Preparation of Low Serum for C2C12 cells: Difference between revisions
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390 mls DMEM-1 tube peni./ strepto.
tube glutamine
(no insulin)
8 mls Horse serum-1 sterile bottle
500 ml sterile cylinder
sterile filter 150 ml
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== materials == | == materials == | ||
<li> 390 mls DMEM-1 tube peni./ strepto. | <li> 390 mls DMEM-1 tube peni./ strepto. | ||
<li> tube glutamine | <li> tube glutamine | ||
(no insulin) | (no insulin) | ||
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== execution == | == execution == | ||
# Put 390 mls DMEM into the cylinder | # Put 390 mls DMEM into the cylinder | ||
# Than add Peni./Strepto. ,Glutamine (no insulin) | # Than add Peni./Strepto. ,Glutamine (no insulin) | ||
Add 8 mls horse serum and than mix | |||
# filtrate the media through a sterile filter into the sterile bottle | # filtrate the media through a sterile filter into the sterile bottle | ||
# Label the bottle with “C2C12, differentiation media” , name and date | # Label the bottle with “C2C12, differentiation media” , name and date | ||
Revision as of 08:31, 11 March 2015
materials
execution
- Put 390 mls DMEM into the cylinder
- Than add Peni./Strepto. ,Glutamine (no insulin)
Add 8 mls horse serum and than mix
- filtrate the media through a sterile filter into the sterile bottle
- Label the bottle with “C2C12, differentiation media” , name and date