Preparation of Low Serum for C2C12 cells

Material

• 390 mls DMEM-1 tube peni./ strepto.
• tube glutamine

(no insulin)

• 8 mls Horse serum-1 sterile bottle
• 500 ml sterile cylinder

	sterile filter 150 ml

Procedure

1. Put 390 mls DMEM into the cylinder
2. Than add Peni./Strepto. ,Glutamine (no insulin) Add 8 mls horse serum and than mix
3. filtrate the media through a sterile filter into the sterile bottle
4. Label the bottle with “C2C12, differentiation media” , name and date