Overview
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Introduction
On the pages listed below you can find most common protocols used in the lab as well as a list of lab duties along with the names of the responsible persons. Please, contact the responsible persons if you have any question, suggestions or troubles.
Axolotl
Lab duties
- Antibiotics
- Benzocaine
- Fibronectin
- Gelatine
- Liquid nitrogen
- Primary antibodies
- Secondary antibodies
- Serum immunostaining
- Autoclave
- Cryostat
- Dissecting microscopes
- Leica confocal microscope
- Vacuum pumps
- Waterbath
- Ordering common stuff
- Heat-shock competent cells
Chemicals
Hardware
General
Protocols
- Blastema cells
- Baculovirus titration
- Myoblasts electroporation
- Adhesive cell culture
- Electroporation of blastema cells
- Electroporation of neural epithelia cells (axolotl spinal cord)
- mESC culture/Cyst formation
- Neurosphere culture
Cell culture
Histology
Molecular biology
Booking calendars
Since some pieces of hardware are used intensively it is necessary to book them in advance. The links to the corresponding booking calendars are listed below.
- Fluorescence microscopes
- Microinjectors and electroporators
- Confocal microscopes
- Cryostats
- Microtome
Transgenic lines
Digital data
Over the years the members of the lab have produced a lot of different data: Sanger sequences, EST libraries, images and so on. Select a category from the list below in order to view or download the data.
Literature
Databases
- Axolotl EST database contains several ESTs and also some additional information, e.g. the position of the well with the corresponding insert in the Blastema (BL) or Neural tube (NT) library.
- Axologle database contains several different Axolotl transciptome assemblies