User contributions
- 13:17, 26 July 2018 (diff | hist) . . (-24) . . Gelatin Embedding (→Gelatin Embedding) (current)
- 13:17, 26 July 2018 (diff | hist) . . (+732) . . N Gelatin Embedding (Created page with "== Gelatin Embedding == #Fixation in 1% MEMFA (1m FA, 4ml 10x MEM, 35 ml H2O) #Wash 3x for 10’ each in 1x PBS #Infiltrate tissue with 10% Sucrose in 1x PBS @ 4°C for 1 hou...")
- 13:12, 26 July 2018 (diff | hist) . . (+104) . . Overview (→Cell culture)
- 12:43, 26 July 2018 (diff | hist) . . (+1,117) . . N Cocktail Protocol for Pax7 (Created page with "Cocktail Protocol for Pax7 Cocktail Protocol for Pax7/Cy3 and NeuN/Cy5Fab *10µl NeuN *+ 11.8µl Cy5 *+ 80µl PBS/BSA (0.01%) *30’@RT in alufoil *+ 12µl α-BrdU *30’@RT...") (current)
- 12:40, 26 July 2018 (diff | hist) . . (+67) . . Overview (→Molecular biology)
- 12:30, 26 July 2018 (diff | hist) . . (+807) . . N RAMP Test (Created page with "How To Run a RAMP TEST Sutter Micropipette Puller P-97 # Fo into any program. For example press 1 and ENTR to select Program 1 # Press CTRL # Press 0 to not clear parameters...")
- 12:26, 26 July 2018 (diff | hist) . . (+33) . . Overview (→Hardware)
- 12:16, 26 July 2018 (diff | hist) . . (+2,673) . . GRNA Production (current)
- 13:40, 20 July 2018 (diff | hist) . . (+187) . . GRNA Production
- 13:38, 20 July 2018 (diff | hist) . . (+5,080) . . GRNA Production
- 13:00, 20 July 2018 (diff | hist) . . (+1,791) . . GRNA Production
- 12:32, 20 July 2018 (diff | hist) . . (+596) . . N GRNA Production (Created page with "# Oligo Rehydration 100 μmol [ ] (stock) of oligos Ex. 100 μmol [ ] —> 27.8 nmol [ ] —> rehydrate oligo bead with 278 μl of H2O *add H2O to hydrate oligos *incubate 5 m...")
- 12:29, 20 July 2018 (diff | hist) . . (+45) . . Overview (→Molecular biology)
- 12:27, 20 July 2018 (diff | hist) . . (+1,451) . . N Linker Insertion (Created page with "Oligo Kinasing and Annealing From Regis / Mike 1. Kinasing oligos - Incubate 30 min at 37° C #10 µl oligo (50pmoles/Microliter) #5 µl h2O #2 µl 10x kinase buffer #2 µ...") (current)
- 12:21, 20 July 2018 (diff | hist) . . (+47) . . Overview (→Molecular biology)
- 12:18, 20 July 2018 (diff | hist) . . (+1,024) . . N Amputation andRNA Isolation (Created page with "RNA Isolation *Axolotl: 0.01% Bencocain depends of the size!! *Wait ca. 10min before cutting! *Collect the tissue in ca 0,5 ml fluid nitrogen and put into -80° Homogenisatio...") (current)
- 12:15, 20 July 2018 (diff | hist) . . (+70) . . Overview (→Molecular biology)
- 12:31, 19 July 2018 (diff | hist) . . (-45) . . Overview (→A1 cells)
- 12:28, 19 July 2018 (diff | hist) . . (+45) . . Overview (→A1 cells)
- 12:09, 19 July 2018 (diff | hist) . . (0) . . Insulin Preparation (current)
- 12:08, 19 July 2018 (diff | hist) . . (+1,196) . . N Insulin Preparation (Created page with "Media for passaging A1 cells. #For 400 mls #FCS 40 ml #Eagle's MEM 252 ml #DdH2O 100 ml #100xPen/Strep 2 ml #100x Glutamine 2 ml #Insulin (stock 1 mg/ml) 2 m...")
- 12:01, 19 July 2018 (diff | hist) . . (+53) . . Overview (→Cell culture)
- 11:40, 19 July 2018 (diff | hist) . . (+618) . . Axolotl (→Maintaining the axolotls)
- 11:27, 19 July 2018 (diff | hist) . . (+2,690) . . Axolotl (→Genotyping)
- 11:23, 19 July 2018 (diff | hist) . . (+18) . . Axolotl
- 11:21, 19 July 2018 (diff | hist) . . (+141) . . Overview (→Axolotl)
- 14:21, 11 July 2018 (diff | hist) . . (+3,656) . . N Antibody purification Sox2 (Created page with "Exchange the buffer of the MBP fusion protein using PD10 columns *Equilibration buffer #100 mM HEPES pH 8.0 #500 mM NaCl #10 mM Maltose #Add CaCl2 to 80 mM final conc. #Save...") (current)
- 14:10, 11 July 2018 (diff | hist) . . (+40) . . Overview (→Polyclonal Antibodies:)
- 14:04, 11 July 2018 (diff | hist) . . (+2,669) . . Antibody purification (current)
- 13:59, 11 July 2018 (diff | hist) . . (+1,318) . . N Antibody purification (Created page with " Exchange the buffer of the MBP fusion protein using pD10 columns *Equilibration buffer: 100 mM HEPES pH 8.0 (shelf!) 500 mM NaCl (2.922g NaCl for 100 ml; M=58.44g/mol) 10...")
- 13:56, 11 July 2018 (diff | hist) . . (+35) . . Overview (→Polyclonal Antibodies:)
- 13:52, 11 July 2018 (diff | hist) . . (+577) . . N Gelatin Preparation (Created page with "Materials: *Gelatin Type A Porcine skin, 300 bloom (SIGMA, Cat#: G-2500); 7.5g *ddH2O; 1L *50ml Conical tube (Corning, Cat#: 340829); 20 *Filter bottle (Corning, Cat#: 431153)...") (current)
- 13:49, 11 July 2018 (diff | hist) . . (+54) . . Overview
- 13:47, 11 July 2018 (diff | hist) . . (0) . . Overview
- 13:46, 11 July 2018 (diff | hist) . . (+701) . . N Fibronectin preparation (Created page with "Materials; *Fibronectin from bovine plasma (SIGMA, Cat#: F4759-5MG); 5mg ddH2O; 5ml *Filter for syringe (0.22 µm); 1 *Syringe (10ml or bigger) *15ml Conical tube (Corning, Ca...") (current)
- 13:44, 11 July 2018 (diff | hist) . . (+60) . . Overview (→Cell culture)
- 13:42, 11 July 2018 (diff | hist) . . (-56) . . Overview
- 13:41, 11 July 2018 (diff | hist) . . (+57) . . Overview (→Cell culture)
- 13:30, 11 July 2018 (diff | hist) . . (+1,321) . . Axolotl
- 13:26, 11 July 2018 (diff | hist) . . (+157) . . Overview
- 13:22, 11 July 2018 (diff | hist) . . (+532) . . Axolotl
