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- 09:02, 27 March 2015 diff hist -1 C2C12 cell transfection with FuGene
- 08:57, 27 March 2015 diff hist 0 Myotube preparation of C2C12 cells
- 08:55, 27 March 2015 diff hist 0 Myotube preparation of C2C12 cells
- 08:52, 27 March 2015 diff hist +7 Myotube preparation of C2C12 cells
- 08:50, 27 March 2015 diff hist 0 Passaging C2C12 cells
- 08:48, 27 March 2015 diff hist +1 Preparation of High Serum for A1 cells
- 08:47, 27 March 2015 diff hist 0 BrdU and myosin staining →Materials
- 08:46, 27 March 2015 diff hist +7 BrdU and myosin staining →Materials
- 08:43, 27 March 2015 diff hist 0 Modified myotube prep
- 08:41, 27 March 2015 diff hist 0 Passaging C2C12 cells
- 08:39, 27 March 2015 diff hist -2 Passaging C2C12 cells
- 08:38, 27 March 2015 diff hist 0 Freezing of C2C12 cells
- 08:36, 27 March 2015 diff hist +2 Freezing of C2C12 cells
- 08:35, 27 March 2015 diff hist 0 Thawing frozen C2C12 cells
- 08:34, 27 March 2015 diff hist +2 Thawing frozen C2C12 cells
- 09:58, 25 March 2015 diff hist +16 Electroporation of blastema cells current
- 09:54, 25 March 2015 diff hist +2 Preparation of High Serum for A1 cells
- 09:53, 25 March 2015 diff hist +15 Preparation of High Serum for A1 cells
- 09:52, 25 March 2015 diff hist +11 Preparation of High Serum for A1 cells
- 09:50, 25 March 2015 diff hist 0 BrdU and myosin staining →Staining for BrDU and Myosin:
- 09:49, 25 March 2015 diff hist 0 BrdU and myosin staining →Staining for BrDU and Myosin:
- 09:46, 25 March 2015 diff hist -2 BrdU and myosin staining →Fixing Cells:
- 09:44, 25 March 2015 diff hist +21 BrdU and myosin staining
- 09:42, 25 March 2015 diff hist +3 BrdU and myosin staining
- 09:39, 25 March 2015 diff hist -2 BrdU labelling of A1 cells in 96-well plate
- 09:39, 25 March 2015 diff hist -3 Electroporation of A1 cells
- 09:38, 25 March 2015 diff hist +5 Electroporation of A1 cells
- 09:37, 25 March 2015 diff hist +2 Electroporation of A1 cells
- 09:36, 25 March 2015 diff hist -13 Electroporation of A1 cells
- 09:31, 25 March 2015 diff hist -35 A1 cell passaging myotube
- 09:27, 25 March 2015 diff hist -2 Passaging A1 cell
- 09:27, 25 March 2015 diff hist -4 Freezing of A1 cells
- 09:26, 25 March 2015 diff hist -2 Freezing of A1 cells
- 09:25, 25 March 2015 diff hist 0 Thawing frozen A1 cells
- 09:23, 25 March 2015 diff hist -2 Thawing frozen A1 cells
- 09:22, 25 March 2015 diff hist +4 Electroporation of nec current
- 09:21, 25 March 2015 diff hist +10 Electroporation of nec
- 09:19, 25 March 2015 diff hist -1 Cardiomyocyte Preparation current
- 09:17, 25 March 2015 diff hist -8 G418 medium preparation
- 09:16, 25 March 2015 diff hist -18 G418 medium preparation
- 09:13, 25 March 2015 diff hist -12 Thawing 293FT cells
- 09:11, 25 March 2015 diff hist -2 Myoblasts electroporation
- 09:07, 25 March 2015 diff hist -6 Electroporation of nec
- 09:05, 25 March 2015 diff hist 0 Preparation of Freezing media for C2C12 cells
- 09:05, 25 March 2015 diff hist 0 Preparation of High Serum for C2C12 cells
- 08:58, 25 March 2015 diff hist +10 Preparation of High Serum for C2C12 cells
- 08:55, 25 March 2015 diff hist +597 N Preparation of High Serum for C2C12 cells Created page with "Materials: - 1 tube pen/strep (green, 4 ml) - 1 tube glutamine (blue, 4 ml) → this is not necessary if the DMEM has glutamax - 1 bottle DMEM with 4500 mg/ l glucose - 40 ml..."
- 08:53, 25 March 2015 diff hist +16 Preparation of Freezing media for C2C12 cells
- 08:50, 25 March 2015 diff hist +247 N Preparation of Freezing media for C2C12 cells Created page with "Materials: 25 ml FCS (50%) 20 ml DMEM + Pen/ Strep + Glut (40%) 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro Procedure: - put all stuffs together - filtate the mediu..."
- 12:20, 23 March 2015 diff hist +6 A1 cell transfection with FuGene
- 12:18, 23 March 2015 diff hist +2 A1 cell transfection with FuGene
- 11:36, 23 March 2015 diff hist -11 A1 cell transfection with FuGene
- 11:32, 23 March 2015 diff hist -46 A1 cell transfection with FuGene
- 11:31, 23 March 2015 diff hist +49 A1 cell transfection with FuGene
- 11:30, 23 March 2015 diff hist -1 A1 cell transfection with FuGene
- 11:29, 23 March 2015 diff hist -2 A1 cell transfection with FuGene
- 11:28, 23 March 2015 diff hist -2 A1 cell transfection with FuGene
- 11:27, 23 March 2015 diff hist -1 A1 cell transfection with FuGene
- 11:27, 23 March 2015 diff hist +2 A1 cell transfection with FuGene
- 11:21, 23 March 2015 diff hist +1,463 N A1 cell transfection with FuGene Created page with "== Materials == * Fugene Transfection solution * Complete media for A1 cells * No serum media * ATE * APBS * 10 cm dishes * eppendorf tubes == Procedure == Incubation FuGen..."
- 08:47, 11 March 2015 diff hist +14 Cardiomyocyte Preparation
- 08:46, 11 March 2015 diff hist +1 Cardiomyocyte Preparation
- 08:44, 11 March 2015 diff hist -15 Cardiomyocyte Preparation
- 08:41, 11 March 2015 diff hist +4 Cardiomyocyte Preparation
- 08:40, 11 March 2015 diff hist +12 Cardiomyocyte Preparation
- 08:38, 11 March 2015 diff hist 0 Preparation of Low Serum for C2C12 cells
- 08:36, 11 March 2015 diff hist 0 Thawing 293FT cells
- 08:34, 11 March 2015 diff hist +2 Preparation of Low Serum for C2C12 cells
- 08:32, 11 March 2015 diff hist 0 Preparation of Low Serum for C2C12 cells
- 08:31, 11 March 2015 diff hist -8 Preparation of Low Serum for C2C12 cells
- 08:30, 11 March 2015 diff hist +5 Preparation of Low Serum for C2C12 cells
- 08:30, 11 March 2015 diff hist +475 N Preparation of Low Serum for C2C12 cells Created page with "== materials == <li> 390 mls DMEM-1 tube peni./ strepto. <li> tube glutamine (no insulin) <li> 8 mls Horse serum-1 sterile bottle <li> 500 ml sterile cylinder sterile fi..."
- 08:28, 16 February 2015 diff hist -51 Labelling myosin antibody
- 08:20, 16 February 2015 diff hist -2 Labelling myosin antibody
- 08:18, 16 February 2015 diff hist +20 Labelling myosin antibody
- 08:14, 16 February 2015 diff hist +4 Labelling myosin antibody
- 08:14, 16 February 2015 diff hist +36 Labelling myosin antibody
- 08:10, 16 February 2015 diff hist +978 N Labelling myosin antibody Created page with "0.2 M Borate buffer, pH 8 Biorad protein assay substance IgG standard Anti myosin antibody (4A1025) FlNHS (1.5 mg/ml in DMSO—make up fresh just before use) 100mM glycine, pH..."
- 10:25, 13 February 2015 diff hist -2 Preparation of High Serum for A1 cells
- 10:25, 13 February 2015 diff hist +3 Preparation of High Serum for A1 cells
- 10:24, 13 February 2015 diff hist +5 Preparation of High Serum for A1 cells
- 10:22, 13 February 2015 diff hist -1 Preparation of High Serum for A1 cells
- 10:20, 13 February 2015 diff hist 0 Preparation of High Serum for A1 cells
- 10:19, 13 February 2015 diff hist +7 Preparation of High Serum for A1 cells
- 10:16, 13 February 2015 diff hist +5 Preparation of High Serum for A1 cells
- 10:15, 13 February 2015 diff hist +16 Preparation of High Serum for A1 cells
- 10:09, 13 February 2015 diff hist +4 Preparation of High Serum for A1 cells
- 10:07, 13 February 2015 diff hist +465 N Preparation of High Serum for A1 cells Created page with "For 400 ml FCS 40 ml Eagle’s MEM 252 ml DdH2O 100 ml Pen/ Strep 4 ml Glutamin 4 ml Insulin (stock: 1mg/ ml) 4 ml - grow at 25ºC in 2% CO2 - if you want to gr..."
- 08:40, 13 February 2015 diff hist -14 Alcian Blue Staining
- 08:38, 13 February 2015 diff hist +599 N Alcian Blue Staining Created page with "# Fix sample O/N in 4% PFA freshly made # Wash in PBS, 3X5mins, 3X30mins 3. Wash 20mins in PBST 4. Equilibrate in EtOH, 25, 50%, 10 mins each 5. Place in Alcian blue staini..."
- 08:42, 9 February 2015 diff hist -2 Myoblasts electroporation
- 08:36, 9 February 2015 diff hist +6 Myoblasts electroporation
- 08:35, 9 February 2015 diff hist +4 Myoblasts electroporation
- 08:30, 9 February 2015 diff hist -9 Letter to Froppier (French) current
- 09:29, 2 February 2015 diff hist +265 Letter to Froppier (French)
- 13:00, 12 January 2015 diff hist +16 Freezing of A1 cells
- 12:54, 12 January 2015 diff hist +2 Electroporation of A1 cells
- 12:38, 12 January 2015 diff hist +2 Cloning of C2C12 cells
- 12:37, 12 January 2015 diff hist -7 Cloning of C2C12 cells
- 12:32, 12 January 2015 diff hist +7 Cloning of C2C12 cells