Difference between revisions of "With FACS"
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(Created page with "= For FACS = ---- *Finish the dissociation protocol *Resuspend in 150-200uL AMEM(0) *Send to FACS facility immediately **Book FACS machine F02 because it has both GFP and mChe...") |
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*Resuspend in 50-100uL AMEM(0) | *Resuspend in 50-100uL AMEM(0) | ||
*Count the cells | *Count the cells | ||
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| + | **The sample is ready for downstream applications: | ||
| + | ***[[For_scRNA-seq|scRNA-seq]] | ||
| + | ***[[For_transplantation|cell transplantation]] | ||
Revision as of 21:10, 18 July 2022
For FACS
- Finish the dissociation protocol
- Resuspend in 150-200uL AMEM(0)
- Send to FACS facility immediately
- Book FACS machine F02 because it has both GFP and mCherry lasers.
- Normal setting: 85um nozzle with 0.7x FACS Flow for frog cells; 100um nozzle with 0.7x FACS Flow for axolotl cells.
- Collect the cells into a 1.5mL tube with 700uL AMEM(0) inside. Note down the FACS count.
- Spin down the cells. 300xrcf, 20 degree, 5 minutes.
- Resuspend in 50-100uL AMEM(0)
- Count the cells
- The sample is ready for downstream applications: