Overview

Introduction

On the pages listed below you can find most common protocols used in the lab as well as a list of lab duties along with the names of the responsible persons. Please, contact the responsible persons if you have any question, suggestions or troubles.

Axolotl

• Maintainance
• Feeding

Lab duties

Chemicals

• Antibiotics
• Benzocaine
• Fibronectin
• Gelatine
• Liquid nitrogen
• Primary antibodies
• Secondary antibodies
• Serum immunostaining

Hardware

• Autoclave
• Cryostat
• Dissecting microscopes
• Leica confocal microscope
• Vacuum pumps
• Waterbath

General

• Ordering common stuff
• Heat-shock competent cells

Protocols

Cell culture

• Blastema cells
• Baculovirus titration
• Myoblasts electroporation
• Adhesive cell culture
• Electroporation of blastema cells
• Electroporation of neural epithelia cells (axolotl spinal cord)
• mESC culture/Cyst formation
• Neurosphere culture

Histology

Molecular biology

Booking calendars

Since some pieces of hardware are used intensively it is necessary to book them in advance. The links to the corresponding booking calendars are listed below.

• Fluorescence microscopes
  • Olympus SZX 16
  • New Olympus SZX 16
  • Olympus OVK
  • Axio Observer
• Microinjectors and electroporators
  • Electroporator
  • 2nd electroporator
  • Microinjector
  • Stereoscope
• Confocal microscopes
  • Leica LSM
• Cryostats
  • Cryostat 1
  • Cryostat 2
• Microtome
  • Microtome

Transgenic lines

• Axolotl (A.mexicanum)
• Frog (X.laevis)

Digital data

Over the years the members of the lab have produced a lot of different data: Sanger sequences, EST libraries, images and so on. Select a category from the list below in order to view or download the data.

Fileservers

Location	Volume size	Description	Access	Location (console only)
/biodata/groups/crtd_tanaka	16 TB	General purpose storage space	Entire Tanaka lab	biocluster: /group/crtd_tanaka
/biodata/groups/tanaka-ests	247 GB	EST sequences	Entire Tanaka lab	biocluster: /group/tanaka-ests
/biodata/groups/axolotl-transcriptome	117 GB	Axolotl transcriptome data	Elly, Sergej	biocluster: /projects/axolotl-transcriptome
/biodata/groups/tanaka-saori	878 GB	Special directory for Saori's data	Elly, Saori, Sergej	biocluster: /group/tanaka-saori

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NOTE: Note that the path is different, when accessing the file server from the console. Usually

Literature

• Theses
• Publications

Databases

• Axolotl EST database contains several ESTs and also some additional information, e.g. the position of the well with the corresponding insert in the Blastema (BL) or Neural tube (NT) library.
• Axologle database contains several different Axolotl transciptome assemblies

Datasets

• Sequences
  • Illumina
  • Sanger
  • Roche/454
• Microarrays

Lab life

• Lab photos
• Further pictures can be found in /biodata/groups/crtd_tanaka/Lab Fun/
  • Heino's farewell
  • Snow axolotl and snowball fight
  • Yu and Saori's farewell
  • Visit of Kathleen/Fabian and Laura/Mathilda
• The recepies for Heino's farewell present can be found under /biodata/groups/crtd_tanaka/Lab Fun/Heino's farewell present/
• The video of the visit of the EU commissioner can be found under /biodata/groups/crtd_tanaka/Lab Fun/