Preparation of Freezing media for C2C12 cells: Difference between revisions
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* 25 ml FCS (50%) | * 25 ml FCS (50%) | ||
Latest revision as of 14:34, 8 June 2015
Material
- 25 ml FCS (50%)
- 20 ml DMEM + Pen/ Strep + Glut (40%)
- 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro
Procedure
- put all stuffs together
- filtate the medium and aliquote the freezing media into 4 ml per tube
- store at –20ºC