Overview: Difference between revisions

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Revision as of 13:33, 16 July 2013

Introduction

On the pages listed below you can find most common protocols used in the lab as well as a list of lab duties along with the names of the responsible persons. Please, contact the responsible persons if you have any question, suggestions or troubles.

Axolotl

Maintainance

In this section you will find some general information on how to maintain the animals.

Feeding

This section will give you an overview of how to feed the axolotls.

Interesting observations

In the following section you can find some interesting observations concerning the axolotl, its behavior, caveats of the transgenics and many more.

Lab duties


Protocols

Booking calendars

Since some pieces of hardware are used intensively it is necessary to book them in advance. The links to the corresponding booking calendars are listed below.

Genetics

Transgenic lines

Markers

Marker Full name Target Remarks
DAPI/Hoechst Nuclei Incorporates into the DNA
PCNA Proliferating-Cell-Nuclear-Antigen Dividing cells
Sox2 Preferentially neuronal lineage Progenitor marker for both spinal cord and brain
PAX6 Brain/spinal cord
PAX7 Satellite cells/spinal cord/brain
MHC Myosin heavy chain Muscle fibers
Myf5 Muscle fibers Labels cycling myoblasts
ßIII-tubulin Axons
MBP Myelin basic protein Schwann cells
Prrx1 Blastema/mesodermal cells of limb bud
SMA Blood vessels/endothelial cells
GFAP Glial fibrillary acidic protein Glia
NeuN Differentiated nerve
Leu7 Schwann cells
Xbra Xbrachyury Mesoderm during gastrula stage
Msx1 Undifferentiated cells Transcriptional repressor during embryogenesis
Trypan blue Dead tissues or cells Vital stain
Mef2c Differentiated muscle cells
PH3 Mitotic cells
BrdU Cells in S-phase
Casp3 Dying cells
Gdf5 Joints Joint development marker
Survivin Cleavage furrow
MAP2
SOX9 Cartillage progenitors

Digital data

Over the years the members of the lab have produced a lot of different data: Sanger sequences, EST libraries, images and so on. Select a category from the list below in order to view or download the data.

Fileservers

Location Volume size Description Access Location (console only)
//biodata/groups/crtd_tanaka 16 TB General purpose storage space Entire Tanaka lab biocluster: /group/crtd_tanaka
//biodata/groups/tanaka-ests 247 GB EST sequences Entire Tanaka lab biocluster: /group/tanaka-ests
//biodata/groups/axolotl-transcriptome 500 GB Axolotl transcriptome data Elly, Sergej biocluster: /projects/axolotl-transcriptome
//biodata/groups/tanaka-saori 878 GB Special directory for Saori's data Elly, Saori, Sergej biocluster: /group/tanaka-saori
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NOTE: The path is different, when accessing the file server from the console.

Literature

Databases

  • Axolotl transcriptome website contains the Axolotl transcriptome assemblies
  • Newt transcriptome website contains the Newt transcriptome assembly
  • Red spotted newt transcriptome website contains the red spotted newt transcriptome assembly
  • Axolotl EST database contains several ESTs and also some additional information, e.g. the position of the well with the corresponding insert in the Blastema (BL) or Neural tube (NT) library.
  • Axologle database contains expression profiling data of Axolotl limb regeneration
  • Axolotl BLAST database contains several different BLAST-able Axolotl transciptome assemblies

Datasets

Interesting links

Lab life

  • Lab photos
  • Further pictures can be found in /biodata/groups/crtd_tanaka/LabFun/
  • The recepies for Heino's farewell present can be found under /biodata/groups/crtd_tanaka/LabFun/Heino's farewell present/
  • The video of the visit of the EU commissioner can be found under /biodata/groups/crtd_tanaka/LabFun/
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