Preparation of Low Serum for C2C12 cells: Difference between revisions
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== | == Material == | ||
* 390 mls DMEM-1 tube peni./ strepto. | |||
* tube glutamine | |||
(no insulin) | (no insulin) | ||
* 8 mls Horse serum-1 sterile bottle | |||
* 500 ml sterile cylinder | |||
sterile filter 150 ml | sterile filter 150 ml | ||
== Procedure | == Procedure == | ||
# Put 390 mls DMEM into the cylinder | # Put 390 mls DMEM into the cylinder | ||
# Than add Peni./Strepto. ,Glutamine (no insulin) Add 8 mls horse serum and than mix | # Than add Peni./Strepto. ,Glutamine (no insulin) Add 8 mls horse serum and than mix | ||
# filtrate the media through a sterile filter into the sterile bottle | # filtrate the media through a sterile filter into the sterile bottle | ||
# Label the bottle with “C2C12, differentiation media” , name and date | # Label the bottle with “C2C12, differentiation media” , name and date | ||
Latest revision as of 14:33, 8 June 2015
Material
- 390 mls DMEM-1 tube peni./ strepto.
- tube glutamine
(no insulin)
- 8 mls Horse serum-1 sterile bottle
- 500 ml sterile cylinder
sterile filter 150 ml
Procedure
- Put 390 mls DMEM into the cylinder
- Than add Peni./Strepto. ,Glutamine (no insulin) Add 8 mls horse serum and than mix
- filtrate the media through a sterile filter into the sterile bottle
- Label the bottle with “C2C12, differentiation media” , name and date