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- 11:27, 23 March 2015 diff hist -1 A1 cell transfection with FuGene
- 11:27, 23 March 2015 diff hist +2 A1 cell transfection with FuGene
- 11:21, 23 March 2015 diff hist +1,463 N A1 cell transfection with FuGene Created page with "== Materials == * Fugene Transfection solution * Complete media for A1 cells * No serum media * ATE * APBS * 10 cm dishes * eppendorf tubes == Procedure == Incubation FuGen..."
- 08:47, 11 March 2015 diff hist +14 Cardiomyocyte Preparation
- 08:46, 11 March 2015 diff hist +1 Cardiomyocyte Preparation
- 08:44, 11 March 2015 diff hist -15 Cardiomyocyte Preparation
- 08:41, 11 March 2015 diff hist +4 Cardiomyocyte Preparation
- 08:40, 11 March 2015 diff hist +12 Cardiomyocyte Preparation
- 08:38, 11 March 2015 diff hist 0 Preparation of Low Serum for C2C12 cells
- 08:36, 11 March 2015 diff hist 0 Thawing 293FT cells
- 08:34, 11 March 2015 diff hist +2 Preparation of Low Serum for C2C12 cells
- 08:32, 11 March 2015 diff hist 0 Preparation of Low Serum for C2C12 cells
- 08:31, 11 March 2015 diff hist -8 Preparation of Low Serum for C2C12 cells
- 08:30, 11 March 2015 diff hist +5 Preparation of Low Serum for C2C12 cells
- 08:30, 11 March 2015 diff hist +475 N Preparation of Low Serum for C2C12 cells Created page with "== materials == <li> 390 mls DMEM-1 tube peni./ strepto. <li> tube glutamine (no insulin) <li> 8 mls Horse serum-1 sterile bottle <li> 500 ml sterile cylinder sterile fi..."
- 08:28, 16 February 2015 diff hist -51 Labelling myosin antibody
- 08:20, 16 February 2015 diff hist -2 Labelling myosin antibody
- 08:18, 16 February 2015 diff hist +20 Labelling myosin antibody
- 08:14, 16 February 2015 diff hist +4 Labelling myosin antibody
- 08:14, 16 February 2015 diff hist +36 Labelling myosin antibody
- 08:10, 16 February 2015 diff hist +978 N Labelling myosin antibody Created page with "0.2 M Borate buffer, pH 8 Biorad protein assay substance IgG standard Anti myosin antibody (4A1025) FlNHS (1.5 mg/ml in DMSO—make up fresh just before use) 100mM glycine, pH..."
- 10:25, 13 February 2015 diff hist -2 Preparation of High Serum for A1 cells
- 10:25, 13 February 2015 diff hist +3 Preparation of High Serum for A1 cells
- 10:24, 13 February 2015 diff hist +5 Preparation of High Serum for A1 cells
- 10:22, 13 February 2015 diff hist -1 Preparation of High Serum for A1 cells
- 10:20, 13 February 2015 diff hist 0 Preparation of High Serum for A1 cells
- 10:19, 13 February 2015 diff hist +7 Preparation of High Serum for A1 cells
- 10:16, 13 February 2015 diff hist +5 Preparation of High Serum for A1 cells
- 10:15, 13 February 2015 diff hist +16 Preparation of High Serum for A1 cells
- 10:09, 13 February 2015 diff hist +4 Preparation of High Serum for A1 cells
- 10:07, 13 February 2015 diff hist +465 N Preparation of High Serum for A1 cells Created page with "For 400 ml FCS 40 ml Eagle’s MEM 252 ml DdH2O 100 ml Pen/ Strep 4 ml Glutamin 4 ml Insulin (stock: 1mg/ ml) 4 ml - grow at 25ºC in 2% CO2 - if you want to gr..."
- 08:40, 13 February 2015 diff hist -14 Alcian Blue Staining
- 08:38, 13 February 2015 diff hist +599 N Alcian Blue Staining Created page with "# Fix sample O/N in 4% PFA freshly made # Wash in PBS, 3X5mins, 3X30mins 3. Wash 20mins in PBST 4. Equilibrate in EtOH, 25, 50%, 10 mins each 5. Place in Alcian blue staini..."
- 08:42, 9 February 2015 diff hist -2 Myoblasts electroporation
- 08:36, 9 February 2015 diff hist +6 Myoblasts electroporation
- 08:35, 9 February 2015 diff hist +4 Myoblasts electroporation
- 08:30, 9 February 2015 diff hist -9 Letter to Froppier (French) current
- 09:29, 2 February 2015 diff hist +265 Letter to Froppier (French)
- 13:00, 12 January 2015 diff hist +16 Freezing of A1 cells
- 12:54, 12 January 2015 diff hist +2 Electroporation of A1 cells
- 12:38, 12 January 2015 diff hist +2 Cloning of C2C12 cells
- 12:37, 12 January 2015 diff hist -7 Cloning of C2C12 cells
- 12:32, 12 January 2015 diff hist +7 Cloning of C2C12 cells
- 12:29, 12 January 2015 diff hist +4 Cloning of C2C12 cells
- 12:28, 12 January 2015 diff hist +2,085 Cloning of C2C12 cells
- 12:24, 12 January 2015 diff hist +1 N Cloning of C2C12 cells Created page with "#"
- 08:40, 12 January 2015 diff hist -1 Freezing of A1 cells
- 08:35, 12 January 2015 diff hist -1 Myoblasts electroporation
- 08:30, 12 January 2015 diff hist +654 N Freezing of A1 cells Created page with "''in one 175 cm2 flask'' Materials: - APBS (100 ml PBS + 25 ml diwater) - TE in APBS (36 ml APBS + 4 ml TE) - 1x 15 ml FALCON tubes - Pipettes - 1 cryo tube - freezing media ..."
- 12:58, 3 November 2014 diff hist -4 C2C12 cell transfection with FuGene