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- 08:18, 29 June 2015 diff hist -6 Anti-BrDU Antibody labeling →Procedure current
- 08:16, 29 June 2015 diff hist +8 Anti-BrDU Antibody labeling →Material
- 08:13, 29 June 2015 diff hist +6 Anti-BrDU Antibody labeling →Material
- 08:13, 29 June 2015 diff hist -1 Anti-BrDU Antibody labeling
- 08:10, 29 June 2015 diff hist +1,562 N Anti-BrDU Antibody labeling Created page with "Materials: Centricon-100 Rhodamine N-hydroxy-succiniymidyl ester (stored at -20) DMSO Antibody in 0.1M Borate buffer pH 8.8 NAP desalting columns or dialysis membrane. 0...."
- 08:09, 29 June 2015 diff hist +41 Overview →Monoclonal Antibodies:
- 08:06, 29 June 2015 diff hist -60 Overview →Polyclonal Antibodies:
- 07:57, 29 June 2015 diff hist -142 Address for making polyclonal antibodies current
- 07:57, 29 June 2015 diff hist +101 Address for making polyclonal antibodies
- 07:54, 29 June 2015 diff hist +28 Address for making polyclonal antibodies
- 07:52, 29 June 2015 diff hist +4 Address for making polyclonal antibodies
- 07:51, 29 June 2015 diff hist +2 Address for making polyclonal antibodies
- 07:51, 29 June 2015 diff hist +10 Address for making polyclonal antibodies
- 07:49, 29 June 2015 diff hist -2 Address for making polyclonal antibodies
- 07:48, 29 June 2015 diff hist 0 Address for making polyclonal antibodies
- 07:48, 29 June 2015 diff hist +2 Address for making polyclonal antibodies
- 07:47, 29 June 2015 diff hist +97 N Address for making polyclonal antibodies Created page with "Elevage Scientifique Des Dombes Romans 01400 chatillon sur chalaronne France Tel: 04.74.55.34.97"
- 07:45, 29 June 2015 diff hist -2 Overview →Polyclonal Antibodies:
- 14:01, 24 June 2015 diff hist -1 Baculovirus titration current
- 14:00, 24 June 2015 diff hist +30 Baculovirus titration
- 13:56, 24 June 2015 diff hist -414 Baculovirus titration
- 13:50, 24 June 2015 diff hist +3,084 N Baculovirus titration Created page with "This protocol is not optimized yet. For example, adding SF+ cells before or after the virus seems to make a big difference… more troubleshooting is needed. Serial dilute th..."
- 08:31, 24 June 2015 diff hist -6 Overview →Molecular biology
- 08:30, 24 June 2015 diff hist -4 Overview →Molecular biology
- 08:29, 24 June 2015 diff hist +6 Overview →Molecular biology
- 08:28, 24 June 2015 diff hist -2 Overview →Molecular biology
- 08:25, 24 June 2015 diff hist -9 Overview →Molecular biology
- 08:05, 24 June 2015 diff hist -4 Neurosphere culture current
- 08:02, 24 June 2015 diff hist +35 Neurosphere culture
- 08:00, 24 June 2015 diff hist +12 Neurosphere culture
- 07:59, 24 June 2015 diff hist +6 Neurosphere culture
- 07:58, 24 June 2015 diff hist -3 Neurosphere culture
- 07:54, 24 June 2015 diff hist +6 Neurosphere culture
- 07:53, 24 June 2015 diff hist -12 Neurosphere culture
- 07:52, 24 June 2015 diff hist -24 Neurosphere culture
- 07:36, 24 June 2015 diff hist +1,685 N Neurosphere culture Created page with "Neurosphere Culture of SC Dissect SC from 5cm animals (GFP) Place SC into L15 medium (Gibco Cat. No: 31415-029) Remove meninges and cut SC in smaller pieces Transfer SC into ..."
- 08:52, 19 June 2015 diff hist 0 RNA Extraction from axolotl tissue →Materials current
- 08:51, 19 June 2015 diff hist -1 Labeled AB staining →Materials current
- 08:49, 19 June 2015 diff hist -1 Labelling myosin antibody →Materials current
- 08:48, 19 June 2015 diff hist -1 Purifying IgG1 from Hybridoma Supernatant →Materials current
- 08:47, 19 June 2015 diff hist -1 ISH on axolotl tissue sections →Materials current
- 08:46, 19 June 2015 diff hist +27 Double Immunostaining via antigen retrieval →Procedure current
- 14:16, 15 June 2015 diff hist -1 Hybridoma cells clones →Antibody Staining (step 2 & 5) current
- 14:14, 15 June 2015 diff hist -2 Hybridoma cells clones →Antibody Staining (step 2 & 5)
- 14:13, 15 June 2015 diff hist +18 Hybridoma cells clones →Antibody Staining (step 2 & 5)
- 14:06, 15 June 2015 diff hist -11 Hybridoma cells clones →Antibody Staining (step 2 & 5)
- 14:01, 15 June 2015 diff hist +32 Hybridoma cells clones →Antibody Staining (step 2 & 5)
- 13:58, 15 June 2015 diff hist +30 Hybridoma cells clones →Antibody Staining (step 2 & 5)
- 13:56, 15 June 2015 diff hist +37 Hybridoma cells clones →Antibody Staining (step 2 & 5)
- 13:52, 15 June 2015 diff hist -2 Hybridoma cells clones →Antibody Staining (step 2 & 5)
- 13:52, 15 June 2015 diff hist -12 Hybridoma cells clones →ELISA (step 1 & 4)
- 14:37, 8 June 2015 diff hist -2 Hybridoma cells clones
- 14:35, 8 June 2015 diff hist -1 Hybridoma cells in SFX media current
- 14:34, 8 June 2015 diff hist -1 Preparation of Freezing media for C2C12 cells current
- 14:34, 8 June 2015 diff hist -1 Preparation of High Serum for C2C12 cells current
- 14:33, 8 June 2015 diff hist -1 Preparation of Low Serum for C2C12 cells current
- 14:33, 8 June 2015 diff hist 0 Electroporation of C2C12 cells current
- 14:32, 8 June 2015 diff hist -3 Cloning of C2C12 cells current
- 14:32, 8 June 2015 diff hist -1 C2C12 cell transfection with FuGene current
- 14:31, 8 June 2015 diff hist -1 Myotube preparation of C2C12 cells current
- 14:31, 8 June 2015 diff hist -1 Passaging C2C12 cells current
- 14:30, 8 June 2015 diff hist -1 Freezing of C2C12 cells current
- 14:30, 8 June 2015 diff hist -1 Thawing frozen C2C12 cells current
- 14:29, 8 June 2015 diff hist -1 Thawing (XB10) hybridoma cells current
- 14:29, 8 June 2015 diff hist -1 MESC culture current
- 14:28, 8 June 2015 diff hist -1 Preparation of Freezing media for A1 cells current
- 14:28, 8 June 2015 diff hist -1 Preparation of High Serum for A1 cells →Materials current
- 14:26, 8 June 2015 diff hist -1 BrdU and myosin staining current
- 14:26, 8 June 2015 diff hist -1 Preparation of Low Serum for A1 cells current
- 14:25, 8 June 2015 diff hist -1 BrdU labelling of A1 cells in 96-well plate current
- 14:25, 8 June 2015 diff hist -1 Electroporation of A1 cells current
- 14:24, 8 June 2015 diff hist -1 A1 cell transfection with FuGene current
- 14:24, 8 June 2015 diff hist -1 Modified myotube prep current
- 14:23, 8 June 2015 diff hist -1 A1 cell passaging myotube current
- 14:23, 8 June 2015 diff hist 0 Passaging A1 cell current
- 14:21, 8 June 2015 diff hist -1 Passaging A1 cell
- 14:20, 8 June 2015 diff hist -1 Freezing of A1 cells current
- 14:20, 8 June 2015 diff hist -1 Thawing frozen A1 cells current
- 14:19, 8 June 2015 diff hist -1 G418 medium preparation current
- 14:18, 8 June 2015 diff hist -1 Thawing 293FT cells current
- 14:18, 8 June 2015 diff hist -1 Myoblasts electroporation current
- 14:17, 8 June 2015 diff hist -1 Adhesive cell culture current
- 14:17, 8 June 2015 diff hist +1 Adhesive cell culture
- 14:15, 8 June 2015 diff hist -2 Adhesive cell culture
- 14:12, 8 June 2015 diff hist -2 Adhesive cell culture
- 14:10, 8 June 2015 diff hist +28 Adhesive cell culture
- 14:06, 8 June 2015 diff hist +21 Adhesive cell culture
- 14:03, 8 June 2015 diff hist +17 Adhesive cell culture
- 13:55, 8 June 2015 diff hist +3,049 N Adhesive cell culture Created page with "ECM coating and composition of culture media These steps for preparation were carried out under sterile terms in cell culture room at clean bench. Gelatine: Gelatine (Sigma,..."
- 08:49, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue current
- 08:48, 1 June 2015 diff hist +6 TSA Staining on axolotl-tissue
- 08:40, 1 June 2015 diff hist -12 TSA Staining on axolotl-tissue
- 08:39, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue
- 08:37, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue
- 08:35, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue
- 08:34, 1 June 2015 diff hist +12 TSA Staining on axolotl-tissue
- 08:28, 1 June 2015 diff hist -20 TSA Staining on axolotl-tissue
- 08:24, 1 June 2015 diff hist -2 TSA Staining on axolotl-tissue
- 08:23, 1 June 2015 diff hist +1 TSA Staining on axolotl-tissue
- 08:22, 1 June 2015 diff hist -6 TSA Staining on axolotl-tissue
- 08:19, 1 June 2015 diff hist -10 TSA Staining on axolotl-tissue
- 08:18, 1 June 2015 diff hist +10 TSA Staining on axolotl-tissue
- 08:17, 1 June 2015 diff hist -14 TSA Staining on axolotl-tissue
- 08:14, 1 June 2015 diff hist -6 TSA Staining on axolotl-tissue
- 08:12, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue
- 08:06, 1 June 2015 diff hist +9 TSA Staining on axolotl-tissue
- 07:50, 1 June 2015 diff hist +1,096 N TSA Staining on axolotl-tissue Created page with "Solutions: - TNT wash buffer: 0.1M TRIS-HCL, pH 7.5 0.15M NaCl 0.05% Tween20 - TNB buffer: a) for blocking: 0.1M TRIS-HCL, pH 7.5 0.15M NaCl 20% goat serum b) for di..."
- 14:11, 4 May 2015 diff hist -16 MESC culture
- 14:06, 4 May 2015 diff hist 0 MESC culture
- 10:07, 4 May 2015 diff hist -11 MESC culture
- 10:06, 4 May 2015 diff hist -4 MESC culture
- 10:04, 4 May 2015 diff hist +19 MESC culture
- 10:02, 4 May 2015 diff hist +17 MESC culture
- 08:43, 4 May 2015 diff hist +4,160 N MESC culture Created page with "46C-ES cells/cyst formation Thawing of 46C ES cells: Prepare the growth medium: 405 ml DMEM + high glucose * Glutamax (Invitrogen: 31966) 75 ml FCS 5 ml L-Gln 100x (Invitro..."
- 13:20, 17 April 2015 diff hist 0 Electroporation of C2C12 cells
- 13:20, 17 April 2015 diff hist -1 Electroporation of C2C12 cells →material
- 13:17, 17 April 2015 diff hist 0 Electroporation of C2C12 cells →material
- 13:15, 17 April 2015 diff hist +1,228 N Electroporation of C2C12 cells Created page with "== material == Transfection * 1x Steinberger Medium * 1x DMEM without serum - remove the old media - wash the cells with 5 ml PBS - remove the PBS - add 2 ml TE - put the..."
- 12:58, 17 April 2015 diff hist 0 Preparation of Freezing media for A1 cells →Procedure
- 12:54, 17 April 2015 diff hist +11 Preparation of Freezing media for A1 cells
- 12:52, 17 April 2015 diff hist +432 N Preparation of Freezing media for A1 cells Created page with "Materials * DMSO (one ampules from Sigma) * High serum for A1 cells * 2x 50 ml FALCON tubes * 1x 15 ml FALCON tube * Pipettes ** 10x 5 ml FALCON tubes Procedure - clean the a..."
- 12:45, 17 April 2015 diff hist 0 Preparation of Low Serum for A1 cells →Procedure
- 12:44, 17 April 2015 diff hist 0 Preparation of Low Serum for A1 cells →Materials
- 12:40, 17 April 2015 diff hist +261 N Preparation of Low Serum for A1 cells Created page with "== Materials == - 280 ml MEM - 100 ml sterile water - 1 tube Pen/ Strep (4ml) - 1 tube Glutamine (4ml) - 1 tube Insulin (4 ml) - 2 ml FCS - 500 ml filter with bottle == Proce..."
- 10:54, 27 March 2015 diff hist -22 RNA formaldehyde gels current
- 10:52, 27 March 2015 diff hist -3 RNA formaldehyde gels
- 10:51, 27 March 2015 diff hist +13 RNA formaldehyde gels
- 10:48, 27 March 2015 diff hist -17 RNA Extraction from axolotl tissue
- 10:45, 27 March 2015 diff hist -22 Digestion DNA →Digestion current
- 10:44, 27 March 2015 diff hist -28 Digestion DNA
- 10:42, 27 March 2015 diff hist +8 Digestion DNA
- 10:40, 27 March 2015 diff hist +16 Metamorphosis protocol current
- 10:39, 27 March 2015 diff hist +16 Antibody staining current
- 10:36, 27 March 2015 diff hist +11 Labeled AB staining
- 10:33, 27 March 2015 diff hist +4 Labelling Pax6 with Dig-NHS current
- 10:32, 27 March 2015 diff hist -5 Labelling myosin antibody
- 10:31, 27 March 2015 diff hist -18 Purifying IgG1 from Hybridoma Supernatant
- 10:30, 27 March 2015 diff hist -8 Purifying IgG1 from Hybridoma Supernatant
- 10:29, 27 March 2015 diff hist +16 Whole mount ISH on axolotl tissue current
- 10:27, 27 March 2015 diff hist -12 ISH on axolotl tissue sections →Materials
- 10:26, 27 March 2015 diff hist +31 ISH on axolotl tissue sections
- 10:23, 27 March 2015 diff hist +16 Double Immunostaining via antigen retrieval
- 10:22, 27 March 2015 diff hist +16 Alcian Blue Staining current
- 10:00, 27 March 2015 diff hist -23 Hybridoma cells in SFX media
- 09:59, 27 March 2015 diff hist -6 Hybridoma cells in SFX media
- 09:57, 27 March 2015 diff hist -14 Thawing (XB10) hybridoma cells
- 09:54, 27 March 2015 diff hist -14 Preparation of Low Serum for C2C12 cells
- 09:13, 27 March 2015 diff hist 0 C2C12 cell transfection with FuGene →Procedure
- 09:06, 27 March 2015 diff hist +36 C2C12 cell transfection with FuGene
- 09:03, 27 March 2015 diff hist 0 C2C12 cell transfection with FuGene →Procedure
- 09:02, 27 March 2015 diff hist -1 C2C12 cell transfection with FuGene
- 08:57, 27 March 2015 diff hist 0 Myotube preparation of C2C12 cells
- 08:55, 27 March 2015 diff hist 0 Myotube preparation of C2C12 cells
- 08:52, 27 March 2015 diff hist +7 Myotube preparation of C2C12 cells
- 08:50, 27 March 2015 diff hist 0 Passaging C2C12 cells
- 08:48, 27 March 2015 diff hist +1 Preparation of High Serum for A1 cells
- 08:47, 27 March 2015 diff hist 0 BrdU and myosin staining →Materials
- 08:46, 27 March 2015 diff hist +7 BrdU and myosin staining →Materials
- 08:43, 27 March 2015 diff hist 0 Modified myotube prep
- 08:41, 27 March 2015 diff hist 0 Passaging C2C12 cells
- 08:39, 27 March 2015 diff hist -2 Passaging C2C12 cells
- 08:38, 27 March 2015 diff hist 0 Freezing of C2C12 cells
- 08:36, 27 March 2015 diff hist +2 Freezing of C2C12 cells
- 08:35, 27 March 2015 diff hist 0 Thawing frozen C2C12 cells
- 08:34, 27 March 2015 diff hist +2 Thawing frozen C2C12 cells
- 09:58, 25 March 2015 diff hist +16 Electroporation of blastema cells current
- 09:54, 25 March 2015 diff hist +2 Preparation of High Serum for A1 cells
- 09:53, 25 March 2015 diff hist +15 Preparation of High Serum for A1 cells
- 09:52, 25 March 2015 diff hist +11 Preparation of High Serum for A1 cells
- 09:50, 25 March 2015 diff hist 0 BrdU and myosin staining →Staining for BrDU and Myosin:
- 09:49, 25 March 2015 diff hist 0 BrdU and myosin staining →Staining for BrDU and Myosin:
- 09:46, 25 March 2015 diff hist -2 BrdU and myosin staining →Fixing Cells:
- 09:44, 25 March 2015 diff hist +21 BrdU and myosin staining
- 09:42, 25 March 2015 diff hist +3 BrdU and myosin staining
- 09:39, 25 March 2015 diff hist -2 BrdU labelling of A1 cells in 96-well plate
- 09:39, 25 March 2015 diff hist -3 Electroporation of A1 cells
- 09:38, 25 March 2015 diff hist +5 Electroporation of A1 cells
- 09:37, 25 March 2015 diff hist +2 Electroporation of A1 cells
- 09:36, 25 March 2015 diff hist -13 Electroporation of A1 cells
- 09:31, 25 March 2015 diff hist -35 A1 cell passaging myotube
- 09:27, 25 March 2015 diff hist -2 Passaging A1 cell
- 09:27, 25 March 2015 diff hist -4 Freezing of A1 cells
- 09:26, 25 March 2015 diff hist -2 Freezing of A1 cells
- 09:25, 25 March 2015 diff hist 0 Thawing frozen A1 cells
- 09:23, 25 March 2015 diff hist -2 Thawing frozen A1 cells
- 09:22, 25 March 2015 diff hist +4 Electroporation of nec current
- 09:21, 25 March 2015 diff hist +10 Electroporation of nec
- 09:19, 25 March 2015 diff hist -1 Cardiomyocyte Preparation current
- 09:17, 25 March 2015 diff hist -8 G418 medium preparation
- 09:16, 25 March 2015 diff hist -18 G418 medium preparation
- 09:13, 25 March 2015 diff hist -12 Thawing 293FT cells
- 09:11, 25 March 2015 diff hist -2 Myoblasts electroporation
- 09:07, 25 March 2015 diff hist -6 Electroporation of nec
- 09:05, 25 March 2015 diff hist 0 Preparation of Freezing media for C2C12 cells
- 09:05, 25 March 2015 diff hist 0 Preparation of High Serum for C2C12 cells
- 08:58, 25 March 2015 diff hist +10 Preparation of High Serum for C2C12 cells
- 08:55, 25 March 2015 diff hist +597 N Preparation of High Serum for C2C12 cells Created page with "Materials: - 1 tube pen/strep (green, 4 ml) - 1 tube glutamine (blue, 4 ml) → this is not necessary if the DMEM has glutamax - 1 bottle DMEM with 4500 mg/ l glucose - 40 ml..."
- 08:53, 25 March 2015 diff hist +16 Preparation of Freezing media for C2C12 cells
- 08:50, 25 March 2015 diff hist +247 N Preparation of Freezing media for C2C12 cells Created page with "Materials: 25 ml FCS (50%) 20 ml DMEM + Pen/ Strep + Glut (40%) 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro Procedure: - put all stuffs together - filtate the mediu..."
- 12:20, 23 March 2015 diff hist +6 A1 cell transfection with FuGene
- 12:18, 23 March 2015 diff hist +2 A1 cell transfection with FuGene
- 11:36, 23 March 2015 diff hist -11 A1 cell transfection with FuGene
- 11:32, 23 March 2015 diff hist -46 A1 cell transfection with FuGene
- 11:31, 23 March 2015 diff hist +49 A1 cell transfection with FuGene
- 11:30, 23 March 2015 diff hist -1 A1 cell transfection with FuGene
- 11:29, 23 March 2015 diff hist -2 A1 cell transfection with FuGene
- 11:28, 23 March 2015 diff hist -2 A1 cell transfection with FuGene
- 11:27, 23 March 2015 diff hist -1 A1 cell transfection with FuGene
- 11:27, 23 March 2015 diff hist +2 A1 cell transfection with FuGene
- 11:21, 23 March 2015 diff hist +1,463 N A1 cell transfection with FuGene Created page with "== Materials == * Fugene Transfection solution * Complete media for A1 cells * No serum media * ATE * APBS * 10 cm dishes * eppendorf tubes == Procedure == Incubation FuGen..."
- 08:47, 11 March 2015 diff hist +14 Cardiomyocyte Preparation
- 08:46, 11 March 2015 diff hist +1 Cardiomyocyte Preparation
- 08:44, 11 March 2015 diff hist -15 Cardiomyocyte Preparation
- 08:41, 11 March 2015 diff hist +4 Cardiomyocyte Preparation
- 08:40, 11 March 2015 diff hist +12 Cardiomyocyte Preparation
- 08:38, 11 March 2015 diff hist 0 Preparation of Low Serum for C2C12 cells
- 08:36, 11 March 2015 diff hist 0 Thawing 293FT cells
- 08:34, 11 March 2015 diff hist +2 Preparation of Low Serum for C2C12 cells
- 08:32, 11 March 2015 diff hist 0 Preparation of Low Serum for C2C12 cells
- 08:31, 11 March 2015 diff hist -8 Preparation of Low Serum for C2C12 cells
- 08:30, 11 March 2015 diff hist +5 Preparation of Low Serum for C2C12 cells
- 08:30, 11 March 2015 diff hist +475 N Preparation of Low Serum for C2C12 cells Created page with "== materials == <li> 390 mls DMEM-1 tube peni./ strepto. <li> tube glutamine (no insulin) <li> 8 mls Horse serum-1 sterile bottle <li> 500 ml sterile cylinder sterile fi..."
- 08:28, 16 February 2015 diff hist -51 Labelling myosin antibody
- 08:20, 16 February 2015 diff hist -2 Labelling myosin antibody
- 08:18, 16 February 2015 diff hist +20 Labelling myosin antibody
- 08:14, 16 February 2015 diff hist +4 Labelling myosin antibody
- 08:14, 16 February 2015 diff hist +36 Labelling myosin antibody
- 08:10, 16 February 2015 diff hist +978 N Labelling myosin antibody Created page with "0.2 M Borate buffer, pH 8 Biorad protein assay substance IgG standard Anti myosin antibody (4A1025) FlNHS (1.5 mg/ml in DMSO—make up fresh just before use) 100mM glycine, pH..."
- 10:25, 13 February 2015 diff hist -2 Preparation of High Serum for A1 cells
- 10:25, 13 February 2015 diff hist +3 Preparation of High Serum for A1 cells
- 10:24, 13 February 2015 diff hist +5 Preparation of High Serum for A1 cells
- 10:22, 13 February 2015 diff hist -1 Preparation of High Serum for A1 cells
- 10:20, 13 February 2015 diff hist 0 Preparation of High Serum for A1 cells
- 10:19, 13 February 2015 diff hist +7 Preparation of High Serum for A1 cells
- 10:16, 13 February 2015 diff hist +5 Preparation of High Serum for A1 cells
- 10:15, 13 February 2015 diff hist +16 Preparation of High Serum for A1 cells
- 10:09, 13 February 2015 diff hist +4 Preparation of High Serum for A1 cells
- 10:07, 13 February 2015 diff hist +465 N Preparation of High Serum for A1 cells Created page with "For 400 ml FCS 40 ml Eagle’s MEM 252 ml DdH2O 100 ml Pen/ Strep 4 ml Glutamin 4 ml Insulin (stock: 1mg/ ml) 4 ml - grow at 25ºC in 2% CO2 - if you want to gr..."
- 08:40, 13 February 2015 diff hist -14 Alcian Blue Staining
- 08:38, 13 February 2015 diff hist +599 N Alcian Blue Staining Created page with "# Fix sample O/N in 4% PFA freshly made # Wash in PBS, 3X5mins, 3X30mins 3. Wash 20mins in PBST 4. Equilibrate in EtOH, 25, 50%, 10 mins each 5. Place in Alcian blue staini..."
- 08:42, 9 February 2015 diff hist -2 Myoblasts electroporation
- 08:36, 9 February 2015 diff hist +6 Myoblasts electroporation
- 08:35, 9 February 2015 diff hist +4 Myoblasts electroporation
- 08:30, 9 February 2015 diff hist -9 Letter to Froppier (French) current
- 09:29, 2 February 2015 diff hist +265 Letter to Froppier (French)
- 13:00, 12 January 2015 diff hist +16 Freezing of A1 cells
- 12:54, 12 January 2015 diff hist +2 Electroporation of A1 cells
- 12:38, 12 January 2015 diff hist +2 Cloning of C2C12 cells
- 12:37, 12 January 2015 diff hist -7 Cloning of C2C12 cells
- 12:32, 12 January 2015 diff hist +7 Cloning of C2C12 cells
- 12:29, 12 January 2015 diff hist +4 Cloning of C2C12 cells
- 12:28, 12 January 2015 diff hist +2,085 Cloning of C2C12 cells
- 12:24, 12 January 2015 diff hist +1 N Cloning of C2C12 cells Created page with "#"
- 08:40, 12 January 2015 diff hist -1 Freezing of A1 cells
- 08:35, 12 January 2015 diff hist -1 Myoblasts electroporation
- 08:30, 12 January 2015 diff hist +654 N Freezing of A1 cells Created page with "''in one 175 cm2 flask'' Materials: - APBS (100 ml PBS + 25 ml diwater) - TE in APBS (36 ml APBS + 4 ml TE) - 1x 15 ml FALCON tubes - Pipettes - 1 cryo tube - freezing media ..."
- 12:58, 3 November 2014 diff hist -4 C2C12 cell transfection with FuGene
- 12:56, 3 November 2014 diff hist +28 C2C12 cell transfection with FuGene
- 12:52, 3 November 2014 diff hist +2 C2C12 cell transfection with FuGene
- 12:51, 3 November 2014 diff hist 0 C2C12 cell transfection with FuGene
- 12:47, 3 November 2014 diff hist 0 C2C12 cell transfection with FuGene
- 12:43, 3 November 2014 diff hist +1 C2C12 cell transfection with FuGene
- 12:41, 3 November 2014 diff hist -4 C2C12 cell transfection with FuGene
- 12:38, 3 November 2014 diff hist +2 C2C12 cell transfection with FuGene
- 12:36, 3 November 2014 diff hist -42 C2C12 cell transfection with FuGene
- 12:34, 3 November 2014 diff hist +9 C2C12 cell transfection with FuGene
- 12:33, 3 November 2014 diff hist -1 C2C12 cell transfection with FuGene
- 12:31, 3 November 2014 diff hist 0 C2C12 cell transfection with FuGene
- 12:29, 3 November 2014 diff hist +12 C2C12 cell transfection with FuGene
- 12:28, 3 November 2014 diff hist -59 C2C12 cell transfection with FuGene
- 12:19, 3 November 2014 diff hist +3 C2C12 cell transfection with FuGene
- 12:19, 3 November 2014 diff hist +127 C2C12 cell transfection with FuGene
- 12:17, 3 November 2014 diff hist +1 C2C12 cell transfection with FuGene
- 12:16, 3 November 2014 diff hist -1 C2C12 cell transfection with FuGene
- 12:15, 3 November 2014 diff hist -2 C2C12 cell transfection with FuGene
- 12:15, 3 November 2014 diff hist +3 C2C12 cell transfection with FuGene
- 12:14, 3 November 2014 diff hist -1 C2C12 cell transfection with FuGene
- 10:55, 3 November 2014 diff hist +1 C2C12 cell transfection with FuGene
- 10:55, 3 November 2014 diff hist -21 C2C12 cell transfection with FuGene
- 10:52, 3 November 2014 diff hist +7 C2C12 cell transfection with FuGene
- 10:48, 3 November 2014 diff hist +2 C2C12 cell transfection with FuGene
- 10:46, 3 November 2014 diff hist +14 C2C12 cell transfection with FuGene
- 10:35, 3 November 2014 diff hist +131 C2C12 cell transfection with FuGene
- 10:30, 3 November 2014 diff hist +12 C2C12 cell transfection with FuGene
- 10:28, 3 November 2014 diff hist +4 C2C12 cell transfection with FuGene
- 10:25, 3 November 2014 diff hist +3,695 N C2C12 cell transfection with FuGene Created page with "2 x 6cm dishes with C2C12 cells Materials: Day1: 2x 6cm dishes 75 cm2 flask C2C12 cells in a 75 cm2 flask 15 ml FALCON tubes High serum media for C2C12 TE in PBS PBS ..."
- 07:41, 24 October 2014 diff hist 0 Freezing of C2C12 cells
- 07:35, 24 October 2014 diff hist -1 Freezing of C2C12 cells
- 07:35, 24 October 2014 diff hist +8 Freezing of C2C12 cells
- 07:33, 24 October 2014 diff hist -1 Freezing of C2C12 cells
- 07:25, 24 October 2014 diff hist +707 N Freezing of C2C12 cells Created page with "Materials: - 15 ml FALCON tube - PBS w/o Ca2+ and Mg2+ - TE in PBS - Freezing media (10% DMSO + 40% serum + 50% DMEM) - Pipettes and tips - Glas pipetts - cryo tube - ice - f..."
- 10:13, 22 October 2014 diff hist +3 Hybridoma cells clones
- 10:12, 22 October 2014 diff hist -3 Hybridoma cells clones
- 10:10, 22 October 2014 diff hist +2 Hybridoma cells clones →Procedure:
- 09:59, 22 October 2014 diff hist +2 Hybridoma cells clones
- 09:56, 22 October 2014 diff hist -40 Hybridoma cells clones →Procedure:
- 09:55, 22 October 2014 diff hist +38 Hybridoma cells clones →Procedure:
- 09:54, 22 October 2014 diff hist -20 Hybridoma cells clones
- 09:49, 22 October 2014 diff hist -6 Hybridoma cells clones
- 09:45, 22 October 2014 diff hist -7 Hybridoma cells clones
- 09:41, 22 October 2014 diff hist +8 Hybridoma cells clones
- 09:36, 22 October 2014 diff hist +12 Hybridoma cells clones
- 09:35, 22 October 2014 diff hist +5 Hybridoma cells clones
- 09:34, 22 October 2014 diff hist -2 Hybridoma cells clones
- 09:31, 22 October 2014 diff hist -7 Hybridoma cells clones
- 09:30, 22 October 2014 diff hist -24 Hybridoma cells clones
- 09:23, 22 October 2014 diff hist +11,002 N Hybridoma cells clones Created page with "== General execution: == 1) ELISA of the supernatants 2) Antibody staining on cryostat section (7 dpa tail sections) of the positive clones from the ELISA 3) Cloning of a p..."
- 07:44, 20 October 2014 diff hist +115 ISH on axolotl tissue sections
- 07:40, 20 October 2014 diff hist -6 ISH on axolotl tissue sections
- 07:39, 20 October 2014 diff hist -2 ISH on axolotl tissue sections
- 07:38, 20 October 2014 diff hist -4 ISH on axolotl tissue sections
- 07:34, 20 October 2014 diff hist +1 ISH on axolotl tissue sections
- 07:29, 20 October 2014 diff hist +1 ISH on axolotl tissue sections
- 07:26, 20 October 2014 diff hist +12 ISH on axolotl tissue sections
- 11:57, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:57, 13 October 2014 diff hist +15 ISH on axolotl tissue sections
- 11:55, 13 October 2014 diff hist +8 ISH on axolotl tissue sections
- 11:48, 13 October 2014 diff hist +3 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist -7 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist +13 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:46, 13 October 2014 diff hist +18 ISH on axolotl tissue sections
- 11:45, 13 October 2014 diff hist 0 ISH on axolotl tissue sections
- 11:44, 13 October 2014 diff hist -8 ISH on axolotl tissue sections
- 11:42, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:41, 13 October 2014 diff hist -1 ISH on axolotl tissue sections
- 11:40, 13 October 2014 diff hist -5 ISH on axolotl tissue sections
- 11:39, 13 October 2014 diff hist -5 ISH on axolotl tissue sections
- 11:35, 13 October 2014 diff hist +5,086 N ISH on axolotl tissue sections Created page with "== Probe preparation == 1. Mix the following at RT: 10x transcription buffer 2 µl dig Nucleotide mix 2 µl Linearized plasmid x µl (1µg) RNase inhibito..."
- 08:02, 10 October 2014 diff hist +1 Modified myotube prep
- 08:01, 10 October 2014 diff hist +7 Modified myotube prep
- 08:00, 10 October 2014 diff hist +4 Modified myotube prep
- 07:59, 10 October 2014 diff hist +1 Cardiomyocyte Preparation
- 07:58, 10 October 2014 diff hist -3 Cardiomyocyte Preparation
- 07:56, 10 October 2014 diff hist -1 Cardiomyocyte Preparation
- 07:54, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:54, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:53, 10 October 2014 diff hist -2 Cardiomyocyte Preparation
- 07:52, 10 October 2014 diff hist +4 Cardiomyocyte Preparation
- 07:51, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:50, 10 October 2014 diff hist +6 Cardiomyocyte Preparation
- 07:46, 10 October 2014 diff hist -10 Modified myotube prep
- 07:43, 10 October 2014 diff hist -56 Modified myotube prep
- 07:37, 10 October 2014 diff hist 0 Modified myotube prep →Execution
- 07:36, 10 October 2014 diff hist +5 Modified myotube prep
- 07:34, 10 October 2014 diff hist -38 Modified myotube prep
- 07:29, 10 October 2014 diff hist -1 Modified myotube prep
- 07:10, 10 October 2014 diff hist +7 Modified myotube prep
- 07:05, 10 October 2014 diff hist +3,246 N Modified myotube prep Created page with "Induce A1 cells on 10 cm plates to form myotubes by * wash 1x with 5 mls APBS * changing the media to 0.5% serum-AMEM * wait 4 days If desired, myotubes can be purified awa..."
- 08:09, 26 September 2014 diff hist +4 Passaging A1 cell
- 08:07, 26 September 2014 diff hist +6 Passaging A1 cell
- 08:06, 26 September 2014 diff hist +787 Passaging A1 cell
- 07:44, 24 September 2014 diff hist +4 Thawing frozen A1 cells
- 07:43, 24 September 2014 diff hist 0 Thawing frozen A1 cells
- 07:37, 24 September 2014 diff hist +828 N Thawing frozen A1 cells Created page with "== Materials: == - Gelatin (warmed to 37ºC) - HS for A1 cells - 1x 15 ml FALCON tubes - Pipettes - 1x 175 cm2 flasks - frozen cells into liquid nitrogen == Procedure: == - p..."
- 08:45, 5 September 2014 diff hist +2 Electroporation of A1 cells
- 08:45, 5 September 2014 diff hist +15 Electroporation of A1 cells
- 08:43, 5 September 2014 diff hist +11 Electroporation of A1 cells
- 08:41, 5 September 2014 diff hist +14 Electroporation of A1 cells
- 08:36, 5 September 2014 diff hist 0 Electroporation of A1 cells
- 08:35, 5 September 2014 diff hist +7 Electroporation of A1 cells
- 08:16, 5 September 2014 diff hist -3 Electroporation of A1 cells
- 08:14, 5 September 2014 diff hist -4 Electroporation of A1 cells
- 08:12, 5 September 2014 diff hist +100 Electroporation of A1 cells
- 07:26, 5 September 2014 diff hist +3 Electroporation of A1 cells
- 07:24, 5 September 2014 diff hist 0 Electroporation of A1 cells
- 07:23, 5 September 2014 diff hist -5 Electroporation of A1 cells
- 07:22, 5 September 2014 diff hist -13 Electroporation of A1 cells
- 07:20, 5 September 2014 diff hist +12 Electroporation of A1 cells
- 07:19, 5 September 2014 diff hist +4 Electroporation of A1 cells
- 07:18, 5 September 2014 diff hist +899 N Electroporation of A1 cells Created page with "==Materials:== Ice 4 mM cuvettes, N+1 cuvettes Steinberg's, at 4 C. Square pulse electroporator (we use the BTX 830 Squarporator) 1) Put cuvettes on ice, and cool down..."
- 09:33, 3 September 2014 diff hist 0 Thawing frozen C2C12 cells
- 09:31, 3 September 2014 diff hist +711 N Thawing frozen C2C12 cells Created page with "==Materials:== - frozen C2C12 cells - HS for C2C12 cells - pipettes and tips - FALCON tube 15 ml - Cell culture flask ==Procedure:== - heat the HS up to 37ºC - pipette 14 ml..."
- 09:09, 3 September 2014 diff hist 0 Myotube preparation of C2C12 cells
- 09:05, 3 September 2014 diff hist 0 Myotube preparation of C2C12 cells
- 09:03, 3 September 2014 diff hist +8 Myotube preparation of C2C12 cells
- 09:02, 3 September 2014 diff hist +2,773 N Myotube preparation of C2C12 cells Created page with "Materials for 1 x 96 well plate: - DMEM (3.5 ml) –purely - Fibronectin (final diluted concentration should be 20 ng/µl)) - 1x 96 well plate - 2% Horse Serum – Low Serum (..."
- 07:17, 29 August 2014 diff hist -6 Whole mount ISH on axolotl tissue
- 07:15, 29 August 2014 diff hist -24 Whole mount ISH on axolotl tissue
- 07:14, 29 August 2014 diff hist +1 Whole mount ISH on axolotl tissue
- 07:14, 29 August 2014 diff hist +3 Whole mount ISH on axolotl tissue
- 07:13, 29 August 2014 diff hist +1 Whole mount ISH on axolotl tissue
- 07:12, 29 August 2014 diff hist +19 Whole mount ISH on axolotl tissue
- 07:08, 29 August 2014 diff hist +3 Whole mount ISH on axolotl tissue
- 07:06, 29 August 2014 diff hist +6 Whole mount ISH on axolotl tissue
- 07:04, 29 August 2014 diff hist -40 Whole mount ISH on axolotl tissue
- 07:00, 29 August 2014 diff hist +3,046 N Whole mount ISH on axolotl tissue Created page with "# fix embryo or tail blastema in MEMFA (0.1M MOPS pH7.4, 2mM EGTA, 1mM MgSO4, 3.7% formaldehyde) 2h to over night at room temperature # store at –20C in 100% MeOH or EtOH (p..."
- 10:28, 22 August 2014 diff hist +835 N Labelling Pax6 with Dig-NHS Created page with "2 June 2005 Pax6 AB is at 5 mg/ml, 1 ml 1) Dialyze 500 µl into PBS O/N then one change in morning 2 hours. 2) Spin antibody and check protein concentration by BioRad STD ..."
- 08:02, 22 August 2014 diff hist +22 Thawing 293FT cells
- 07:56, 22 August 2014 diff hist -9 Thawing 293FT cells
- 07:52, 22 August 2014 diff hist +846 N Thawing 293FT cells Created page with "→ thaw the cells in prewarmed , complete medium without Geneticin materials: - a vial of frozen cells from the liquid nitrogen - 37ºC waterbath - 75 cm2 flask - HS for 293..."
- 09:43, 15 August 2014 diff hist +6 G418 medium preparation
- 09:41, 15 August 2014 diff hist +4 G418 medium preparation
- 09:39, 15 August 2014 diff hist +9 G418 medium preparation
- 09:37, 15 August 2014 diff hist +6 G418 medium preparation
- 09:35, 15 August 2014 diff hist +10 G418 medium preparation
- 09:33, 15 August 2014 diff hist +6 G418 medium preparation
- 09:30, 15 August 2014 diff hist +878 N G418 medium preparation Created page with "'''Materials:''' - 2x 15 ml FALCON tube - filter 0.22 µl - 5 ml syringe - DMEM - G418 '''Execution:''' 1 g of weight has a total concentration from 0,751 g How much of DMEM ..."
- 08:01, 11 August 2014 diff hist -11 Cardiomyocyte Preparation
- 08:00, 11 August 2014 diff hist +15 Cardiomyocyte Preparation
- 08:00, 11 August 2014 diff hist -4 Cardiomyocyte Preparation
- 07:59, 11 August 2014 diff hist 0 Cardiomyocyte Preparation
- 07:57, 11 August 2014 diff hist 0 Cardiomyocyte Preparation
- 07:55, 11 August 2014 diff hist -15 Cardiomyocyte Preparation
- 07:40, 11 August 2014 diff hist +1,449 N Cardiomyocyte Preparation Created page with "Bettencourt-Dias & Laube F combined 1) Collect hearts in 70% Leibovitz-15 (L15) medium (AL15) w/ pen-strep 2) Wash them once in 70% Leibovitz-15 (L15) medium (AL15) w/ pen-st..."
- 07:55, 6 August 2014 diff hist -1,895 Passaging A1 cell Blanked the page
- 07:53, 6 August 2014 diff hist +1,895 N A1 cell passaging myotube Created page with "<h4>For passaging 3 flasks of A1 cells:</h4> <li>1 flask into 3 flasks and <li>2 flasks into 2 x 10 cm plates (for making myotubes) <h3>Materials :</h3> <li>HS AMEM (see add..."
- 11:35, 4 August 2014 diff hist -1 Thawing (XB10) hybridoma cells
- 11:33, 4 August 2014 diff hist +1,150 Thawing (XB10) hybridoma cells
- 11:30, 4 August 2014 diff hist +15 Thawing (XB10) hybridoma cells
- 11:29, 4 August 2014 diff hist +427 Thawing (XB10) hybridoma cells
- 11:25, 4 August 2014 diff hist +14 N Thawing (XB10) hybridoma cells Created page with "==Materials:=="
- 11:23, 4 August 2014 diff hist +718 Hybridoma cells in SFX media
- 11:16, 4 August 2014 diff hist +201 N Hybridoma cells in SFX media Created page with "==Materials:== <li>5 ml, 10 ml, 20 ml pipettes <li>Serum free media( 400 mls SF media + 1% antibiotics 4 mls P/S and 4 mls Glutamine <li>2 blue cap..."
- 10:34, 2 July 2014 diff hist -1 Passaging A1 cell
- 10:33, 2 July 2014 diff hist -21 Passaging A1 cell
- 10:23, 2 July 2014 diff hist +21 Passaging A1 cell
- 09:12, 2 July 2014 diff hist -26 Passaging A1 cell
- 09:07, 2 July 2014 diff hist -5 Passaging A1 cell
- 09:04, 2 July 2014 diff hist +32 Passaging A1 cell
- 08:58, 2 July 2014 diff hist +9 Passaging A1 cell
- 08:57, 2 July 2014 diff hist +44 Passaging A1 cell
- 08:55, 2 July 2014 diff hist -86 Passaging A1 cell
- 08:52, 2 July 2014 diff hist +8 Passaging A1 cell
- 08:49, 2 July 2014 diff hist +9 Passaging A1 cell
- 08:41, 2 July 2014 diff hist +1 Passaging A1 cell
- 08:38, 2 July 2014 diff hist +1,910 N Passaging A1 cell Created page with "For passaging 3 flasks of A1 cells: 1 flask into 3 flasks and 2 flasks into 2 x10 cm plates (for making myotubes) Materials : HS AMEM (see additional protocol for recipe) ..."
- 09:34, 20 June 2014 diff hist +42 Letter to Froppier (French)
- 09:32, 20 June 2014 diff hist -42 Letter to Froppier (French)
- 09:30, 20 June 2014 diff hist +70 Letter to Froppier (French)
- 09:12, 20 June 2014 diff hist -9 Letter to Froppier (French)
- 09:08, 20 June 2014 diff hist -2 Letter to Froppier (French)
- 10:01, 18 June 2014 diff hist 0 Letter to Froppier (French)
- 10:00, 18 June 2014 diff hist 0 Letter to Froppier (French)
- 10:00, 18 June 2014 diff hist +1 Letter to Froppier (French)
- 09:57, 18 June 2014 diff hist +2 Letter to Froppier (French)
- 09:56, 18 June 2014 diff hist -8 Letter to Froppier (French)
- 09:56, 18 June 2014 diff hist +43 Letter to Froppier (French)
- 09:55, 18 June 2014 diff hist +20 Letter to Froppier (French)
- 09:53, 18 June 2014 diff hist +30 Letter to Froppier (French)
- 09:50, 18 June 2014 diff hist 0 Letter to Froppier (French)
- 09:49, 18 June 2014 diff hist +53 Letter to Froppier (French)
- 09:44, 18 June 2014 diff hist +3 Letter to Froppier (French)
- 09:42, 18 June 2014 diff hist -1 Letter to Froppier (French)
- 09:41, 18 June 2014 diff hist +89 Letter to Froppier (French)
- 09:30, 18 June 2014 diff hist +35 Letter to Froppier (French)
- 09:19, 18 June 2014 diff hist +733 N Letter to Froppier (French) Created page with "Max-Planck Institute Of Molecular Cell Biology and Genetics Pfotenhauerstrasse 108 01307 Dresden GERMANY G. FROPPIER Responsable Scientifique Elevage Scientifique des Dombes ..."
- 08:20, 11 June 2014 diff hist -2 BrdU and myosin staining →Materials:
- 08:19, 11 June 2014 diff hist -4 BrdU and myosin staining →Materials:
- 08:18, 11 June 2014 diff hist -6 BrdU and myosin staining →staining cells:
- 08:17, 11 June 2014 diff hist +20 BrdU and myosin staining →For staining cells:
- 08:15, 11 June 2014 diff hist +19 BrdU and myosin staining →Materials:
- 08:09, 11 June 2014 diff hist -15 BrdU and myosin staining
- 08:01, 11 June 2014 diff hist -14 BrdU and myosin staining
- 07:59, 11 June 2014 diff hist -7 BrdU and myosin staining
- 07:56, 11 June 2014 diff hist -2 BrdU and myosin staining
- 07:55, 11 June 2014 diff hist +3,204 N BrdU and myosin staining Created page with "== Fixing Cells: == 1 thaw 6% PFA in 37 C waterbath , place on ice 2 put 5 ml PBS + 5 ml 6% PFA in tray “PFA” , mix well 3 put 0.01% BSA/PBS into tray “BSA” ..."
- 08:27, 7 May 2014 diff hist +943 N Antibody staining Created page with "* Cut the tail and fix it in fresh 4% PFA in phosphate buffer from few hours to o/n always at 4C * Wash in PBS 1 hour at room temperature, put tail in 30% sucrose Put in OCT s..."
- 09:00, 2 May 2014 diff hist +252 BrdU labelling of A1 cells in 96-well plate
- 08:56, 2 May 2014 diff hist +102 N BrdU labelling of A1 cells in 96-well plate Created page with "== Materials: == * 5ml tube * SF MEM * BrDU drawer no. 3 * Multipipette * Pipette 100ul * Pipette 5ml"
- 08:46, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:45, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:36, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:34, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:32, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:29, 28 April 2014 diff hist +805 Passaging C2C12 cells
- 08:28, 28 April 2014 diff hist +17 Passaging C2C12 cells
- 06:58, 28 April 2014 diff hist +143 N Passaging C2C12 cells Created page with "== Materials: == * 1x 15ml FALCON tube * PBS w/o Ca2+ and Mg2+ * TE in PBS (4 ml TE + 36 ml PBS) * HS for C2C12 cells * pipettes * 75 cm2 flask"
- 09:32, 24 March 2014 diff hist +768 Purifying IgG1 from Hybridoma Supernatant
- 09:27, 24 March 2014 diff hist -45 Purifying IgG1 from Hybridoma Supernatant
- 09:25, 24 March 2014 diff hist +313 N Purifying IgG1 from Hybridoma Supernatant Created page with "<h3>Materials:</h3> <li>Supernatant (100-200 ml)</li> <li>Peristaltic pump</li> <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml)</li> <li>PBS (100 ml)</li> <li>10x ..."
- 12:48, 18 March 2014 diff hist -6 Overview →Protocols
- 12:45, 18 March 2014 diff hist +6 Overview →Antibody
- 12:42, 18 March 2014 diff hist +133 Overview →Protocols
- 12:19, 18 March 2014 diff hist +861 Labeled AB staining
- 12:12, 18 March 2014 diff hist +315 Labeled AB staining
- 12:06, 18 March 2014 diff hist +38 N Labeled AB staining Created page with "<h4>Pax7 + cy3 + NeuN – cy5Fab</h4>"
- 11:56, 18 March 2014 diff hist +50 Overview →Protocols
- 12:21, 10 March 2014 diff hist +10 RNA formaldehyde gels
- 12:17, 10 March 2014 diff hist +649 RNA formaldehyde gels
- 12:11, 10 March 2014 diff hist +205 RNA formaldehyde gels
- 12:06, 10 March 2014 diff hist +5 RNA formaldehyde gels
- 12:05, 10 March 2014 diff hist +248 N RNA formaldehyde gels Created page with "<h3>Prepare gel box: <li>Clean gel box and combs etc with detergent, then dionized water. <li>Then incubate the gel box and components in 10% H2O2 (hydrogen peroxide, stored i..."
- 12:01, 10 March 2014 diff hist +57 Overview →Molecular biology
- 08:52, 10 March 2014 diff hist -45 RNA Extraction from axolotl tissue
- 08:49, 10 March 2014 diff hist +755 RNA Extraction from axolotl tissue
- 08:43, 10 March 2014 diff hist +41 RNA Extraction from axolotl tissue
- 08:39, 10 March 2014 diff hist +56 RNA Extraction from axolotl tissue
- 08:34, 10 March 2014 diff hist +239 RNA Extraction from axolotl tissue
- 08:20, 10 March 2014 diff hist -1 RNA Extraction from axolotl tissue
- 08:20, 10 March 2014 diff hist +8 RNA Extraction from axolotl tissue
- 08:18, 10 March 2014 diff hist +12 N RNA Extraction from axolotl tissue Created page with " Materials:"
- 12:36, 6 March 2014 diff hist -5 Overview →Molecular biology
- 12:27, 6 March 2014 diff hist -3 Overview →Molecular biology
- 12:22, 6 March 2014 diff hist +89 Overview →Molecular biology
- 12:10, 6 March 2014 diff hist -102 Overview →RNA
- 09:30, 6 March 2014 diff hist +93 RNA extraction from axolotl tissue →Procedure to extract total RNA: current
- 09:21, 6 March 2014 diff hist +12 RNA extraction from axolotl tissue →Procedure to extract total RNA:
- 09:20, 6 March 2014 diff hist +1,242 N RNA extraction from axolotl tissue Created page with "== RNA Extraction from axolotl tissue == === Materials: === <ul> <li>Trizol (Invitrogen)</li> <li>Chloroform</li> <li>Isopropanol</li> <li>RNase free water</li> <..."
- 08:35, 6 March 2014 diff hist +95 Overview →Protocols