User contributions for Benjamin.wachtel
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26 July 2018
- 13:1713:17, 26 July 2018 diff hist −24 Gelatin Embedding →Gelatin Embedding current
- 13:1713:17, 26 July 2018 diff hist +732 N Gelatin Embedding Created page with "== Gelatin Embedding == #Fixation in 1% MEMFA (1m FA, 4ml 10x MEM, 35 ml H2O) #Wash 3x for 10’ each in 1x PBS #Infiltrate tissue with 10% Sucrose in 1x PBS @ 4°C for 1 hou..."
- 13:1213:12, 26 July 2018 diff hist +104 Overview →Cell culture
- 12:4312:43, 26 July 2018 diff hist +1,117 N Cocktail Protocol for Pax7 Created page with "Cocktail Protocol for Pax7 Cocktail Protocol for Pax7/Cy3 and NeuN/Cy5Fab *10µl NeuN *+ 11.8µl Cy5 *+ 80µl PBS/BSA (0.01%) *30’@RT in alufoil *+ 12µl α-BrdU *30’@RT..." current
- 12:4012:40, 26 July 2018 diff hist +67 Overview →Molecular biology
- 12:3012:30, 26 July 2018 diff hist +807 N RAMP Test Created page with "How To Run a RAMP TEST Sutter Micropipette Puller P-97 # Fo into any program. For example press 1 and ENTR to select Program 1 # Press CTRL # Press 0 to not clear parameters..."
- 12:2612:26, 26 July 2018 diff hist +33 Overview →Hardware
- 12:1612:16, 26 July 2018 diff hist +2,673 GRNA Production No edit summary current
20 July 2018
- 13:4013:40, 20 July 2018 diff hist +187 GRNA Production No edit summary
- 13:3813:38, 20 July 2018 diff hist +5,080 GRNA Production No edit summary
- 13:0013:00, 20 July 2018 diff hist +1,791 GRNA Production No edit summary
- 12:3212:32, 20 July 2018 diff hist +596 N GRNA Production Created page with "# Oligo Rehydration 100 μmol [ ] (stock) of oligos Ex. 100 μmol [ ] —> 27.8 nmol [ ] —> rehydrate oligo bead with 278 μl of H2O *add H2O to hydrate oligos *incubate 5 m..."
- 12:2912:29, 20 July 2018 diff hist +45 Overview →Molecular biology
- 12:2712:27, 20 July 2018 diff hist +1,451 N Linker Insertion Created page with "Oligo Kinasing and Annealing From Regis / Mike 1. Kinasing oligos - Incubate 30 min at 37° C #10 µl oligo (50pmoles/Microliter) #5 µl h2O #2 µl 10x kinase buffer #2 µ..." current
- 12:2112:21, 20 July 2018 diff hist +47 Overview →Molecular biology
- 12:1812:18, 20 July 2018 diff hist +1,024 N Amputation andRNA Isolation Created page with "RNA Isolation *Axolotl: 0.01% Bencocain depends of the size!! *Wait ca. 10min before cutting! *Collect the tissue in ca 0,5 ml fluid nitrogen and put into -80° Homogenisatio..." current
- 12:1512:15, 20 July 2018 diff hist +70 Overview →Molecular biology
19 July 2018
- 12:3112:31, 19 July 2018 diff hist −45 Overview →A1 cells
- 12:2812:28, 19 July 2018 diff hist +45 Overview →A1 cells
- 12:0912:09, 19 July 2018 diff hist 0 Insulin Preparation No edit summary current
- 12:0812:08, 19 July 2018 diff hist +1,196 N Insulin Preparation Created page with "Media for passaging A1 cells. #For 400 mls #FCS 40 ml #Eagle's MEM 252 ml #DdH2O 100 ml #100xPen/Strep 2 ml #100x Glutamine 2 ml #Insulin (stock 1 mg/ml) 2 m..."
- 12:0112:01, 19 July 2018 diff hist +53 Overview →Cell culture
- 11:4011:40, 19 July 2018 diff hist +618 Axolotl →Maintaining the axolotls
- 11:2711:27, 19 July 2018 diff hist +2,690 Axolotl →Genotyping
- 11:2311:23, 19 July 2018 diff hist +18 Axolotl No edit summary
- 11:2111:21, 19 July 2018 diff hist +141 Overview →Axolotl
11 July 2018
- 14:2114:21, 11 July 2018 diff hist +3,656 N Antibody purification Sox2 Created page with "Exchange the buffer of the MBP fusion protein using PD10 columns *Equilibration buffer #100 mM HEPES pH 8.0 #500 mM NaCl #10 mM Maltose #Add CaCl2 to 80 mM final conc. #Save..." current
- 14:1014:10, 11 July 2018 diff hist +40 Overview →Polyclonal Antibodies:
- 14:0414:04, 11 July 2018 diff hist +2,669 Antibody purification No edit summary current
- 13:5913:59, 11 July 2018 diff hist +1,318 N Antibody purification Created page with " Exchange the buffer of the MBP fusion protein using pD10 columns *Equilibration buffer: 100 mM HEPES pH 8.0 (shelf!) 500 mM NaCl (2.922g NaCl for 100 ml; M=58.44g/mol) 10..."
- 13:5613:56, 11 July 2018 diff hist +35 Overview →Polyclonal Antibodies:
- 13:5213:52, 11 July 2018 diff hist +577 N Gelatin Preparation Created page with "Materials: *Gelatin Type A Porcine skin, 300 bloom (SIGMA, Cat#: G-2500); 7.5g *ddH2O; 1L *50ml Conical tube (Corning, Cat#: 340829); 20 *Filter bottle (Corning, Cat#: 431153)..." current
- 13:4913:49, 11 July 2018 diff hist +54 Overview No edit summary
- 13:4713:47, 11 July 2018 diff hist 0 Overview No edit summary
- 13:4613:46, 11 July 2018 diff hist +701 N Fibronectin preparation Created page with "Materials; *Fibronectin from bovine plasma (SIGMA, Cat#: F4759-5MG); 5mg ddH2O; 5ml *Filter for syringe (0.22 µm); 1 *Syringe (10ml or bigger) *15ml Conical tube (Corning, Ca..." current
- 13:4413:44, 11 July 2018 diff hist +60 Overview →Cell culture
- 13:4213:42, 11 July 2018 diff hist −56 Overview No edit summary
- 13:4113:41, 11 July 2018 diff hist +57 Overview →Cell culture
- 13:3013:30, 11 July 2018 diff hist +1,321 Axolotl No edit summary
- 13:2613:26, 11 July 2018 diff hist +157 Overview No edit summary
- 13:2213:22, 11 July 2018 diff hist +532 Axolotl No edit summary