Preparation of Freezing media for C2C12 cells: Difference between revisions
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(Created page with "Materials: 25 ml FCS (50%) 20 ml DMEM + Pen/ Strep + Glut (40%) 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro Procedure: - put all stuffs together - filtate the mediu...") |
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Materials | == Materials == | ||
25 ml FCS (50%) | * 25 ml FCS (50%) | ||
20 ml DMEM + Pen/ Strep + Glut (40%) | * 20 ml DMEM + Pen/ Strep + Glut (40%) | ||
5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro | * 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro | ||
== Execution == | |||
# put all stuffs together | |||
# filtate the medium and aliquote the freezing media into 4 ml per tube | |||
# store at –20ºC | |||
Revision as of 08:53, 25 March 2015
Materials
- 25 ml FCS (50%)
- 20 ml DMEM + Pen/ Strep + Glut (40%)
- 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro
Execution
- put all stuffs together
- filtate the medium and aliquote the freezing media into 4 ml per tube
- store at –20ºC