User contributions
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- 08:18, 29 June 2015 (diff | hist) . . (-6) . . Anti-BrDU Antibody labeling (→Procedure) (current)
- 08:16, 29 June 2015 (diff | hist) . . (+8) . . Anti-BrDU Antibody labeling (→Material)
- 08:13, 29 June 2015 (diff | hist) . . (+6) . . Anti-BrDU Antibody labeling (→Material)
- 08:13, 29 June 2015 (diff | hist) . . (-1) . . Anti-BrDU Antibody labeling
- 08:10, 29 June 2015 (diff | hist) . . (+1,562) . . N Anti-BrDU Antibody labeling (Created page with "Materials: Centricon-100 Rhodamine N-hydroxy-succiniymidyl ester (stored at -20) DMSO Antibody in 0.1M Borate buffer pH 8.8 NAP desalting columns or dialysis membrane. 0....")
- 08:09, 29 June 2015 (diff | hist) . . (+41) . . Overview (→Monoclonal Antibodies:)
- 08:06, 29 June 2015 (diff | hist) . . (-60) . . Overview (→Polyclonal Antibodies:)
- 07:57, 29 June 2015 (diff | hist) . . (-142) . . Address for making polyclonal antibodies (current)
- 07:57, 29 June 2015 (diff | hist) . . (+101) . . Address for making polyclonal antibodies
- 07:54, 29 June 2015 (diff | hist) . . (+28) . . Address for making polyclonal antibodies
- 07:52, 29 June 2015 (diff | hist) . . (+4) . . Address for making polyclonal antibodies
- 07:51, 29 June 2015 (diff | hist) . . (+2) . . Address for making polyclonal antibodies
- 07:51, 29 June 2015 (diff | hist) . . (+10) . . Address for making polyclonal antibodies
- 07:49, 29 June 2015 (diff | hist) . . (-2) . . Address for making polyclonal antibodies
- 07:48, 29 June 2015 (diff | hist) . . (0) . . Address for making polyclonal antibodies
- 07:48, 29 June 2015 (diff | hist) . . (+2) . . Address for making polyclonal antibodies
- 07:47, 29 June 2015 (diff | hist) . . (+97) . . N Address for making polyclonal antibodies (Created page with "Elevage Scientifique Des Dombes Romans 01400 chatillon sur chalaronne France Tel: 04.74.55.34.97")
- 07:45, 29 June 2015 (diff | hist) . . (-2) . . Overview (→Polyclonal Antibodies:)
- 14:01, 24 June 2015 (diff | hist) . . (-1) . . Baculovirus titration (current)
- 14:00, 24 June 2015 (diff | hist) . . (+30) . . Baculovirus titration
- 13:56, 24 June 2015 (diff | hist) . . (-414) . . Baculovirus titration
- 13:50, 24 June 2015 (diff | hist) . . (+3,084) . . N Baculovirus titration (Created page with "This protocol is not optimized yet. For example, adding SF+ cells before or after the virus seems to make a big difference… more troubleshooting is needed. Serial dilute th...")
- 08:31, 24 June 2015 (diff | hist) . . (-6) . . Overview (→Molecular biology)
- 08:30, 24 June 2015 (diff | hist) . . (-4) . . Overview (→Molecular biology)
- 08:29, 24 June 2015 (diff | hist) . . (+6) . . Overview (→Molecular biology)
- 08:28, 24 June 2015 (diff | hist) . . (-2) . . Overview (→Molecular biology)
- 08:25, 24 June 2015 (diff | hist) . . (-9) . . Overview (→Molecular biology)
- 08:05, 24 June 2015 (diff | hist) . . (-4) . . Neurosphere culture (current)
- 08:02, 24 June 2015 (diff | hist) . . (+35) . . Neurosphere culture
- 08:00, 24 June 2015 (diff | hist) . . (+12) . . Neurosphere culture
- 07:59, 24 June 2015 (diff | hist) . . (+6) . . Neurosphere culture
- 07:58, 24 June 2015 (diff | hist) . . (-3) . . Neurosphere culture
- 07:54, 24 June 2015 (diff | hist) . . (+6) . . Neurosphere culture
- 07:53, 24 June 2015 (diff | hist) . . (-12) . . Neurosphere culture
- 07:52, 24 June 2015 (diff | hist) . . (-24) . . Neurosphere culture
- 07:36, 24 June 2015 (diff | hist) . . (+1,685) . . N Neurosphere culture (Created page with "Neurosphere Culture of SC Dissect SC from 5cm animals (GFP) Place SC into L15 medium (Gibco Cat. No: 31415-029) Remove meninges and cut SC in smaller pieces Transfer SC into ...")
- 08:52, 19 June 2015 (diff | hist) . . (0) . . RNA Extraction from axolotl tissue (→Materials) (current)
- 08:51, 19 June 2015 (diff | hist) . . (-1) . . Labeled AB staining (→Materials) (current)
- 08:49, 19 June 2015 (diff | hist) . . (-1) . . Labelling myosin antibody (→Materials) (current)
- 08:48, 19 June 2015 (diff | hist) . . (-1) . . Purifying IgG1 from Hybridoma Supernatant (→Materials) (current)
- 08:47, 19 June 2015 (diff | hist) . . (-1) . . ISH on axolotl tissue sections (→Materials) (current)
- 08:46, 19 June 2015 (diff | hist) . . (+27) . . Double Immunostaining via antigen retrieval (→Procedure) (current)
- 14:16, 15 June 2015 (diff | hist) . . (-1) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5)) (current)
- 14:14, 15 June 2015 (diff | hist) . . (-2) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 14:13, 15 June 2015 (diff | hist) . . (+18) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 14:06, 15 June 2015 (diff | hist) . . (-11) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 14:01, 15 June 2015 (diff | hist) . . (+32) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 13:58, 15 June 2015 (diff | hist) . . (+30) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 13:56, 15 June 2015 (diff | hist) . . (+37) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 13:52, 15 June 2015 (diff | hist) . . (-2) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 13:52, 15 June 2015 (diff | hist) . . (-12) . . Hybridoma cells clones (→ELISA (step 1 & 4))
- 14:37, 8 June 2015 (diff | hist) . . (-2) . . Hybridoma cells clones
- 14:35, 8 June 2015 (diff | hist) . . (-1) . . Hybridoma cells in SFX media (current)
- 14:34, 8 June 2015 (diff | hist) . . (-1) . . Preparation of Freezing media for C2C12 cells (current)
- 14:34, 8 June 2015 (diff | hist) . . (-1) . . Preparation of High Serum for C2C12 cells (current)
- 14:33, 8 June 2015 (diff | hist) . . (-1) . . Preparation of Low Serum for C2C12 cells (current)
- 14:33, 8 June 2015 (diff | hist) . . (0) . . Electroporation of C2C12 cells (current)
- 14:32, 8 June 2015 (diff | hist) . . (-3) . . Cloning of C2C12 cells (current)
- 14:32, 8 June 2015 (diff | hist) . . (-1) . . C2C12 cell transfection with FuGene (current)
- 14:31, 8 June 2015 (diff | hist) . . (-1) . . Myotube preparation of C2C12 cells (current)
- 14:31, 8 June 2015 (diff | hist) . . (-1) . . Passaging C2C12 cells (current)
- 14:30, 8 June 2015 (diff | hist) . . (-1) . . Freezing of C2C12 cells (current)
- 14:30, 8 June 2015 (diff | hist) . . (-1) . . Thawing frozen C2C12 cells (current)
- 14:29, 8 June 2015 (diff | hist) . . (-1) . . Thawing (XB10) hybridoma cells (current)
- 14:29, 8 June 2015 (diff | hist) . . (-1) . . MESC culture (current)
- 14:28, 8 June 2015 (diff | hist) . . (-1) . . Preparation of Freezing media for A1 cells (current)
- 14:28, 8 June 2015 (diff | hist) . . (-1) . . Preparation of High Serum for A1 cells (→Materials) (current)
- 14:26, 8 June 2015 (diff | hist) . . (-1) . . BrdU and myosin staining (current)
- 14:26, 8 June 2015 (diff | hist) . . (-1) . . Preparation of Low Serum for A1 cells (current)
- 14:25, 8 June 2015 (diff | hist) . . (-1) . . BrdU labelling of A1 cells in 96-well plate (current)
- 14:25, 8 June 2015 (diff | hist) . . (-1) . . Electroporation of A1 cells (current)
- 14:24, 8 June 2015 (diff | hist) . . (-1) . . A1 cell transfection with FuGene (current)
- 14:24, 8 June 2015 (diff | hist) . . (-1) . . Modified myotube prep (current)
- 14:23, 8 June 2015 (diff | hist) . . (-1) . . A1 cell passaging myotube (current)
- 14:23, 8 June 2015 (diff | hist) . . (0) . . Passaging A1 cell (current)
- 14:21, 8 June 2015 (diff | hist) . . (-1) . . Passaging A1 cell
- 14:20, 8 June 2015 (diff | hist) . . (-1) . . Freezing of A1 cells (current)
- 14:20, 8 June 2015 (diff | hist) . . (-1) . . Thawing frozen A1 cells (current)
- 14:19, 8 June 2015 (diff | hist) . . (-1) . . G418 medium preparation (current)
- 14:18, 8 June 2015 (diff | hist) . . (-1) . . Thawing 293FT cells (current)
- 14:18, 8 June 2015 (diff | hist) . . (-1) . . Myoblasts electroporation (current)
- 14:17, 8 June 2015 (diff | hist) . . (-1) . . Adhesive cell culture (current)
- 14:17, 8 June 2015 (diff | hist) . . (+1) . . Adhesive cell culture
- 14:15, 8 June 2015 (diff | hist) . . (-2) . . Adhesive cell culture
- 14:12, 8 June 2015 (diff | hist) . . (-2) . . Adhesive cell culture
- 14:10, 8 June 2015 (diff | hist) . . (+28) . . Adhesive cell culture
- 14:06, 8 June 2015 (diff | hist) . . (+21) . . Adhesive cell culture
- 14:03, 8 June 2015 (diff | hist) . . (+17) . . Adhesive cell culture
- 13:55, 8 June 2015 (diff | hist) . . (+3,049) . . N Adhesive cell culture (Created page with "ECM coating and composition of culture media These steps for preparation were carried out under sterile terms in cell culture room at clean bench. Gelatine: Gelatine (Sigma,...")
- 08:49, 1 June 2015 (diff | hist) . . (0) . . TSA Staining on axolotl-tissue (current)
- 08:48, 1 June 2015 (diff | hist) . . (+6) . . TSA Staining on axolotl-tissue
- 08:40, 1 June 2015 (diff | hist) . . (-12) . . TSA Staining on axolotl-tissue
- 08:39, 1 June 2015 (diff | hist) . . (0) . . TSA Staining on axolotl-tissue
- 08:37, 1 June 2015 (diff | hist) . . (0) . . TSA Staining on axolotl-tissue
- 08:35, 1 June 2015 (diff | hist) . . (0) . . TSA Staining on axolotl-tissue
- 08:34, 1 June 2015 (diff | hist) . . (+12) . . TSA Staining on axolotl-tissue
- 08:28, 1 June 2015 (diff | hist) . . (-20) . . TSA Staining on axolotl-tissue
- 08:24, 1 June 2015 (diff | hist) . . (-2) . . TSA Staining on axolotl-tissue
- 08:23, 1 June 2015 (diff | hist) . . (+1) . . TSA Staining on axolotl-tissue
- 08:22, 1 June 2015 (diff | hist) . . (-6) . . TSA Staining on axolotl-tissue
- 08:19, 1 June 2015 (diff | hist) . . (-10) . . TSA Staining on axolotl-tissue
- 08:18, 1 June 2015 (diff | hist) . . (+10) . . TSA Staining on axolotl-tissue
- 08:17, 1 June 2015 (diff | hist) . . (-14) . . TSA Staining on axolotl-tissue
- 08:14, 1 June 2015 (diff | hist) . . (-6) . . TSA Staining on axolotl-tissue
- 08:12, 1 June 2015 (diff | hist) . . (0) . . TSA Staining on axolotl-tissue
- 08:06, 1 June 2015 (diff | hist) . . (+9) . . TSA Staining on axolotl-tissue
- 07:50, 1 June 2015 (diff | hist) . . (+1,096) . . N TSA Staining on axolotl-tissue (Created page with "Solutions: - TNT wash buffer: 0.1M TRIS-HCL, pH 7.5 0.15M NaCl 0.05% Tween20 - TNB buffer: a) for blocking: 0.1M TRIS-HCL, pH 7.5 0.15M NaCl 20% goat serum b) for di...")
- 14:11, 4 May 2015 (diff | hist) . . (-16) . . MESC culture
- 14:06, 4 May 2015 (diff | hist) . . (0) . . MESC culture
- 10:07, 4 May 2015 (diff | hist) . . (-11) . . MESC culture
- 10:06, 4 May 2015 (diff | hist) . . (-4) . . MESC culture
- 10:04, 4 May 2015 (diff | hist) . . (+19) . . MESC culture
- 10:02, 4 May 2015 (diff | hist) . . (+17) . . MESC culture
- 08:43, 4 May 2015 (diff | hist) . . (+4,160) . . N MESC culture (Created page with "46C-ES cells/cyst formation Thawing of 46C ES cells: Prepare the growth medium: 405 ml DMEM + high glucose * Glutamax (Invitrogen: 31966) 75 ml FCS 5 ml L-Gln 100x (Invitro...")
- 13:20, 17 April 2015 (diff | hist) . . (0) . . Electroporation of C2C12 cells
- 13:20, 17 April 2015 (diff | hist) . . (-1) . . Electroporation of C2C12 cells (→material)
- 13:17, 17 April 2015 (diff | hist) . . (0) . . Electroporation of C2C12 cells (→material)
- 13:15, 17 April 2015 (diff | hist) . . (+1,228) . . N Electroporation of C2C12 cells (Created page with "== material == Transfection * 1x Steinberger Medium * 1x DMEM without serum - remove the old media - wash the cells with 5 ml PBS - remove the PBS - add 2 ml TE - put the...")
- 12:58, 17 April 2015 (diff | hist) . . (0) . . Preparation of Freezing media for A1 cells (→Procedure)
- 12:54, 17 April 2015 (diff | hist) . . (+11) . . Preparation of Freezing media for A1 cells
- 12:52, 17 April 2015 (diff | hist) . . (+432) . . N Preparation of Freezing media for A1 cells (Created page with "Materials * DMSO (one ampules from Sigma) * High serum for A1 cells * 2x 50 ml FALCON tubes * 1x 15 ml FALCON tube * Pipettes ** 10x 5 ml FALCON tubes Procedure - clean the a...")
- 12:45, 17 April 2015 (diff | hist) . . (0) . . Preparation of Low Serum for A1 cells (→Procedure)
- 12:44, 17 April 2015 (diff | hist) . . (0) . . Preparation of Low Serum for A1 cells (→Materials)
- 12:40, 17 April 2015 (diff | hist) . . (+261) . . N Preparation of Low Serum for A1 cells (Created page with "== Materials == - 280 ml MEM - 100 ml sterile water - 1 tube Pen/ Strep (4ml) - 1 tube Glutamine (4ml) - 1 tube Insulin (4 ml) - 2 ml FCS - 500 ml filter with bottle == Proce...")
- 10:54, 27 March 2015 (diff | hist) . . (-22) . . RNA formaldehyde gels (current)
- 10:52, 27 March 2015 (diff | hist) . . (-3) . . RNA formaldehyde gels
- 10:51, 27 March 2015 (diff | hist) . . (+13) . . RNA formaldehyde gels
- 10:48, 27 March 2015 (diff | hist) . . (-17) . . RNA Extraction from axolotl tissue
- 10:45, 27 March 2015 (diff | hist) . . (-22) . . Digestion DNA (→Digestion) (current)
- 10:44, 27 March 2015 (diff | hist) . . (-28) . . Digestion DNA
- 10:42, 27 March 2015 (diff | hist) . . (+8) . . Digestion DNA
- 10:40, 27 March 2015 (diff | hist) . . (+16) . . Metamorphosis protocol (current)
- 10:39, 27 March 2015 (diff | hist) . . (+16) . . Antibody staining (current)
- 10:36, 27 March 2015 (diff | hist) . . (+11) . . Labeled AB staining
- 10:33, 27 March 2015 (diff | hist) . . (+4) . . Labelling Pax6 with Dig-NHS (current)
- 10:32, 27 March 2015 (diff | hist) . . (-5) . . Labelling myosin antibody
- 10:31, 27 March 2015 (diff | hist) . . (-18) . . Purifying IgG1 from Hybridoma Supernatant
- 10:30, 27 March 2015 (diff | hist) . . (-8) . . Purifying IgG1 from Hybridoma Supernatant
- 10:29, 27 March 2015 (diff | hist) . . (+16) . . Whole mount ISH on axolotl tissue (current)
- 10:27, 27 March 2015 (diff | hist) . . (-12) . . ISH on axolotl tissue sections (→Materials)
- 10:26, 27 March 2015 (diff | hist) . . (+31) . . ISH on axolotl tissue sections
- 10:23, 27 March 2015 (diff | hist) . . (+16) . . Double Immunostaining via antigen retrieval
- 10:22, 27 March 2015 (diff | hist) . . (+16) . . Alcian Blue Staining (current)
- 10:00, 27 March 2015 (diff | hist) . . (-23) . . Hybridoma cells in SFX media
- 09:59, 27 March 2015 (diff | hist) . . (-6) . . Hybridoma cells in SFX media
- 09:57, 27 March 2015 (diff | hist) . . (-14) . . Thawing (XB10) hybridoma cells
- 09:54, 27 March 2015 (diff | hist) . . (-14) . . Preparation of Low Serum for C2C12 cells
- 09:13, 27 March 2015 (diff | hist) . . (0) . . C2C12 cell transfection with FuGene (→Procedure)
- 09:06, 27 March 2015 (diff | hist) . . (+36) . . C2C12 cell transfection with FuGene
- 09:03, 27 March 2015 (diff | hist) . . (0) . . C2C12 cell transfection with FuGene (→Procedure)
- 09:02, 27 March 2015 (diff | hist) . . (-1) . . C2C12 cell transfection with FuGene
- 08:57, 27 March 2015 (diff | hist) . . (0) . . Myotube preparation of C2C12 cells
- 08:55, 27 March 2015 (diff | hist) . . (0) . . Myotube preparation of C2C12 cells
- 08:52, 27 March 2015 (diff | hist) . . (+7) . . Myotube preparation of C2C12 cells
- 08:50, 27 March 2015 (diff | hist) . . (0) . . Passaging C2C12 cells
- 08:48, 27 March 2015 (diff | hist) . . (+1) . . Preparation of High Serum for A1 cells
- 08:47, 27 March 2015 (diff | hist) . . (0) . . BrdU and myosin staining (→Materials)
- 08:46, 27 March 2015 (diff | hist) . . (+7) . . BrdU and myosin staining (→Materials)
- 08:43, 27 March 2015 (diff | hist) . . (0) . . Modified myotube prep
- 08:41, 27 March 2015 (diff | hist) . . (0) . . Passaging C2C12 cells
- 08:39, 27 March 2015 (diff | hist) . . (-2) . . Passaging C2C12 cells
- 08:38, 27 March 2015 (diff | hist) . . (0) . . Freezing of C2C12 cells
- 08:36, 27 March 2015 (diff | hist) . . (+2) . . Freezing of C2C12 cells
- 08:35, 27 March 2015 (diff | hist) . . (0) . . Thawing frozen C2C12 cells
- 08:34, 27 March 2015 (diff | hist) . . (+2) . . Thawing frozen C2C12 cells
- 09:58, 25 March 2015 (diff | hist) . . (+16) . . Electroporation of blastema cells (current)
- 09:54, 25 March 2015 (diff | hist) . . (+2) . . Preparation of High Serum for A1 cells
- 09:53, 25 March 2015 (diff | hist) . . (+15) . . Preparation of High Serum for A1 cells
- 09:52, 25 March 2015 (diff | hist) . . (+11) . . Preparation of High Serum for A1 cells
- 09:50, 25 March 2015 (diff | hist) . . (0) . . BrdU and myosin staining (→Staining for BrDU and Myosin:)
- 09:49, 25 March 2015 (diff | hist) . . (0) . . BrdU and myosin staining (→Staining for BrDU and Myosin:)
- 09:46, 25 March 2015 (diff | hist) . . (-2) . . BrdU and myosin staining (→Fixing Cells:)
- 09:44, 25 March 2015 (diff | hist) . . (+21) . . BrdU and myosin staining
- 09:42, 25 March 2015 (diff | hist) . . (+3) . . BrdU and myosin staining
- 09:39, 25 March 2015 (diff | hist) . . (-2) . . BrdU labelling of A1 cells in 96-well plate
- 09:39, 25 March 2015 (diff | hist) . . (-3) . . Electroporation of A1 cells
- 09:38, 25 March 2015 (diff | hist) . . (+5) . . Electroporation of A1 cells
- 09:37, 25 March 2015 (diff | hist) . . (+2) . . Electroporation of A1 cells
- 09:36, 25 March 2015 (diff | hist) . . (-13) . . Electroporation of A1 cells
- 09:31, 25 March 2015 (diff | hist) . . (-35) . . A1 cell passaging myotube
- 09:27, 25 March 2015 (diff | hist) . . (-2) . . Passaging A1 cell
- 09:27, 25 March 2015 (diff | hist) . . (-4) . . Freezing of A1 cells
- 09:26, 25 March 2015 (diff | hist) . . (-2) . . Freezing of A1 cells
- 09:25, 25 March 2015 (diff | hist) . . (0) . . Thawing frozen A1 cells
- 09:23, 25 March 2015 (diff | hist) . . (-2) . . Thawing frozen A1 cells
- 09:22, 25 March 2015 (diff | hist) . . (+4) . . Electroporation of nec (current)
- 09:21, 25 March 2015 (diff | hist) . . (+10) . . Electroporation of nec
- 09:19, 25 March 2015 (diff | hist) . . (-1) . . Cardiomyocyte Preparation (current)
- 09:17, 25 March 2015 (diff | hist) . . (-8) . . G418 medium preparation
- 09:16, 25 March 2015 (diff | hist) . . (-18) . . G418 medium preparation
- 09:13, 25 March 2015 (diff | hist) . . (-12) . . Thawing 293FT cells
- 09:11, 25 March 2015 (diff | hist) . . (-2) . . Myoblasts electroporation
- 09:07, 25 March 2015 (diff | hist) . . (-6) . . Electroporation of nec
- 09:05, 25 March 2015 (diff | hist) . . (0) . . Preparation of Freezing media for C2C12 cells
- 09:05, 25 March 2015 (diff | hist) . . (0) . . Preparation of High Serum for C2C12 cells
- 08:58, 25 March 2015 (diff | hist) . . (+10) . . Preparation of High Serum for C2C12 cells
- 08:55, 25 March 2015 (diff | hist) . . (+597) . . N Preparation of High Serum for C2C12 cells (Created page with "Materials: - 1 tube pen/strep (green, 4 ml) - 1 tube glutamine (blue, 4 ml) → this is not necessary if the DMEM has glutamax - 1 bottle DMEM with 4500 mg/ l glucose - 40 ml...")
- 08:53, 25 March 2015 (diff | hist) . . (+16) . . Preparation of Freezing media for C2C12 cells
- 08:50, 25 March 2015 (diff | hist) . . (+247) . . N Preparation of Freezing media for C2C12 cells (Created page with "Materials: 25 ml FCS (50%) 20 ml DMEM + Pen/ Strep + Glut (40%) 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro Procedure: - put all stuffs together - filtate the mediu...")
- 12:20, 23 March 2015 (diff | hist) . . (+6) . . A1 cell transfection with FuGene
- 12:18, 23 March 2015 (diff | hist) . . (+2) . . A1 cell transfection with FuGene
- 11:36, 23 March 2015 (diff | hist) . . (-11) . . A1 cell transfection with FuGene
- 11:32, 23 March 2015 (diff | hist) . . (-46) . . A1 cell transfection with FuGene
- 11:31, 23 March 2015 (diff | hist) . . (+49) . . A1 cell transfection with FuGene
- 11:30, 23 March 2015 (diff | hist) . . (-1) . . A1 cell transfection with FuGene
- 11:29, 23 March 2015 (diff | hist) . . (-2) . . A1 cell transfection with FuGene
- 11:28, 23 March 2015 (diff | hist) . . (-2) . . A1 cell transfection with FuGene
- 11:27, 23 March 2015 (diff | hist) . . (-1) . . A1 cell transfection with FuGene
- 11:27, 23 March 2015 (diff | hist) . . (+2) . . A1 cell transfection with FuGene
- 11:21, 23 March 2015 (diff | hist) . . (+1,463) . . N A1 cell transfection with FuGene (Created page with "== Materials == * Fugene Transfection solution * Complete media for A1 cells * No serum media * ATE * APBS * 10 cm dishes * eppendorf tubes == Procedure == Incubation FuGen...")
- 08:47, 11 March 2015 (diff | hist) . . (+14) . . Cardiomyocyte Preparation
- 08:46, 11 March 2015 (diff | hist) . . (+1) . . Cardiomyocyte Preparation
- 08:44, 11 March 2015 (diff | hist) . . (-15) . . Cardiomyocyte Preparation
- 08:41, 11 March 2015 (diff | hist) . . (+4) . . Cardiomyocyte Preparation
- 08:40, 11 March 2015 (diff | hist) . . (+12) . . Cardiomyocyte Preparation
- 08:38, 11 March 2015 (diff | hist) . . (0) . . Preparation of Low Serum for C2C12 cells
- 08:36, 11 March 2015 (diff | hist) . . (0) . . Thawing 293FT cells
- 08:34, 11 March 2015 (diff | hist) . . (+2) . . Preparation of Low Serum for C2C12 cells
- 08:32, 11 March 2015 (diff | hist) . . (0) . . Preparation of Low Serum for C2C12 cells
- 08:31, 11 March 2015 (diff | hist) . . (-8) . . Preparation of Low Serum for C2C12 cells
- 08:30, 11 March 2015 (diff | hist) . . (+5) . . Preparation of Low Serum for C2C12 cells
- 08:30, 11 March 2015 (diff | hist) . . (+475) . . N Preparation of Low Serum for C2C12 cells (Created page with "== materials == <li> 390 mls DMEM-1 tube peni./ strepto. <li> tube glutamine (no insulin) <li> 8 mls Horse serum-1 sterile bottle <li> 500 ml sterile cylinder sterile fi...")
- 08:28, 16 February 2015 (diff | hist) . . (-51) . . Labelling myosin antibody
- 08:20, 16 February 2015 (diff | hist) . . (-2) . . Labelling myosin antibody
- 08:18, 16 February 2015 (diff | hist) . . (+20) . . Labelling myosin antibody
- 08:14, 16 February 2015 (diff | hist) . . (+4) . . Labelling myosin antibody
- 08:14, 16 February 2015 (diff | hist) . . (+36) . . Labelling myosin antibody
- 08:10, 16 February 2015 (diff | hist) . . (+978) . . N Labelling myosin antibody (Created page with "0.2 M Borate buffer, pH 8 Biorad protein assay substance IgG standard Anti myosin antibody (4A1025) FlNHS (1.5 mg/ml in DMSO—make up fresh just before use) 100mM glycine, pH...")
- 10:25, 13 February 2015 (diff | hist) . . (-2) . . Preparation of High Serum for A1 cells
- 10:25, 13 February 2015 (diff | hist) . . (+3) . . Preparation of High Serum for A1 cells
- 10:24, 13 February 2015 (diff | hist) . . (+5) . . Preparation of High Serum for A1 cells
- 10:22, 13 February 2015 (diff | hist) . . (-1) . . Preparation of High Serum for A1 cells
- 10:20, 13 February 2015 (diff | hist) . . (0) . . Preparation of High Serum for A1 cells
- 10:19, 13 February 2015 (diff | hist) . . (+7) . . Preparation of High Serum for A1 cells
- 10:16, 13 February 2015 (diff | hist) . . (+5) . . Preparation of High Serum for A1 cells
- 10:15, 13 February 2015 (diff | hist) . . (+16) . . Preparation of High Serum for A1 cells
- 10:09, 13 February 2015 (diff | hist) . . (+4) . . Preparation of High Serum for A1 cells
- 10:07, 13 February 2015 (diff | hist) . . (+465) . . N Preparation of High Serum for A1 cells (Created page with "For 400 ml FCS 40 ml Eagle’s MEM 252 ml DdH2O 100 ml Pen/ Strep 4 ml Glutamin 4 ml Insulin (stock: 1mg/ ml) 4 ml - grow at 25ºC in 2% CO2 - if you want to gr...")
- 08:40, 13 February 2015 (diff | hist) . . (-14) . . Alcian Blue Staining
- 08:38, 13 February 2015 (diff | hist) . . (+599) . . N Alcian Blue Staining (Created page with "# Fix sample O/N in 4% PFA freshly made # Wash in PBS, 3X5mins, 3X30mins 3. Wash 20mins in PBST 4. Equilibrate in EtOH, 25, 50%, 10 mins each 5. Place in Alcian blue staini...")
- 08:42, 9 February 2015 (diff | hist) . . (-2) . . Myoblasts electroporation
- 08:36, 9 February 2015 (diff | hist) . . (+6) . . Myoblasts electroporation
- 08:35, 9 February 2015 (diff | hist) . . (+4) . . Myoblasts electroporation
- 08:30, 9 February 2015 (diff | hist) . . (-9) . . Letter to Froppier (French) (current)
- 09:29, 2 February 2015 (diff | hist) . . (+265) . . Letter to Froppier (French)
- 13:00, 12 January 2015 (diff | hist) . . (+16) . . Freezing of A1 cells
- 12:54, 12 January 2015 (diff | hist) . . (+2) . . Electroporation of A1 cells
- 12:38, 12 January 2015 (diff | hist) . . (+2) . . Cloning of C2C12 cells
- 12:37, 12 January 2015 (diff | hist) . . (-7) . . Cloning of C2C12 cells
- 12:32, 12 January 2015 (diff | hist) . . (+7) . . Cloning of C2C12 cells
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