Difference between revisions of "Preparation of Freezing media for C2C12 cells"
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(Created page with "Materials: 25 ml FCS (50%) 20 ml DMEM + Pen/ Strep + Glut (40%) 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro Procedure: - put all stuffs together - filtate the mediu...") |
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Revision as of 08:50, 25 March 2015
Materials:
25 ml FCS (50%) 20 ml DMEM + Pen/ Strep + Glut (40%) 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro
Procedure:
- put all stuffs together - filtate the medium and aliquote the freezing media into 4 ml per tube - store at –20ºC