Blastema cells

Composition for A1/BL cells

• 250 ml MEM 21090
• 100 ml H2O
• 40 ml FCS for A1 cells
• 4 ml pen/strep
• 4 ml insulin
• 4 ml glutamin

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NOTE: Filter the medium after mixing!

PBS

Dilute normal PBS (w/o bivalent cations) in water (both are supplied - cupboard in the corridor in opposite of the cell culture).
The final PBS concentration must be 80%.

Trypsin

Dilute 10xTE > in the -20°C freezer in the cell culture preparation room (black labelling) with APBS.