Preparation of Low Serum for C2C12 cells: Difference between revisions
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390 mls DMEM-1 tube peni./ strepto.
tube glutamine
(no insulin)
8 mls Horse serum-1 sterile bottle
500 ml sterile cylinder
sterile filter 150 ml
(Created page with "== materials == <li> 390 mls DMEM-1 tube peni./ strepto. <li> tube glutamine (no insulin) <li> 8 mls Horse serum-1 sterile bottle <li> 500 ml sterile cylinder sterile fi...") |
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== materials == | == materials == | ||
<li> 390 mls DMEM-1 tube peni./ strepto. | <li> 390 mls DMEM-1 tube peni./ strepto. </li> | ||
<li> tube glutamine | <li> tube glutamine | ||
(no insulin) | (no insulin) | ||
Revision as of 08:30, 11 March 2015
materials
execution
- Put 390 mls DMEM into the cylinder
- Than add Peni./Strepto. ,Glutamine (no insulin)
Add 8 mls horse serum and than mix
- filtrate the media through a sterile filter into the sterile bottle
- Label the bottle with “C2C12, differentiation media” , name and date