Blastema cells: Difference between revisions
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(Created page with "== Composition for A1/BL cells == <ul> <li>250 ml MEM 21090</li> <li>100 ml H2O</li> <li>40 ml FCS for A1 cells</li> <li>4 ml pen/strep</li> <li>4 ml insulin</li> ...") |
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{{Tip|Filter the medium after mixing!}} | {{Tip|Filter the medium after mixing!}} | ||
== PBS == | == PBS == | ||
Revision as of 16:40, 29 November 2012
Composition for A1/BL cells
- 250 ml MEM 21090
- 100 ml H2O
- 40 ml FCS for A1 cells
- 4 ml pen/strep
- 4 ml insulin
- 4 ml glutamin
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NOTE: Filter the medium after mixing!
PBS
Dilute normal PBS (w/o bivalent cations) in water (both are supplied - cupboard in the corridor in opposite of the cell culture).
The final PBS concentration must be 80%.
Trypsin
Dilute 10xTE > in the -20°C freezer in the cell culture preparation room (black labelling) with APBS.