For FACS

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Revision as of 22:29, 18 July 2022 by Tzi.lin (talk | contribs) (→‎FACS)
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FACS


  • (Continue from the LiberaseTM cell dissociation protocol)
    • Resuspend the cell pellet with 150-300uL AMEM(0) depending on the size of the pellet.
    • Immediately sort the cells into 1.5mL tube with 700uL AMEM(0).
      • Normally we use F02 sorter because it has lasers for both GFP and mCherry.
    • Note the FACS count.
    • Centrifuge 300xrcf, 20 degree, 5 minutes. Remove supernatant.
    • The sample is ready for downstream applications:
    -> scRNA-seq
    -> cell transplantation