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- 23:52, 18 July 2022 Frog Prrx1:CreER line screening (hist | edit) [3,512 bytes] Tzi.lin (talk | contribs) (Created page with "Transgenic TLM115 Frog Screening Protocol 2022.07.18 Tzi-Yang A. Mating (ask Birgit if not clear), ~1 hour 1. Choose the mating pairs and make notes on the frog list. • E....")
- 23:09, 18 July 2022 For cell transplantation (hist | edit) [1,171 bytes] Tzi.lin (talk | contribs) (Created page with "= Cell transplantation = ---- <li>(Continuing directly from Liberase dissociation or from FACS)</li> *Resuspend the cell...")
- 22:33, 18 July 2022 LiberaseTM cell dissociation protocol (hist | edit) [2,719 bytes] Tzi.lin (talk | contribs) (Created page with "= Prepare Reagents = ---- === 0.7x PBS === *Dilute 1x PBS to 0.7x with dH2O :-> Chill on ice before use. === Liberase TM solution === *40uL Stock Liberase TM (100x, 26WU/mL)...")
- 22:23, 18 July 2022 For scRNA-seq (hist | edit) [716 bytes] Tzi.lin (talk | contribs) (Created page with "= scRNA-seq = ---- <li>(Continue from the [[|dissociation protocol]])</li> *After 30um filter cup, immediately filter through 10um filter cup Resuspend the cell pellet with 15...")
- 22:14, 18 July 2022 For FACS (hist | edit) [537 bytes] Tzi.lin (talk | contribs) (Created page with "= FACS = ---- <li>(Continue from the dissociation protocol)</li> *Resuspend the cell pellet with 150-300uL AMEM(0) depending on the size of the pellet. *Immediately sort the c...")
- 21:40, 18 July 2022 LiberaseTM cell dissociation for axolotl/frog limb cells (hist | edit) [2,437 bytes] Tzi.lin (talk | contribs) (Created page with "= Prepare Reagents = ---- ‘’’0.7x PBS’’’ *Dilute 1x PBS to 0.7x with dH2O :-> Chill on ice before use")
- 21:07, 18 July 2022 With FACS (hist | edit) [623 bytes] Tzi.lin (talk | contribs) (Created page with "= For FACS = ---- *Finish the dissociation protocol *Resuspend in 150-200uL AMEM(0) *Send to FACS facility immediately **Book FACS machine F02 because it has both GFP and mChe...")
- 20:11, 18 July 2022 LiberaseTM dissociation (hist | edit) [2,467 bytes] Tzi.lin (talk | contribs) (Created page with "==== Preparing reagents ====")