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- 15:37, 19 July 2022 diff hist -436 Overview →Protocols current
- 23:55, 18 July 2022 diff hist -86 Frog Prrx1:CreER line screening current
- 23:52, 18 July 2022 diff hist +3,598 N Frog Prrx1:CreER line screening Created page with "Transgenic TLM115 Frog Screening Protocol 2022.07.18 Tzi-Yang A. Mating (ask Birgit if not clear), ~1 hour 1. Choose the mating pairs and make notes on the frog list. • E...."
- 23:51, 18 July 2022 diff hist +81 Overview →Protocols
- 23:49, 18 July 2022 diff hist +42 For cell transplantation →Cell transplantation current
- 23:48, 18 July 2022 diff hist 0 N File:Text1-1.png current
- 23:47, 18 July 2022 diff hist 0 N File:Text1-2.png current
- 23:42, 18 July 2022 diff hist 0 N File:Text3.png current
- 23:42, 18 July 2022 diff hist 0 N File:Text2.png current
- 23:41, 18 July 2022 diff hist +85 For cell transplantation →Cell transplantation
- 23:37, 18 July 2022 diff hist +53 For cell transplantation →Cell transplantation
- 23:24, 18 July 2022 diff hist +33 For cell transplantation →Cell transplantation
- 23:23, 18 July 2022 diff hist +56 For cell transplantation →Cell transplantation
- 23:21, 18 July 2022 diff hist 0 N File:3.png current
- 23:21, 18 July 2022 diff hist 0 N File:2.png current
- 23:20, 18 July 2022 diff hist 0 File:Screenshot 2022-07-19 at 01.18.01.png Tzi.lin uploaded a new version of File:Screenshot 2022-07-19 at 01.18.01.png current
- 23:18, 18 July 2022 diff hist 0 N File:Screenshot 2022-07-19 at 01.18.01.png
- 23:09, 18 July 2022 diff hist +902 N For cell transplantation Created page with "= Cell transplantation = ---- <li>(Continuing directly from Liberase dissociation or from FACS)</li> *Resuspend the cell..."
- 22:58, 18 July 2022 diff hist +39 For scRNA-seq →scRNA-seq current
- 22:57, 18 July 2022 diff hist +121 For scRNA-seq →scRNA-seq
- 22:48, 18 July 2022 diff hist 0 LiberaseTM cell dissociation protocol →Liberase TM cell dissociation on amphibian limb tissue current
- 22:48, 18 July 2022 diff hist -4 For scRNA-seq →scRNA-seq
- 22:47, 18 July 2022 diff hist +38 For scRNA-seq →scRNA-seq
- 22:45, 18 July 2022 diff hist +24 LiberaseTM cell dissociation protocol →Liberase TM cell dissociation on amphibian limb tissue
- 22:44, 18 July 2022 diff hist +191 LiberaseTM cell dissociation protocol →Liberase TM cell dissociation on amphibian limb tissue
- 22:40, 18 July 2022 diff hist +45 LiberaseTM cell dissociation protocol →Liberase TM cell dissociation on amphibian limb tissue
- 22:39, 18 July 2022 diff hist -29 For scRNA-seq →scRNA-seq
- 22:37, 18 July 2022 diff hist +41 For FACS →FACS current
- 22:35, 18 July 2022 diff hist 0 LiberaseTM cell dissociation protocol →Liberase TM cell dissociation on amphibian limb tissue
- 22:34, 18 July 2022 diff hist +34 LiberaseTM cell dissociation protocol →Liberase TM cell dissociation on amphibian limb tissue
- 22:33, 18 July 2022 diff hist +2,425 N LiberaseTM cell dissociation protocol Created page with "= Prepare Reagents = ---- === 0.7x PBS === *Dilute 1x PBS to 0.7x with dH2O :-> Chill on ice before use. === Liberase TM solution === *40uL Stock Liberase TM (100x, 26WU/mL)..."
- 22:29, 18 July 2022 diff hist +20 For FACS →FACS
- 22:29, 18 July 2022 diff hist 0 Overview →LiberaseTM cell dissociation protocol
- 22:28, 18 July 2022 diff hist -19 Overview →LiberaseTM cell dissociation for axolotl/frog limb cells
- 22:27, 18 July 2022 diff hist +65 LiberaseTM cell dissociation for axolotl/frog limb cells →Liberase TM cell dissociation on amphibian limb tissue current
- 22:23, 18 July 2022 diff hist +551 N For scRNA-seq Created page with "= scRNA-seq = ---- <li>(Continue from the [[|dissociation protocol]])</li> *After 30um filter cup, immediately filter through 10um filter cup Resuspend the cell pellet with 15..."
- 22:21, 18 July 2022 diff hist +11 LiberaseTM cell dissociation for axolotl/frog limb cells →Liberase TM cell dissociation on amphibian limb tissue
- 22:19, 18 July 2022 diff hist -12 LiberaseTM cell dissociation for axolotl/frog limb cells →Liberase TM cell dissociation on amphibian limb tissue
- 22:18, 18 July 2022 diff hist -11 LiberaseTM cell dissociation for axolotl/frog limb cells →Liberase TM cell dissociation on amphibian limb tissue
- 22:14, 18 July 2022 diff hist +476 N For FACS Created page with "= FACS = ---- <li>(Continue from the dissociation protocol)</li> *Resuspend the cell pellet with 150-300uL AMEM(0) depending on the size of the pellet. *Immediately sort the c..."
- 22:08, 18 July 2022 diff hist -5 LiberaseTM cell dissociation for axolotl/frog limb cells →Liberase TM cell dissociation on amphibian limb tissue
- 22:08, 18 July 2022 diff hist 0 LiberaseTM cell dissociation for axolotl/frog limb cells →Liberase TM cell dissociation on amphibian limb tissue
- 22:08, 18 July 2022 diff hist +29 LiberaseTM cell dissociation for axolotl/frog limb cells →Liberase TM cell dissociation on amphibian limb tissue
- 22:08, 18 July 2022 diff hist +1 Overview →Downstream application: FACS
- 22:07, 18 July 2022 diff hist +3 LiberaseTM cell dissociation for axolotl/frog limb cells →Liberase TM cell dissociation on amphibian limb tissue
- 22:06, 18 July 2022 diff hist +3 LiberaseTM cell dissociation for axolotl/frog limb cells
- 22:06, 18 July 2022 diff hist +1,544 LiberaseTM cell dissociation for axolotl/frog limb cells
- 21:48, 18 July 2022 diff hist +697 LiberaseTM cell dissociation for axolotl/frog limb cells →Prepare Reagents
- 21:40, 18 July 2022 diff hist +113 N LiberaseTM cell dissociation for axolotl/frog limb cells Created page with "= Prepare Reagents = ---- ‘’’0.7x PBS’’’ *Dilute 1x PBS to 0.7x with dH2O :-> Chill on ice before use"
- 21:37, 18 July 2022 diff hist -3 Overview →LiberaseTM cell dissociation for axolotl/frog limb cells
- 21:35, 18 July 2022 diff hist -50 Overview →Cell dissociation and downstream applications
- 21:32, 18 July 2022 diff hist +135 Overview →Protocols
- 21:27, 18 July 2022 diff hist +8 Overview →Cell dissociation and downstream applications
- 21:26, 18 July 2022 diff hist +8 Overview →Cell dissociation and downstream applications
- 21:24, 18 July 2022 diff hist -9 Overview →Cell dissociation and downstream applications
- 21:22, 18 July 2022 diff hist +1 LiberaseTM dissociation →Liberase TM dissociation current
- 21:21, 18 July 2022 diff hist -4 LiberaseTM dissociation →Liberase TM dissociation
- 21:16, 18 July 2022 diff hist 0 LiberaseTM dissociation →Liberase TM dissociation
- 21:16, 18 July 2022 diff hist 0 LiberaseTM dissociation →Liberase TM dissociation
- 21:16, 18 July 2022 diff hist +4 LiberaseTM dissociation →Liberase TM dissociation
- 21:15, 18 July 2022 diff hist -9 LiberaseTM dissociation →Liberase TM dissociation
- 21:12, 18 July 2022 diff hist +55 LiberaseTM dissociation →Liberase TM dissociation
- 21:10, 18 July 2022 diff hist -3 With FACS →For FACS current
- 21:10, 18 July 2022 diff hist +131 With FACS →For FACS
- 21:07, 18 July 2022 diff hist +495 N With FACS Created page with "= For FACS = ---- *Finish the dissociation protocol *Resuspend in 150-200uL AMEM(0) *Send to FACS facility immediately **Book FACS machine F02 because it has both GFP and mChe..."
- 21:00, 18 July 2022 diff hist +1 Overview →Cell dissociation and downstream applications
- 21:00, 18 July 2022 diff hist -1 Overview →Cell dissociation and downstream applications
- 20:59, 18 July 2022 diff hist +1 Overview →Cell dissociation and downstream applications
- 20:59, 18 July 2022 diff hist +2 Overview →Cell dissociation and downstream applications
- 20:58, 18 July 2022 diff hist +133 Overview →Cell dissociation and downstream applications
- 20:56, 18 July 2022 diff hist +27 Overview →Cell dissociation
- 20:54, 18 July 2022 diff hist +846 LiberaseTM dissociation →Liberase TM dissociation
- 20:45, 18 July 2022 diff hist -4,788 LiberaseTM dissociation →Liberase TM dissociation
- 20:42, 18 July 2022 diff hist +278 LiberaseTM dissociation →Preparing reagents
- 20:39, 18 July 2022 diff hist +271 LiberaseTM dissociation →Preparing reagents
- 20:36, 18 July 2022 diff hist -2 LiberaseTM dissociation →Liberase TM solution
- 20:36, 18 July 2022 diff hist +47 LiberaseTM dissociation →Preparing reagents
- 20:24, 18 July 2022 diff hist +7 LiberaseTM dissociation →Preparing reagents
- 20:23, 18 July 2022 diff hist +5,469 LiberaseTM dissociation →Preparing reagents
- 20:20, 18 July 2022 diff hist -1 LiberaseTM dissociation
- 20:19, 18 July 2022 diff hist +2 LiberaseTM dissociation
- 20:19, 18 July 2022 diff hist -2 LiberaseTM dissociation →Preparing reagents
- 20:18, 18 July 2022 diff hist +9 LiberaseTM dissociation →Preparing reagents
- 20:17, 18 July 2022 diff hist +257 LiberaseTM dissociation →Preparing reagents
- 20:12, 18 July 2022 diff hist -1 LiberaseTM dissociation →Preparing reagents
- 20:11, 18 July 2022 diff hist +6 LiberaseTM dissociation →Preparing reagents
- 20:11, 18 July 2022 diff hist -4 LiberaseTM dissociation →Preparing reagents
- 20:11, 18 July 2022 diff hist -2 LiberaseTM dissociation →Preparing reagents
- 20:11, 18 July 2022 diff hist +28 N LiberaseTM dissociation Created page with "==== Preparing reagents ===="
- 20:06, 18 July 2022 diff hist +6 Overview →Cell dissociation
- 20:06, 18 July 2022 diff hist +27 Overview →Cell dissociation
- 20:03, 18 July 2022 diff hist +88 Overview →Protocols