User contributions for Stephanh

23 March 2015

• 12:1812:18, 23 March 2015 diff hist +2‎ A1 cell transfection with FuGene ‎No edit summary
• 11:3611:36, 23 March 2015 diff hist −11‎ A1 cell transfection with FuGene ‎No edit summary
• 11:3211:32, 23 March 2015 diff hist −46‎ A1 cell transfection with FuGene ‎No edit summary
• 11:3111:31, 23 March 2015 diff hist +49‎ A1 cell transfection with FuGene ‎No edit summary
• 11:3011:30, 23 March 2015 diff hist −1‎ A1 cell transfection with FuGene ‎No edit summary
• 11:2911:29, 23 March 2015 diff hist −2‎ A1 cell transfection with FuGene ‎No edit summary
• 11:2811:28, 23 March 2015 diff hist −2‎ A1 cell transfection with FuGene ‎No edit summary
• 11:2711:27, 23 March 2015 diff hist −1‎ A1 cell transfection with FuGene ‎No edit summary
• 11:2711:27, 23 March 2015 diff hist +2‎ A1 cell transfection with FuGene ‎No edit summary
• 11:2111:21, 23 March 2015 diff hist +1,463‎ N A1 cell transfection with FuGene ‎ Created page with "== Materials == * Fugene Transfection solution * Complete media for A1 cells * No serum media * ATE * APBS * 10 cm dishes * eppendorf tubes == Procedure == Incubation FuGen..."

11 March 2015

• 08:4708:47, 11 March 2015 diff hist +14‎ Cardiomyocyte Preparation ‎No edit summary
• 08:4608:46, 11 March 2015 diff hist +1‎ Cardiomyocyte Preparation ‎No edit summary
• 08:4408:44, 11 March 2015 diff hist −15‎ Cardiomyocyte Preparation ‎No edit summary
• 08:4108:41, 11 March 2015 diff hist +4‎ Cardiomyocyte Preparation ‎No edit summary
• 08:4008:40, 11 March 2015 diff hist +12‎ Cardiomyocyte Preparation ‎No edit summary
• 08:3808:38, 11 March 2015 diff hist 0‎ Preparation of Low Serum for C2C12 cells ‎No edit summary
• 08:3608:36, 11 March 2015 diff hist 0‎ Thawing 293FT cells ‎No edit summary
• 08:3408:34, 11 March 2015 diff hist +2‎ Preparation of Low Serum for C2C12 cells ‎No edit summary
• 08:3208:32, 11 March 2015 diff hist 0‎ Preparation of Low Serum for C2C12 cells ‎No edit summary
• 08:3108:31, 11 March 2015 diff hist −8‎ Preparation of Low Serum for C2C12 cells ‎No edit summary
• 08:3008:30, 11 March 2015 diff hist +5‎ Preparation of Low Serum for C2C12 cells ‎No edit summary
• 08:3008:30, 11 March 2015 diff hist +475‎ N Preparation of Low Serum for C2C12 cells ‎ Created page with "== materials == <li> 390 mls DMEM-1 tube peni./ strepto. <li> tube glutamine (no insulin) <li> 8 mls Horse serum-1 sterile bottle <li> 500 ml sterile cylinder sterile fi..."

16 February 2015

• 08:2808:28, 16 February 2015 diff hist −51‎ Labelling myosin antibody ‎No edit summary
• 08:2008:20, 16 February 2015 diff hist −2‎ Labelling myosin antibody ‎No edit summary
• 08:1808:18, 16 February 2015 diff hist +20‎ Labelling myosin antibody ‎No edit summary
• 08:1408:14, 16 February 2015 diff hist +4‎ Labelling myosin antibody ‎No edit summary
• 08:1408:14, 16 February 2015 diff hist +36‎ Labelling myosin antibody ‎No edit summary
• 08:1008:10, 16 February 2015 diff hist +978‎ N Labelling myosin antibody ‎ Created page with "0.2 M Borate buffer, pH 8 Biorad protein assay substance IgG standard Anti myosin antibody (4A1025) FlNHS (1.5 mg/ml in DMSO—make up fresh just before use) 100mM glycine, pH..."

13 February 2015

• 10:2510:25, 13 February 2015 diff hist −2‎ Preparation of High Serum for A1 cells ‎No edit summary
• 10:2510:25, 13 February 2015 diff hist +3‎ Preparation of High Serum for A1 cells ‎No edit summary
• 10:2410:24, 13 February 2015 diff hist +5‎ Preparation of High Serum for A1 cells ‎No edit summary
• 10:2210:22, 13 February 2015 diff hist −1‎ Preparation of High Serum for A1 cells ‎No edit summary
• 10:2010:20, 13 February 2015 diff hist 0‎ Preparation of High Serum for A1 cells ‎No edit summary
• 10:1910:19, 13 February 2015 diff hist +7‎ Preparation of High Serum for A1 cells ‎No edit summary
• 10:1610:16, 13 February 2015 diff hist +5‎ Preparation of High Serum for A1 cells ‎No edit summary
• 10:1510:15, 13 February 2015 diff hist +16‎ Preparation of High Serum for A1 cells ‎No edit summary
• 10:0910:09, 13 February 2015 diff hist +4‎ Preparation of High Serum for A1 cells ‎No edit summary
• 10:0710:07, 13 February 2015 diff hist +465‎ N Preparation of High Serum for A1 cells ‎ Created page with "For 400 ml FCS 40 ml Eagle’s MEM 252 ml DdH2O 100 ml Pen/ Strep 4 ml Glutamin 4 ml Insulin (stock: 1mg/ ml) 4 ml - grow at 25ºC in 2% CO2 - if you want to gr..."
• 08:4008:40, 13 February 2015 diff hist −14‎ Alcian Blue Staining ‎No edit summary
• 08:3808:38, 13 February 2015 diff hist +599‎ N Alcian Blue Staining ‎ Created page with "# Fix sample O/N in 4% PFA freshly made # Wash in PBS, 3X5mins, 3X30mins 3. Wash 20mins in PBST 4. Equilibrate in EtOH, 25, 50%, 10 mins each 5. Place in Alcian blue staini..."

9 February 2015

• 08:4208:42, 9 February 2015 diff hist −2‎ Myoblasts electroporation ‎No edit summary
• 08:3608:36, 9 February 2015 diff hist +6‎ Myoblasts electroporation ‎No edit summary
• 08:3508:35, 9 February 2015 diff hist +4‎ Myoblasts electroporation ‎No edit summary
• 08:3008:30, 9 February 2015 diff hist −9‎ Letter to Froppier (French) ‎No edit summary current

2 February 2015

• 09:2909:29, 2 February 2015 diff hist +265‎ Letter to Froppier (French) ‎No edit summary

12 January 2015

• 13:0013:00, 12 January 2015 diff hist +16‎ Freezing of A1 cells ‎No edit summary
• 12:5412:54, 12 January 2015 diff hist +2‎ Electroporation of A1 cells ‎No edit summary
• 12:3812:38, 12 January 2015 diff hist +2‎ Cloning of C2C12 cells ‎No edit summary
• 12:3712:37, 12 January 2015 diff hist −7‎ Cloning of C2C12 cells ‎No edit summary
• 12:3212:32, 12 January 2015 diff hist +7‎ Cloning of C2C12 cells ‎No edit summary