === Cell culture === | ||
| + | <li>[[Blastema_cells|Blastema cells]]</li> | ||
| + | <li>[[Baculovirus_titration|Baculovirus titration]]</li> | ||
| + | <li>[[Myoblasts_electroporation|Myoblasts electroporation]]</li> | ||
| + | <li>[[Adhesive_cell_culture|Adhesive cell culture]]</li> | ||
| + | <li>[[Electroporation_of_blastema_cells|Electroporation of blastema cells]]</li> | ||
| + | <li>[[Electroporation_of_nec|Electroporation of neural epithelia cells (axolotl spinal cord)]]</li> | ||
| + | <li>[[mESC_culture|mESC culture/Cyst formation]]</li> | ||
| + | <li>[[Neurosphere_culture|Neurosphere culture]]</li> | ||
=== Histology === | === Histology === | ||
=== Molecular biology === | === Molecular biology === | ||
| + | </ul> | ||
Revision as of 16:34, 29 November 2012
Introduction
On the pages listed below you can find most common protocols used in the lab as well as a list of lab duties along with the names of the responsible persons. Please, contact the responsible persons if you have any question, suggestions or troubles.
Lab duties
- Antibiotics
- Benzocaine
- Fibronectin
- Gelatine
- Liquid nitrogen
- Primary antibodies
- Secondary antibodies
- Serum immunostaining
- Autoclave
- Cryostat
- Dissecting microscopes
- Leica confocal microscope
- Vacuum pumps
- Waterbath
- Ordering common stuff
- Heat-shock competent cells
Chemicals
Hardware
General
Protocols
- Blastema cells
- Baculovirus titration
- Myoblasts electroporation
- Adhesive cell culture
- Electroporation of blastema cells
- Electroporation of neural epithelia cells (axolotl spinal cord)
- mESC culture/Cyst formation
- Neurosphere culture
Cell culture
Histology
Molecular biology
Booking calendars
Since some pieces of hardware are used intensively it is necessary to book them in advance. The links to the corresponding booking calendars are listed below.
- Fluorescence microscopes
- Microinjectors and electroporators
- Confocal microscopes
- Cryostats