Preparation of Freezing media for C2C12 cells: Difference between revisions
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== Material == | |||
25 ml FCS (50%) | * 25 ml FCS (50%) | ||
20 ml DMEM + Pen/ Strep + Glut (40%) | * 20 ml DMEM + Pen/ Strep + Glut (40%) | ||
5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro | * 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro | ||
Procedure | == Procedure == | ||
# put all stuffs together | |||
# filtate the medium and aliquote the freezing media into 4 ml per tube | |||
# store at –20ºC | |||
Latest revision as of 14:34, 8 June 2015
Material
- 25 ml FCS (50%)
- 20 ml DMEM + Pen/ Strep + Glut (40%)
- 5 ml DMSO (10%) Catalog Number: D2650 37,80 Euro
Procedure
- put all stuffs together
- filtate the medium and aliquote the freezing media into 4 ml per tube
- store at –20ºC