Preparation of Freezing media for C2C12 cells: Difference between revisions
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== | == Procedure == | ||
# put all stuffs together | # put all stuffs together | ||
# filtate the medium and aliquote the freezing media into 4 ml per tube | # filtate the medium and aliquote the freezing media into 4 ml per tube | ||
# store at –20ºC | # store at –20ºC | ||
Revision as of 09:05, 25 March 2015
Materials
- 25 ml FCS (50%)
- 20 ml DMEM + Pen/ Strep + Glut (40%)
- 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro
Procedure
- put all stuffs together
- filtate the medium and aliquote the freezing media into 4 ml per tube
- store at –20ºC