Difference between revisions of "For scRNA-seq"
Jump to navigation
Jump to search
(Continuing directly from Liberase dissociation or from FACS)
| Line 1: | Line 1: | ||
= scRNA-seq = | = scRNA-seq = | ||
---- | ---- | ||
| − | <li>(Continuing directly from | + | <li>(Continuing directly from [[LiberaseTM cell dissociation protocol|Liberase dissociation]] or from [[for FACS|FACS]])</li> |
*Resuspend the cell pellet with 150-300uL AMEM(0) depending on the size of the pellet. | *Resuspend the cell pellet with 150-300uL AMEM(0) depending on the size of the pellet. | ||
*Immediately sort the cells into 1.5mL tube with 700uL AMEM(0). | *Immediately sort the cells into 1.5mL tube with 700uL AMEM(0). | ||
Revision as of 22:48, 18 July 2022
scRNA-seq
- Resuspend the cell pellet with 150-300uL AMEM(0) depending on the size of the pellet.
- Immediately sort the cells into 1.5mL tube with 700uL AMEM(0).
- Normally we use F02 sorter because it has lasers for both GFP and mCherry.
- Note the FACS count.
- Centrifuge 300xrcf, 20 degree, 5 minutes. Remove supernatant.
- The sample is ready for downstream applications: