Difference between revisions of "Blastema cells"
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| − | == Composition for A1/BL cells == | + | == Composition of the medium for A1/BL cells == |
<ul> | <ul> | ||
<li>250 ml MEM 21090</li> | <li>250 ml MEM 21090</li> | ||
Latest revision as of 16:35, 16 January 2013
Composition of the medium for A1/BL cells
- 250 ml MEM 21090
- 100 ml H2O
- 40 ml FCS for A1 cells
- 4 ml pen/strep
- 4 ml insulin
- 4 ml glutamin
NOTE: Filter the medium after mixing!
PBS
Dilute normal PBS (w/o bivalent cations) in water (both are supplied - cupboard in the corridor in opposite of the cell culture).
The final PBS concentration must be 80%.
Trypsin
Dilute 10xTE > in the -20°C freezer in the cell culture preparation room (black labelling) with APBS.