Preparation of Low Serum for C2C12 cells

From Tanaka Wiki
Jump to: navigation, search

Material

  • 390 mls DMEM-1 tube peni./ strepto.
  • tube glutamine

(no insulin)

  • 8 mls Horse serum-1 sterile bottle
  • 500 ml sterile cylinder
	sterile filter 150 ml

Procedure

  1. Put 390 mls DMEM into the cylinder
  2. Than add Peni./Strepto. ,Glutamine (no insulin) Add 8 mls horse serum and than mix
  3. filtrate the media through a sterile filter into the sterile bottle
  4. Label the bottle with “C2C12, differentiation media” , name and date