Preparation of Freezing media for C2C12 cells

From Tanaka Wiki
Jump to: navigation, search

Material

  • 25 ml FCS (50%)
  • 20 ml DMEM + Pen/ Strep + Glut (40%)
  • 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro


Procedure

  1. put all stuffs together
  2. filtate the medium and aliquote the freezing media into 4 ml per tube
  3. store at –20ºC