Blastema cells

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Composition of the medium for A1/BL cells

  • 250 ml MEM 21090
  • 100 ml H2O
  • 40 ml FCS for A1 cells
  • 4 ml pen/strep
  • 4 ml insulin
  • 4 ml glutamin

Tip.png NOTE: Filter the medium after mixing!

PBS

Dilute normal PBS (w/o bivalent cations) in water (both are supplied - cupboard in the corridor in opposite of the cell culture).
The final PBS concentration must be 80%.

Trypsin

Dilute 10xTE > in the -20°C freezer in the cell culture preparation room (black labelling) with APBS.