Overview: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
|||
| Line 64: | Line 64: | ||
</li> | </li> | ||
<li> | <li> | ||
Microinjectors and electroporators | Microinjectors and electroporators and dissecting microscope | ||
<ul> | <ul> | ||
<li>[http://www.my.calendars.net/electroporator | <li>Room 0.232 | ||
<ul> | |||
<li>[http://www.my.calendars.net/electroporator Nepa Gene]</li> | |||
<li>[http://www.my.calendars.net/ | <li>[http://www.my.calendars.net/electroporator2 ECM 830]</li> | ||
<li>[http://www.my.calendars.net/stereoscope2/d01/11/2012 Olympus dissecting microscope]</li> | |||
</ul> | |||
</li> | |||
<li>Lab | |||
<ul> | |||
<li>[http://www.my.calendars.net/OlympusLab Olympus (next to the axolotls)]</li> | |||
<li>[http://www.my.calendars.net/OlympusLab2 Olympus (next to the Microtome)]</li> | |||
</ul> | |||
</li> | |||
</ul> | </ul> | ||
</li> | </li> | ||
Revision as of 14:01, 20 February 2013
Introduction
On the pages listed below you can find most common protocols used in the lab as well as a list of lab duties along with the names of the responsible persons. Please, contact the responsible persons if you have any question, suggestions or troubles.
Axolotl
Lab duties
- Antibiotics
- Benzocaine
- Fibronectin
- Gelatine
- Liquid nitrogen
- Primary antibodies
- Secondary antibodies
- Serum immunostaining
- Autoclave
- Cryostat
- Dissecting microscopes
- Leica confocal microscope
- Vacuum pumps
- Waterbath
- Ordering common stuff
- Heat-shock competent cells
Chemicals
Hardware
General
Protocols
- Blastema cells
- Baculovirus titration
- Myoblasts electroporation
- Adhesive cell culture
- Electroporation of blastema cells
- Electroporation of neural epithelia cells (axolotl spinal cord)
- mESC culture/Cyst formation
- Neurosphere culture
- Double Immunostaining via antigen retrieval (Sherry)
- Metamorphosis protocol (Sherry)
Cell culture
Histology
Molecular biology
Booking calendars
Since some pieces of hardware are used intensively it is necessary to book them in advance. The links to the corresponding booking calendars are listed below.
- Fluorescence microscopes
- Microinjectors and electroporators and dissecting microscope
- Confocal microscopes
- Cryostats
- Microtome
Transgenic lines
Digital data
Over the years the members of the lab have produced a lot of different data: Sanger sequences, EST libraries, images and so on. Select a category from the list below in order to view or download the data.
Fileservers
| Location | Volume size | Description | Access | Location (console only) |
|---|---|---|---|---|
| //biodata/groups/crtd_tanaka | 16 TB | General purpose storage space | Entire Tanaka lab | biocluster: /group/crtd_tanaka |
| //biodata/groups/tanaka-ests | 247 GB | EST sequences | Entire Tanaka lab | biocluster: /group/tanaka-ests |
| //biodata/groups/axolotl-transcriptome | 117 GB | Axolotl transcriptome data | Elly, Sergej | biocluster: /projects/axolotl-transcriptome |
| //biodata/groups/tanaka-saori | 878 GB | Special directory for Saori's data | Elly, Saori, Sergej | biocluster: /group/tanaka-saori |
Error creating thumbnail: File missing
NOTE: The path is different, when accessing the file server from the console.
Literature
Databases
- Axolotl EST database contains several ESTs and also some additional information, e.g. the position of the well with the corresponding insert in the Blastema (BL) or Neural tube (NT) library.
- Axologle database contains several different Axolotl transciptome assemblies
Datasets
Lab life
- Lab photos
- Further pictures can be found in /biodata/groups/crtd_tanaka/LabFun/
- The recepies for Heino's farewell present can be found under /biodata/groups/crtd_tanaka/LabFun/Heino's farewell present/
- The video of the visit of the EU commissioner can be found under /biodata/groups/crtd_tanaka/LabFun/