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- 08:19, 1 June 2015 diff hist -10 TSA Staining on axolotl-tissue
- 08:18, 1 June 2015 diff hist +10 TSA Staining on axolotl-tissue
- 08:17, 1 June 2015 diff hist -14 TSA Staining on axolotl-tissue
- 08:14, 1 June 2015 diff hist -6 TSA Staining on axolotl-tissue
- 08:12, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue
- 08:06, 1 June 2015 diff hist +9 TSA Staining on axolotl-tissue
- 07:50, 1 June 2015 diff hist +1,096 N TSA Staining on axolotl-tissue Created page with "Solutions: - TNT wash buffer: 0.1M TRIS-HCL, pH 7.5 0.15M NaCl 0.05% Tween20 - TNB buffer: a) for blocking: 0.1M TRIS-HCL, pH 7.5 0.15M NaCl 20% goat serum b) for di..."
- 14:11, 4 May 2015 diff hist -16 MESC culture
- 14:06, 4 May 2015 diff hist 0 MESC culture
- 10:07, 4 May 2015 diff hist -11 MESC culture
- 10:06, 4 May 2015 diff hist -4 MESC culture
- 10:04, 4 May 2015 diff hist +19 MESC culture
- 10:02, 4 May 2015 diff hist +17 MESC culture
- 08:43, 4 May 2015 diff hist +4,160 N MESC culture Created page with "46C-ES cells/cyst formation Thawing of 46C ES cells: Prepare the growth medium: 405 ml DMEM + high glucose * Glutamax (Invitrogen: 31966) 75 ml FCS 5 ml L-Gln 100x (Invitro..."
- 13:20, 17 April 2015 diff hist 0 Electroporation of C2C12 cells
- 13:20, 17 April 2015 diff hist -1 Electroporation of C2C12 cells →material
- 13:17, 17 April 2015 diff hist 0 Electroporation of C2C12 cells →material
- 13:15, 17 April 2015 diff hist +1,228 N Electroporation of C2C12 cells Created page with "== material == Transfection * 1x Steinberger Medium * 1x DMEM without serum - remove the old media - wash the cells with 5 ml PBS - remove the PBS - add 2 ml TE - put the..."
- 12:58, 17 April 2015 diff hist 0 Preparation of Freezing media for A1 cells →Procedure
- 12:54, 17 April 2015 diff hist +11 Preparation of Freezing media for A1 cells
- 12:52, 17 April 2015 diff hist +432 N Preparation of Freezing media for A1 cells Created page with "Materials * DMSO (one ampules from Sigma) * High serum for A1 cells * 2x 50 ml FALCON tubes * 1x 15 ml FALCON tube * Pipettes ** 10x 5 ml FALCON tubes Procedure - clean the a..."
- 12:45, 17 April 2015 diff hist 0 Preparation of Low Serum for A1 cells →Procedure
- 12:44, 17 April 2015 diff hist 0 Preparation of Low Serum for A1 cells →Materials
- 12:40, 17 April 2015 diff hist +261 N Preparation of Low Serum for A1 cells Created page with "== Materials == - 280 ml MEM - 100 ml sterile water - 1 tube Pen/ Strep (4ml) - 1 tube Glutamine (4ml) - 1 tube Insulin (4 ml) - 2 ml FCS - 500 ml filter with bottle == Proce..."
- 10:54, 27 March 2015 diff hist -22 RNA formaldehyde gels current
- 10:52, 27 March 2015 diff hist -3 RNA formaldehyde gels
- 10:51, 27 March 2015 diff hist +13 RNA formaldehyde gels
- 10:48, 27 March 2015 diff hist -17 RNA Extraction from axolotl tissue
- 10:45, 27 March 2015 diff hist -22 Digestion DNA →Digestion current
- 10:44, 27 March 2015 diff hist -28 Digestion DNA
- 10:42, 27 March 2015 diff hist +8 Digestion DNA
- 10:40, 27 March 2015 diff hist +16 Metamorphosis protocol current
- 10:39, 27 March 2015 diff hist +16 Antibody staining current
- 10:36, 27 March 2015 diff hist +11 Labeled AB staining
- 10:33, 27 March 2015 diff hist +4 Labelling Pax6 with Dig-NHS current
- 10:32, 27 March 2015 diff hist -5 Labelling myosin antibody
- 10:31, 27 March 2015 diff hist -18 Purifying IgG1 from Hybridoma Supernatant
- 10:30, 27 March 2015 diff hist -8 Purifying IgG1 from Hybridoma Supernatant
- 10:29, 27 March 2015 diff hist +16 Whole mount ISH on axolotl tissue current
- 10:27, 27 March 2015 diff hist -12 ISH on axolotl tissue sections →Materials
- 10:26, 27 March 2015 diff hist +31 ISH on axolotl tissue sections
- 10:23, 27 March 2015 diff hist +16 Double Immunostaining via antigen retrieval
- 10:22, 27 March 2015 diff hist +16 Alcian Blue Staining current
- 10:00, 27 March 2015 diff hist -23 Hybridoma cells in SFX media
- 09:59, 27 March 2015 diff hist -6 Hybridoma cells in SFX media
- 09:57, 27 March 2015 diff hist -14 Thawing (XB10) hybridoma cells
- 09:54, 27 March 2015 diff hist -14 Preparation of Low Serum for C2C12 cells
- 09:13, 27 March 2015 diff hist 0 C2C12 cell transfection with FuGene →Procedure
- 09:06, 27 March 2015 diff hist +36 C2C12 cell transfection with FuGene
- 09:03, 27 March 2015 diff hist 0 C2C12 cell transfection with FuGene →Procedure
- 09:02, 27 March 2015 diff hist -1 C2C12 cell transfection with FuGene
- 08:57, 27 March 2015 diff hist 0 Myotube preparation of C2C12 cells
- 08:55, 27 March 2015 diff hist 0 Myotube preparation of C2C12 cells
- 08:52, 27 March 2015 diff hist +7 Myotube preparation of C2C12 cells
- 08:50, 27 March 2015 diff hist 0 Passaging C2C12 cells
- 08:48, 27 March 2015 diff hist +1 Preparation of High Serum for A1 cells
- 08:47, 27 March 2015 diff hist 0 BrdU and myosin staining →Materials
- 08:46, 27 March 2015 diff hist +7 BrdU and myosin staining →Materials
- 08:43, 27 March 2015 diff hist 0 Modified myotube prep
- 08:41, 27 March 2015 diff hist 0 Passaging C2C12 cells
- 08:39, 27 March 2015 diff hist -2 Passaging C2C12 cells
- 08:38, 27 March 2015 diff hist 0 Freezing of C2C12 cells
- 08:36, 27 March 2015 diff hist +2 Freezing of C2C12 cells
- 08:35, 27 March 2015 diff hist 0 Thawing frozen C2C12 cells
- 08:34, 27 March 2015 diff hist +2 Thawing frozen C2C12 cells
- 09:58, 25 March 2015 diff hist +16 Electroporation of blastema cells current
- 09:54, 25 March 2015 diff hist +2 Preparation of High Serum for A1 cells
- 09:53, 25 March 2015 diff hist +15 Preparation of High Serum for A1 cells
- 09:52, 25 March 2015 diff hist +11 Preparation of High Serum for A1 cells
- 09:50, 25 March 2015 diff hist 0 BrdU and myosin staining →Staining for BrDU and Myosin:
- 09:49, 25 March 2015 diff hist 0 BrdU and myosin staining →Staining for BrDU and Myosin:
- 09:46, 25 March 2015 diff hist -2 BrdU and myosin staining →Fixing Cells:
- 09:44, 25 March 2015 diff hist +21 BrdU and myosin staining
- 09:42, 25 March 2015 diff hist +3 BrdU and myosin staining
- 09:39, 25 March 2015 diff hist -2 BrdU labelling of A1 cells in 96-well plate
- 09:39, 25 March 2015 diff hist -3 Electroporation of A1 cells
- 09:38, 25 March 2015 diff hist +5 Electroporation of A1 cells
- 09:37, 25 March 2015 diff hist +2 Electroporation of A1 cells
- 09:36, 25 March 2015 diff hist -13 Electroporation of A1 cells
- 09:31, 25 March 2015 diff hist -35 A1 cell passaging myotube
- 09:27, 25 March 2015 diff hist -2 Passaging A1 cell
- 09:27, 25 March 2015 diff hist -4 Freezing of A1 cells
- 09:26, 25 March 2015 diff hist -2 Freezing of A1 cells
- 09:25, 25 March 2015 diff hist 0 Thawing frozen A1 cells
- 09:23, 25 March 2015 diff hist -2 Thawing frozen A1 cells
- 09:22, 25 March 2015 diff hist +4 Electroporation of nec current
- 09:21, 25 March 2015 diff hist +10 Electroporation of nec
- 09:19, 25 March 2015 diff hist -1 Cardiomyocyte Preparation current
- 09:17, 25 March 2015 diff hist -8 G418 medium preparation
- 09:16, 25 March 2015 diff hist -18 G418 medium preparation
- 09:13, 25 March 2015 diff hist -12 Thawing 293FT cells
- 09:11, 25 March 2015 diff hist -2 Myoblasts electroporation
- 09:07, 25 March 2015 diff hist -6 Electroporation of nec
- 09:05, 25 March 2015 diff hist 0 Preparation of Freezing media for C2C12 cells
- 09:05, 25 March 2015 diff hist 0 Preparation of High Serum for C2C12 cells
- 08:58, 25 March 2015 diff hist +10 Preparation of High Serum for C2C12 cells
- 08:55, 25 March 2015 diff hist +597 N Preparation of High Serum for C2C12 cells Created page with "Materials: - 1 tube pen/strep (green, 4 ml) - 1 tube glutamine (blue, 4 ml) → this is not necessary if the DMEM has glutamax - 1 bottle DMEM with 4500 mg/ l glucose - 40 ml..."
- 08:53, 25 March 2015 diff hist +16 Preparation of Freezing media for C2C12 cells
- 08:50, 25 March 2015 diff hist +247 N Preparation of Freezing media for C2C12 cells Created page with "Materials: 25 ml FCS (50%) 20 ml DMEM + Pen/ Strep + Glut (40%) 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro Procedure: - put all stuffs together - filtate the mediu..."
- 12:20, 23 March 2015 diff hist +6 A1 cell transfection with FuGene
- 12:18, 23 March 2015 diff hist +2 A1 cell transfection with FuGene
- 11:36, 23 March 2015 diff hist -11 A1 cell transfection with FuGene
- 11:32, 23 March 2015 diff hist -46 A1 cell transfection with FuGene
- 11:31, 23 March 2015 diff hist +49 A1 cell transfection with FuGene
- 11:30, 23 March 2015 diff hist -1 A1 cell transfection with FuGene
- 11:29, 23 March 2015 diff hist -2 A1 cell transfection with FuGene
- 11:28, 23 March 2015 diff hist -2 A1 cell transfection with FuGene
- 11:27, 23 March 2015 diff hist -1 A1 cell transfection with FuGene
- 11:27, 23 March 2015 diff hist +2 A1 cell transfection with FuGene
- 11:21, 23 March 2015 diff hist +1,463 N A1 cell transfection with FuGene Created page with "== Materials == * Fugene Transfection solution * Complete media for A1 cells * No serum media * ATE * APBS * 10 cm dishes * eppendorf tubes == Procedure == Incubation FuGen..."
- 08:47, 11 March 2015 diff hist +14 Cardiomyocyte Preparation
- 08:46, 11 March 2015 diff hist +1 Cardiomyocyte Preparation
- 08:44, 11 March 2015 diff hist -15 Cardiomyocyte Preparation
- 08:41, 11 March 2015 diff hist +4 Cardiomyocyte Preparation
- 08:40, 11 March 2015 diff hist +12 Cardiomyocyte Preparation
- 08:38, 11 March 2015 diff hist 0 Preparation of Low Serum for C2C12 cells
- 08:36, 11 March 2015 diff hist 0 Thawing 293FT cells
- 08:34, 11 March 2015 diff hist +2 Preparation of Low Serum for C2C12 cells
- 08:32, 11 March 2015 diff hist 0 Preparation of Low Serum for C2C12 cells
- 08:31, 11 March 2015 diff hist -8 Preparation of Low Serum for C2C12 cells
- 08:30, 11 March 2015 diff hist +5 Preparation of Low Serum for C2C12 cells
- 08:30, 11 March 2015 diff hist +475 N Preparation of Low Serum for C2C12 cells Created page with "== materials == <li> 390 mls DMEM-1 tube peni./ strepto. <li> tube glutamine (no insulin) <li> 8 mls Horse serum-1 sterile bottle <li> 500 ml sterile cylinder sterile fi..."
- 08:28, 16 February 2015 diff hist -51 Labelling myosin antibody
- 08:20, 16 February 2015 diff hist -2 Labelling myosin antibody
- 08:18, 16 February 2015 diff hist +20 Labelling myosin antibody
- 08:14, 16 February 2015 diff hist +4 Labelling myosin antibody
- 08:14, 16 February 2015 diff hist +36 Labelling myosin antibody
- 08:10, 16 February 2015 diff hist +978 N Labelling myosin antibody Created page with "0.2 M Borate buffer, pH 8 Biorad protein assay substance IgG standard Anti myosin antibody (4A1025) FlNHS (1.5 mg/ml in DMSO—make up fresh just before use) 100mM glycine, pH..."
- 10:25, 13 February 2015 diff hist -2 Preparation of High Serum for A1 cells
- 10:25, 13 February 2015 diff hist +3 Preparation of High Serum for A1 cells
- 10:24, 13 February 2015 diff hist +5 Preparation of High Serum for A1 cells
- 10:22, 13 February 2015 diff hist -1 Preparation of High Serum for A1 cells
- 10:20, 13 February 2015 diff hist 0 Preparation of High Serum for A1 cells
- 10:19, 13 February 2015 diff hist +7 Preparation of High Serum for A1 cells
- 10:16, 13 February 2015 diff hist +5 Preparation of High Serum for A1 cells
- 10:15, 13 February 2015 diff hist +16 Preparation of High Serum for A1 cells
- 10:09, 13 February 2015 diff hist +4 Preparation of High Serum for A1 cells
- 10:07, 13 February 2015 diff hist +465 N Preparation of High Serum for A1 cells Created page with "For 400 ml FCS 40 ml Eagle’s MEM 252 ml DdH2O 100 ml Pen/ Strep 4 ml Glutamin 4 ml Insulin (stock: 1mg/ ml) 4 ml - grow at 25ºC in 2% CO2 - if you want to gr..."
- 08:40, 13 February 2015 diff hist -14 Alcian Blue Staining
- 08:38, 13 February 2015 diff hist +599 N Alcian Blue Staining Created page with "# Fix sample O/N in 4% PFA freshly made # Wash in PBS, 3X5mins, 3X30mins 3. Wash 20mins in PBST 4. Equilibrate in EtOH, 25, 50%, 10 mins each 5. Place in Alcian blue staini..."
- 08:42, 9 February 2015 diff hist -2 Myoblasts electroporation
- 08:36, 9 February 2015 diff hist +6 Myoblasts electroporation
- 08:35, 9 February 2015 diff hist +4 Myoblasts electroporation
- 08:30, 9 February 2015 diff hist -9 Letter to Froppier (French) current
- 09:29, 2 February 2015 diff hist +265 Letter to Froppier (French)
- 13:00, 12 January 2015 diff hist +16 Freezing of A1 cells
- 12:54, 12 January 2015 diff hist +2 Electroporation of A1 cells
- 12:38, 12 January 2015 diff hist +2 Cloning of C2C12 cells
- 12:37, 12 January 2015 diff hist -7 Cloning of C2C12 cells
- 12:32, 12 January 2015 diff hist +7 Cloning of C2C12 cells
- 12:29, 12 January 2015 diff hist +4 Cloning of C2C12 cells
- 12:28, 12 January 2015 diff hist +2,085 Cloning of C2C12 cells
- 12:24, 12 January 2015 diff hist +1 N Cloning of C2C12 cells Created page with "#"
- 08:40, 12 January 2015 diff hist -1 Freezing of A1 cells
- 08:35, 12 January 2015 diff hist -1 Myoblasts electroporation
- 08:30, 12 January 2015 diff hist +654 N Freezing of A1 cells Created page with "''in one 175 cm2 flask'' Materials: - APBS (100 ml PBS + 25 ml diwater) - TE in APBS (36 ml APBS + 4 ml TE) - 1x 15 ml FALCON tubes - Pipettes - 1 cryo tube - freezing media ..."
- 12:58, 3 November 2014 diff hist -4 C2C12 cell transfection with FuGene
- 12:56, 3 November 2014 diff hist +28 C2C12 cell transfection with FuGene
- 12:52, 3 November 2014 diff hist +2 C2C12 cell transfection with FuGene
- 12:51, 3 November 2014 diff hist 0 C2C12 cell transfection with FuGene
- 12:47, 3 November 2014 diff hist 0 C2C12 cell transfection with FuGene
- 12:43, 3 November 2014 diff hist +1 C2C12 cell transfection with FuGene
- 12:41, 3 November 2014 diff hist -4 C2C12 cell transfection with FuGene
- 12:38, 3 November 2014 diff hist +2 C2C12 cell transfection with FuGene
- 12:36, 3 November 2014 diff hist -42 C2C12 cell transfection with FuGene
- 12:34, 3 November 2014 diff hist +9 C2C12 cell transfection with FuGene
- 12:33, 3 November 2014 diff hist -1 C2C12 cell transfection with FuGene
- 12:31, 3 November 2014 diff hist 0 C2C12 cell transfection with FuGene
- 12:29, 3 November 2014 diff hist +12 C2C12 cell transfection with FuGene
- 12:28, 3 November 2014 diff hist -59 C2C12 cell transfection with FuGene
- 12:19, 3 November 2014 diff hist +3 C2C12 cell transfection with FuGene
- 12:19, 3 November 2014 diff hist +127 C2C12 cell transfection with FuGene
- 12:17, 3 November 2014 diff hist +1 C2C12 cell transfection with FuGene
- 12:16, 3 November 2014 diff hist -1 C2C12 cell transfection with FuGene
- 12:15, 3 November 2014 diff hist -2 C2C12 cell transfection with FuGene
- 12:15, 3 November 2014 diff hist +3 C2C12 cell transfection with FuGene
- 12:14, 3 November 2014 diff hist -1 C2C12 cell transfection with FuGene
- 10:55, 3 November 2014 diff hist +1 C2C12 cell transfection with FuGene
- 10:55, 3 November 2014 diff hist -21 C2C12 cell transfection with FuGene
- 10:52, 3 November 2014 diff hist +7 C2C12 cell transfection with FuGene
- 10:48, 3 November 2014 diff hist +2 C2C12 cell transfection with FuGene
- 10:46, 3 November 2014 diff hist +14 C2C12 cell transfection with FuGene
- 10:35, 3 November 2014 diff hist +131 C2C12 cell transfection with FuGene
- 10:30, 3 November 2014 diff hist +12 C2C12 cell transfection with FuGene
- 10:28, 3 November 2014 diff hist +4 C2C12 cell transfection with FuGene
- 10:25, 3 November 2014 diff hist +3,695 N C2C12 cell transfection with FuGene Created page with "2 x 6cm dishes with C2C12 cells Materials: Day1: 2x 6cm dishes 75 cm2 flask C2C12 cells in a 75 cm2 flask 15 ml FALCON tubes High serum media for C2C12 TE in PBS PBS ..."
- 07:41, 24 October 2014 diff hist 0 Freezing of C2C12 cells
- 07:35, 24 October 2014 diff hist -1 Freezing of C2C12 cells
- 07:35, 24 October 2014 diff hist +8 Freezing of C2C12 cells
- 07:33, 24 October 2014 diff hist -1 Freezing of C2C12 cells
- 07:25, 24 October 2014 diff hist +707 N Freezing of C2C12 cells Created page with "Materials: - 15 ml FALCON tube - PBS w/o Ca2+ and Mg2+ - TE in PBS - Freezing media (10% DMSO + 40% serum + 50% DMEM) - Pipettes and tips - Glas pipetts - cryo tube - ice - f..."
- 10:13, 22 October 2014 diff hist +3 Hybridoma cells clones
- 10:12, 22 October 2014 diff hist -3 Hybridoma cells clones
- 10:10, 22 October 2014 diff hist +2 Hybridoma cells clones →Procedure:
- 09:59, 22 October 2014 diff hist +2 Hybridoma cells clones
- 09:56, 22 October 2014 diff hist -40 Hybridoma cells clones →Procedure:
- 09:55, 22 October 2014 diff hist +38 Hybridoma cells clones →Procedure:
- 09:54, 22 October 2014 diff hist -20 Hybridoma cells clones
- 09:49, 22 October 2014 diff hist -6 Hybridoma cells clones
- 09:45, 22 October 2014 diff hist -7 Hybridoma cells clones
- 09:41, 22 October 2014 diff hist +8 Hybridoma cells clones
- 09:36, 22 October 2014 diff hist +12 Hybridoma cells clones
- 09:35, 22 October 2014 diff hist +5 Hybridoma cells clones
- 09:34, 22 October 2014 diff hist -2 Hybridoma cells clones
- 09:31, 22 October 2014 diff hist -7 Hybridoma cells clones
- 09:30, 22 October 2014 diff hist -24 Hybridoma cells clones
- 09:23, 22 October 2014 diff hist +11,002 N Hybridoma cells clones Created page with "== General execution: == 1) ELISA of the supernatants 2) Antibody staining on cryostat section (7 dpa tail sections) of the positive clones from the ELISA 3) Cloning of a p..."
- 07:44, 20 October 2014 diff hist +115 ISH on axolotl tissue sections
- 07:40, 20 October 2014 diff hist -6 ISH on axolotl tissue sections
- 07:39, 20 October 2014 diff hist -2 ISH on axolotl tissue sections
- 07:38, 20 October 2014 diff hist -4 ISH on axolotl tissue sections
- 07:34, 20 October 2014 diff hist +1 ISH on axolotl tissue sections
- 07:29, 20 October 2014 diff hist +1 ISH on axolotl tissue sections
- 07:26, 20 October 2014 diff hist +12 ISH on axolotl tissue sections
- 11:57, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:57, 13 October 2014 diff hist +15 ISH on axolotl tissue sections
- 11:55, 13 October 2014 diff hist +8 ISH on axolotl tissue sections
- 11:48, 13 October 2014 diff hist +3 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist -7 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist +13 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:46, 13 October 2014 diff hist +18 ISH on axolotl tissue sections
- 11:45, 13 October 2014 diff hist 0 ISH on axolotl tissue sections
- 11:44, 13 October 2014 diff hist -8 ISH on axolotl tissue sections
- 11:42, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:41, 13 October 2014 diff hist -1 ISH on axolotl tissue sections
- 11:40, 13 October 2014 diff hist -5 ISH on axolotl tissue sections
- 11:39, 13 October 2014 diff hist -5 ISH on axolotl tissue sections
- 11:35, 13 October 2014 diff hist +5,086 N ISH on axolotl tissue sections Created page with "== Probe preparation == 1. Mix the following at RT: 10x transcription buffer 2 µl dig Nucleotide mix 2 µl Linearized plasmid x µl (1µg) RNase inhibito..."
- 08:02, 10 October 2014 diff hist +1 Modified myotube prep
- 08:01, 10 October 2014 diff hist +7 Modified myotube prep
- 08:00, 10 October 2014 diff hist +4 Modified myotube prep
- 07:59, 10 October 2014 diff hist +1 Cardiomyocyte Preparation
- 07:58, 10 October 2014 diff hist -3 Cardiomyocyte Preparation
- 07:56, 10 October 2014 diff hist -1 Cardiomyocyte Preparation
- 07:54, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:54, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:53, 10 October 2014 diff hist -2 Cardiomyocyte Preparation
- 07:52, 10 October 2014 diff hist +4 Cardiomyocyte Preparation
- 07:51, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:50, 10 October 2014 diff hist +6 Cardiomyocyte Preparation
- 07:46, 10 October 2014 diff hist -10 Modified myotube prep
- 07:43, 10 October 2014 diff hist -56 Modified myotube prep
- 07:37, 10 October 2014 diff hist 0 Modified myotube prep →Execution
- 07:36, 10 October 2014 diff hist +5 Modified myotube prep
- 07:34, 10 October 2014 diff hist -38 Modified myotube prep
- 07:29, 10 October 2014 diff hist -1 Modified myotube prep
- 07:10, 10 October 2014 diff hist +7 Modified myotube prep
- 07:05, 10 October 2014 diff hist +3,246 N Modified myotube prep Created page with "Induce A1 cells on 10 cm plates to form myotubes by * wash 1x with 5 mls APBS * changing the media to 0.5% serum-AMEM * wait 4 days If desired, myotubes can be purified awa..."
- 08:09, 26 September 2014 diff hist +4 Passaging A1 cell
- 08:07, 26 September 2014 diff hist +6 Passaging A1 cell
- 08:06, 26 September 2014 diff hist +787 Passaging A1 cell
- 07:44, 24 September 2014 diff hist +4 Thawing frozen A1 cells
- 07:43, 24 September 2014 diff hist 0 Thawing frozen A1 cells
- 07:37, 24 September 2014 diff hist +828 N Thawing frozen A1 cells Created page with "== Materials: == - Gelatin (warmed to 37ºC) - HS for A1 cells - 1x 15 ml FALCON tubes - Pipettes - 1x 175 cm2 flasks - frozen cells into liquid nitrogen == Procedure: == - p..."
- 08:45, 5 September 2014 diff hist +2 Electroporation of A1 cells
- 08:45, 5 September 2014 diff hist +15 Electroporation of A1 cells
- 08:43, 5 September 2014 diff hist +11 Electroporation of A1 cells
- 08:41, 5 September 2014 diff hist +14 Electroporation of A1 cells
- 08:36, 5 September 2014 diff hist 0 Electroporation of A1 cells
- 08:35, 5 September 2014 diff hist +7 Electroporation of A1 cells
- 08:16, 5 September 2014 diff hist -3 Electroporation of A1 cells
- 08:14, 5 September 2014 diff hist -4 Electroporation of A1 cells
- 08:12, 5 September 2014 diff hist +100 Electroporation of A1 cells
- 07:26, 5 September 2014 diff hist +3 Electroporation of A1 cells
- 07:24, 5 September 2014 diff hist 0 Electroporation of A1 cells
- 07:23, 5 September 2014 diff hist -5 Electroporation of A1 cells
- 07:22, 5 September 2014 diff hist -13 Electroporation of A1 cells
- 07:20, 5 September 2014 diff hist +12 Electroporation of A1 cells
- 07:19, 5 September 2014 diff hist +4 Electroporation of A1 cells
- 07:18, 5 September 2014 diff hist +899 N Electroporation of A1 cells Created page with "==Materials:== Ice 4 mM cuvettes, N+1 cuvettes Steinberg's, at 4 C. Square pulse electroporator (we use the BTX 830 Squarporator) 1) Put cuvettes on ice, and cool down..."
- 09:33, 3 September 2014 diff hist 0 Thawing frozen C2C12 cells
- 09:31, 3 September 2014 diff hist +711 N Thawing frozen C2C12 cells Created page with "==Materials:== - frozen C2C12 cells - HS for C2C12 cells - pipettes and tips - FALCON tube 15 ml - Cell culture flask ==Procedure:== - heat the HS up to 37ºC - pipette 14 ml..."
- 09:09, 3 September 2014 diff hist 0 Myotube preparation of C2C12 cells
- 09:05, 3 September 2014 diff hist 0 Myotube preparation of C2C12 cells
- 09:03, 3 September 2014 diff hist +8 Myotube preparation of C2C12 cells
- 09:02, 3 September 2014 diff hist +2,773 N Myotube preparation of C2C12 cells Created page with "Materials for 1 x 96 well plate: - DMEM (3.5 ml) –purely - Fibronectin (final diluted concentration should be 20 ng/µl)) - 1x 96 well plate - 2% Horse Serum – Low Serum (..."
- 07:17, 29 August 2014 diff hist -6 Whole mount ISH on axolotl tissue
- 07:15, 29 August 2014 diff hist -24 Whole mount ISH on axolotl tissue
- 07:14, 29 August 2014 diff hist +1 Whole mount ISH on axolotl tissue
- 07:14, 29 August 2014 diff hist +3 Whole mount ISH on axolotl tissue
- 07:13, 29 August 2014 diff hist +1 Whole mount ISH on axolotl tissue
- 07:12, 29 August 2014 diff hist +19 Whole mount ISH on axolotl tissue
- 07:08, 29 August 2014 diff hist +3 Whole mount ISH on axolotl tissue
- 07:06, 29 August 2014 diff hist +6 Whole mount ISH on axolotl tissue
- 07:04, 29 August 2014 diff hist -40 Whole mount ISH on axolotl tissue
- 07:00, 29 August 2014 diff hist +3,046 N Whole mount ISH on axolotl tissue Created page with "# fix embryo or tail blastema in MEMFA (0.1M MOPS pH7.4, 2mM EGTA, 1mM MgSO4, 3.7% formaldehyde) 2h to over night at room temperature # store at –20C in 100% MeOH or EtOH (p..."
- 10:28, 22 August 2014 diff hist +835 N Labelling Pax6 with Dig-NHS Created page with "2 June 2005 Pax6 AB is at 5 mg/ml, 1 ml 1) Dialyze 500 µl into PBS O/N then one change in morning 2 hours. 2) Spin antibody and check protein concentration by BioRad STD ..."
- 08:02, 22 August 2014 diff hist +22 Thawing 293FT cells
- 07:56, 22 August 2014 diff hist -9 Thawing 293FT cells
- 07:52, 22 August 2014 diff hist +846 N Thawing 293FT cells Created page with "→ thaw the cells in prewarmed , complete medium without Geneticin materials: - a vial of frozen cells from the liquid nitrogen - 37ºC waterbath - 75 cm2 flask - HS for 293..."
- 09:43, 15 August 2014 diff hist +6 G418 medium preparation
- 09:41, 15 August 2014 diff hist +4 G418 medium preparation
- 09:39, 15 August 2014 diff hist +9 G418 medium preparation
- 09:37, 15 August 2014 diff hist +6 G418 medium preparation
- 09:35, 15 August 2014 diff hist +10 G418 medium preparation
- 09:33, 15 August 2014 diff hist +6 G418 medium preparation
- 09:30, 15 August 2014 diff hist +878 N G418 medium preparation Created page with "'''Materials:''' - 2x 15 ml FALCON tube - filter 0.22 µl - 5 ml syringe - DMEM - G418 '''Execution:''' 1 g of weight has a total concentration from 0,751 g How much of DMEM ..."
- 08:01, 11 August 2014 diff hist -11 Cardiomyocyte Preparation
- 08:00, 11 August 2014 diff hist +15 Cardiomyocyte Preparation
- 08:00, 11 August 2014 diff hist -4 Cardiomyocyte Preparation
- 07:59, 11 August 2014 diff hist 0 Cardiomyocyte Preparation
- 07:57, 11 August 2014 diff hist 0 Cardiomyocyte Preparation
- 07:55, 11 August 2014 diff hist -15 Cardiomyocyte Preparation
- 07:40, 11 August 2014 diff hist +1,449 N Cardiomyocyte Preparation Created page with "Bettencourt-Dias & Laube F combined 1) Collect hearts in 70% Leibovitz-15 (L15) medium (AL15) w/ pen-strep 2) Wash them once in 70% Leibovitz-15 (L15) medium (AL15) w/ pen-st..."
- 07:55, 6 August 2014 diff hist -1,895 Passaging A1 cell Blanked the page
- 07:53, 6 August 2014 diff hist +1,895 N A1 cell passaging myotube Created page with "<h4>For passaging 3 flasks of A1 cells:</h4> <li>1 flask into 3 flasks and <li>2 flasks into 2 x 10 cm plates (for making myotubes) <h3>Materials :</h3> <li>HS AMEM (see add..."
- 11:35, 4 August 2014 diff hist -1 Thawing (XB10) hybridoma cells
- 11:33, 4 August 2014 diff hist +1,150 Thawing (XB10) hybridoma cells
- 11:30, 4 August 2014 diff hist +15 Thawing (XB10) hybridoma cells
- 11:29, 4 August 2014 diff hist +427 Thawing (XB10) hybridoma cells
- 11:25, 4 August 2014 diff hist +14 N Thawing (XB10) hybridoma cells Created page with "==Materials:=="
- 11:23, 4 August 2014 diff hist +718 Hybridoma cells in SFX media
- 11:16, 4 August 2014 diff hist +201 N Hybridoma cells in SFX media Created page with "==Materials:== <li>5 ml, 10 ml, 20 ml pipettes <li>Serum free media( 400 mls SF media + 1% antibiotics 4 mls P/S and 4 mls Glutamine <li>2 blue cap..."
- 10:34, 2 July 2014 diff hist -1 Passaging A1 cell
- 10:33, 2 July 2014 diff hist -21 Passaging A1 cell
- 10:23, 2 July 2014 diff hist +21 Passaging A1 cell
- 09:12, 2 July 2014 diff hist -26 Passaging A1 cell
- 09:07, 2 July 2014 diff hist -5 Passaging A1 cell
- 09:04, 2 July 2014 diff hist +32 Passaging A1 cell
- 08:58, 2 July 2014 diff hist +9 Passaging A1 cell
- 08:57, 2 July 2014 diff hist +44 Passaging A1 cell
- 08:55, 2 July 2014 diff hist -86 Passaging A1 cell
- 08:52, 2 July 2014 diff hist +8 Passaging A1 cell
- 08:49, 2 July 2014 diff hist +9 Passaging A1 cell
- 08:41, 2 July 2014 diff hist +1 Passaging A1 cell
- 08:38, 2 July 2014 diff hist +1,910 N Passaging A1 cell Created page with "For passaging 3 flasks of A1 cells: 1 flask into 3 flasks and 2 flasks into 2 x10 cm plates (for making myotubes) Materials : HS AMEM (see additional protocol for recipe) ..."
- 09:34, 20 June 2014 diff hist +42 Letter to Froppier (French)
- 09:32, 20 June 2014 diff hist -42 Letter to Froppier (French)
- 09:30, 20 June 2014 diff hist +70 Letter to Froppier (French)
- 09:12, 20 June 2014 diff hist -9 Letter to Froppier (French)
- 09:08, 20 June 2014 diff hist -2 Letter to Froppier (French)
- 10:01, 18 June 2014 diff hist 0 Letter to Froppier (French)
- 10:00, 18 June 2014 diff hist 0 Letter to Froppier (French)
- 10:00, 18 June 2014 diff hist +1 Letter to Froppier (French)
- 09:57, 18 June 2014 diff hist +2 Letter to Froppier (French)
- 09:56, 18 June 2014 diff hist -8 Letter to Froppier (French)
- 09:56, 18 June 2014 diff hist +43 Letter to Froppier (French)
- 09:55, 18 June 2014 diff hist +20 Letter to Froppier (French)
- 09:53, 18 June 2014 diff hist +30 Letter to Froppier (French)
- 09:50, 18 June 2014 diff hist 0 Letter to Froppier (French)
- 09:49, 18 June 2014 diff hist +53 Letter to Froppier (French)
- 09:44, 18 June 2014 diff hist +3 Letter to Froppier (French)
- 09:42, 18 June 2014 diff hist -1 Letter to Froppier (French)
- 09:41, 18 June 2014 diff hist +89 Letter to Froppier (French)
- 09:30, 18 June 2014 diff hist +35 Letter to Froppier (French)
- 09:19, 18 June 2014 diff hist +733 N Letter to Froppier (French) Created page with "Max-Planck Institute Of Molecular Cell Biology and Genetics Pfotenhauerstrasse 108 01307 Dresden GERMANY G. FROPPIER Responsable Scientifique Elevage Scientifique des Dombes ..."
- 08:20, 11 June 2014 diff hist -2 BrdU and myosin staining →Materials:
- 08:19, 11 June 2014 diff hist -4 BrdU and myosin staining →Materials:
- 08:18, 11 June 2014 diff hist -6 BrdU and myosin staining →staining cells:
- 08:17, 11 June 2014 diff hist +20 BrdU and myosin staining →For staining cells:
- 08:15, 11 June 2014 diff hist +19 BrdU and myosin staining →Materials:
- 08:09, 11 June 2014 diff hist -15 BrdU and myosin staining
- 08:01, 11 June 2014 diff hist -14 BrdU and myosin staining
- 07:59, 11 June 2014 diff hist -7 BrdU and myosin staining
- 07:56, 11 June 2014 diff hist -2 BrdU and myosin staining
- 07:55, 11 June 2014 diff hist +3,204 N BrdU and myosin staining Created page with "== Fixing Cells: == 1 thaw 6% PFA in 37 C waterbath , place on ice 2 put 5 ml PBS + 5 ml 6% PFA in tray “PFA” , mix well 3 put 0.01% BSA/PBS into tray “BSA” ..."
- 08:27, 7 May 2014 diff hist +943 N Antibody staining Created page with "* Cut the tail and fix it in fresh 4% PFA in phosphate buffer from few hours to o/n always at 4C * Wash in PBS 1 hour at room temperature, put tail in 30% sucrose Put in OCT s..."
- 09:00, 2 May 2014 diff hist +252 BrdU labelling of A1 cells in 96-well plate
- 08:56, 2 May 2014 diff hist +102 N BrdU labelling of A1 cells in 96-well plate Created page with "== Materials: == * 5ml tube * SF MEM * BrDU drawer no. 3 * Multipipette * Pipette 100ul * Pipette 5ml"
- 08:46, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:45, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:36, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:34, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:32, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:29, 28 April 2014 diff hist +805 Passaging C2C12 cells
- 08:28, 28 April 2014 diff hist +17 Passaging C2C12 cells
- 06:58, 28 April 2014 diff hist +143 N Passaging C2C12 cells Created page with "== Materials: == * 1x 15ml FALCON tube * PBS w/o Ca2+ and Mg2+ * TE in PBS (4 ml TE + 36 ml PBS) * HS for C2C12 cells * pipettes * 75 cm2 flask"
- 09:32, 24 March 2014 diff hist +768 Purifying IgG1 from Hybridoma Supernatant
- 09:27, 24 March 2014 diff hist -45 Purifying IgG1 from Hybridoma Supernatant
- 09:25, 24 March 2014 diff hist +313 N Purifying IgG1 from Hybridoma Supernatant Created page with "<h3>Materials:</h3> <li>Supernatant (100-200 ml)</li> <li>Peristaltic pump</li> <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml)</li> <li>PBS (100 ml)</li> <li>10x ..."
- 12:48, 18 March 2014 diff hist -6 Overview →Protocols
- 12:45, 18 March 2014 diff hist +6 Overview →Antibody
- 12:42, 18 March 2014 diff hist +133 Overview →Protocols
- 12:19, 18 March 2014 diff hist +861 Labeled AB staining
- 12:12, 18 March 2014 diff hist +315 Labeled AB staining
- 12:06, 18 March 2014 diff hist +38 N Labeled AB staining Created page with "<h4>Pax7 + cy3 + NeuN – cy5Fab</h4>"
- 11:56, 18 March 2014 diff hist +50 Overview →Protocols
- 12:21, 10 March 2014 diff hist +10 RNA formaldehyde gels
- 12:17, 10 March 2014 diff hist +649 RNA formaldehyde gels
- 12:11, 10 March 2014 diff hist +205 RNA formaldehyde gels
- 12:06, 10 March 2014 diff hist +5 RNA formaldehyde gels
- 12:05, 10 March 2014 diff hist +248 N RNA formaldehyde gels Created page with "<h3>Prepare gel box: <li>Clean gel box and combs etc with detergent, then dionized water. <li>Then incubate the gel box and components in 10% H2O2 (hydrogen peroxide, stored i..."
- 12:01, 10 March 2014 diff hist +57 Overview →Molecular biology
- 08:52, 10 March 2014 diff hist -45 RNA Extraction from axolotl tissue
- 08:49, 10 March 2014 diff hist +755 RNA Extraction from axolotl tissue
- 08:43, 10 March 2014 diff hist +41 RNA Extraction from axolotl tissue
- 08:39, 10 March 2014 diff hist +56 RNA Extraction from axolotl tissue
- 08:34, 10 March 2014 diff hist +239 RNA Extraction from axolotl tissue
- 08:20, 10 March 2014 diff hist -1 RNA Extraction from axolotl tissue
- 08:20, 10 March 2014 diff hist +8 RNA Extraction from axolotl tissue
- 08:18, 10 March 2014 diff hist +12 N RNA Extraction from axolotl tissue Created page with " Materials:"
- 12:36, 6 March 2014 diff hist -5 Overview →Molecular biology
- 12:27, 6 March 2014 diff hist -3 Overview →Molecular biology
- 12:22, 6 March 2014 diff hist +89 Overview →Molecular biology
- 12:10, 6 March 2014 diff hist -102 Overview →RNA
- 09:30, 6 March 2014 diff hist +93 RNA extraction from axolotl tissue →Procedure to extract total RNA: current
- 09:21, 6 March 2014 diff hist +12 RNA extraction from axolotl tissue →Procedure to extract total RNA:
- 09:20, 6 March 2014 diff hist +1,242 N RNA extraction from axolotl tissue Created page with "== RNA Extraction from axolotl tissue == === Materials: === <ul> <li>Trizol (Invitrogen)</li> <li>Chloroform</li> <li>Isopropanol</li> <li>RNase free water</li> <..."
- 08:35, 6 March 2014 diff hist +95 Overview →Protocols
- 11:37, 17 January 2013 diff hist +7 Overview →Artemia Hatching Preparation: Protocol for 15 Ltr.
- 11:35, 17 January 2013 diff hist -1 Overview →Artemia Hatching Preparation: Protocol for 15 Ltr.
- 11:34, 17 January 2013 diff hist +370 Overview →Artemia Hatching Preparation: Protocol for 15 Ltr.
- 11:29, 17 January 2013 diff hist +62 Overview →Artemia (Brine shrimp eggs)
- 11:10, 17 January 2013 diff hist +78 Overview
- 11:00, 17 January 2013 diff hist -37 Overview
- 10:59, 17 January 2013 diff hist +37 Overview