User contributions for Stephanh
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1 June 2015
- 08:1908:19, 1 June 2015 diff hist −10 TSA Staining on axolotl-tissue No edit summary
- 08:1808:18, 1 June 2015 diff hist +10 TSA Staining on axolotl-tissue No edit summary
- 08:1708:17, 1 June 2015 diff hist −14 TSA Staining on axolotl-tissue No edit summary
- 08:1408:14, 1 June 2015 diff hist −6 TSA Staining on axolotl-tissue No edit summary
- 08:1208:12, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue No edit summary
- 08:0608:06, 1 June 2015 diff hist +9 TSA Staining on axolotl-tissue No edit summary
- 07:5007:50, 1 June 2015 diff hist +1,096 N TSA Staining on axolotl-tissue Created page with "Solutions: - TNT wash buffer: 0.1M TRIS-HCL, pH 7.5 0.15M NaCl 0.05% Tween20 - TNB buffer: a) for blocking: 0.1M TRIS-HCL, pH 7.5 0.15M NaCl 20% goat serum b) for di..."
4 May 2015
- 14:1114:11, 4 May 2015 diff hist −16 MESC culture No edit summary
- 14:0614:06, 4 May 2015 diff hist 0 MESC culture No edit summary
- 10:0710:07, 4 May 2015 diff hist −11 MESC culture No edit summary
- 10:0610:06, 4 May 2015 diff hist −4 MESC culture No edit summary
- 10:0410:04, 4 May 2015 diff hist +19 MESC culture No edit summary
- 10:0210:02, 4 May 2015 diff hist +17 MESC culture No edit summary
- 08:4308:43, 4 May 2015 diff hist +4,160 N MESC culture Created page with "46C-ES cells/cyst formation Thawing of 46C ES cells: Prepare the growth medium: 405 ml DMEM + high glucose * Glutamax (Invitrogen: 31966) 75 ml FCS 5 ml L-Gln 100x (Invitro..."
17 April 2015
- 13:2013:20, 17 April 2015 diff hist 0 Electroporation of C2C12 cells No edit summary
- 13:2013:20, 17 April 2015 diff hist −1 Electroporation of C2C12 cells →material
- 13:1713:17, 17 April 2015 diff hist 0 Electroporation of C2C12 cells →material
- 13:1513:15, 17 April 2015 diff hist +1,228 N Electroporation of C2C12 cells Created page with "== material == Transfection * 1x Steinberger Medium * 1x DMEM without serum - remove the old media - wash the cells with 5 ml PBS - remove the PBS - add 2 ml TE - put the..."
- 12:5812:58, 17 April 2015 diff hist 0 Preparation of Freezing media for A1 cells →Procedure
- 12:5412:54, 17 April 2015 diff hist +11 Preparation of Freezing media for A1 cells No edit summary
- 12:5212:52, 17 April 2015 diff hist +432 N Preparation of Freezing media for A1 cells Created page with "Materials * DMSO (one ampules from Sigma) * High serum for A1 cells * 2x 50 ml FALCON tubes * 1x 15 ml FALCON tube * Pipettes ** 10x 5 ml FALCON tubes Procedure - clean the a..."
- 12:4512:45, 17 April 2015 diff hist 0 Preparation of Low Serum for A1 cells →Procedure
- 12:4412:44, 17 April 2015 diff hist 0 Preparation of Low Serum for A1 cells →Materials
- 12:4012:40, 17 April 2015 diff hist +261 N Preparation of Low Serum for A1 cells Created page with "== Materials == - 280 ml MEM - 100 ml sterile water - 1 tube Pen/ Strep (4ml) - 1 tube Glutamine (4ml) - 1 tube Insulin (4 ml) - 2 ml FCS - 500 ml filter with bottle == Proce..."
27 March 2015
- 10:5410:54, 27 March 2015 diff hist −22 RNA formaldehyde gels No edit summary current
- 10:5210:52, 27 March 2015 diff hist −3 RNA formaldehyde gels No edit summary
- 10:5110:51, 27 March 2015 diff hist +13 RNA formaldehyde gels No edit summary
- 10:4810:48, 27 March 2015 diff hist −17 RNA Extraction from axolotl tissue No edit summary
- 10:4510:45, 27 March 2015 diff hist −22 Digestion DNA →Digestion current
- 10:4410:44, 27 March 2015 diff hist −28 Digestion DNA No edit summary
- 10:4210:42, 27 March 2015 diff hist +8 Digestion DNA No edit summary
- 10:4010:40, 27 March 2015 diff hist +16 Metamorphosis protocol No edit summary current
- 10:3910:39, 27 March 2015 diff hist +16 Antibody staining No edit summary current
- 10:3610:36, 27 March 2015 diff hist +11 Labeled AB staining No edit summary
- 10:3310:33, 27 March 2015 diff hist +4 Labelling Pax6 with Dig-NHS No edit summary current
- 10:3210:32, 27 March 2015 diff hist −5 Labelling myosin antibody No edit summary
- 10:3110:31, 27 March 2015 diff hist −18 Purifying IgG1 from Hybridoma Supernatant No edit summary
- 10:3010:30, 27 March 2015 diff hist −8 Purifying IgG1 from Hybridoma Supernatant No edit summary
- 10:2910:29, 27 March 2015 diff hist +16 Whole mount ISH on axolotl tissue No edit summary current
- 10:2710:27, 27 March 2015 diff hist −12 ISH on axolotl tissue sections →Materials
- 10:2610:26, 27 March 2015 diff hist +31 ISH on axolotl tissue sections No edit summary
- 10:2310:23, 27 March 2015 diff hist +16 Double Immunostaining via antigen retrieval No edit summary
- 10:2210:22, 27 March 2015 diff hist +16 Alcian Blue Staining No edit summary current
- 10:0010:00, 27 March 2015 diff hist −23 Hybridoma cells in SFX media No edit summary
- 09:5909:59, 27 March 2015 diff hist −6 Hybridoma cells in SFX media No edit summary
- 09:5709:57, 27 March 2015 diff hist −14 Thawing (XB10) hybridoma cells No edit summary
- 09:5409:54, 27 March 2015 diff hist −14 Preparation of Low Serum for C2C12 cells No edit summary
- 09:1309:13, 27 March 2015 diff hist 0 C2C12 cell transfection with FuGene →Procedure
- 09:0609:06, 27 March 2015 diff hist +36 C2C12 cell transfection with FuGene No edit summary
- 09:0309:03, 27 March 2015 diff hist 0 C2C12 cell transfection with FuGene →Procedure