Thawing frozen C2C12 cells

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Revision as of 08:34, 27 March 2015 by Stephanh (talk | contribs)
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Materials

  • frozen C2C12 cells
  • HS for C2C12 cells
  • pipettes and tips
  • FALCON tube 15 ml
  • Cell culture flask

Procedure

  • heat the HS up to 37ºC
  • pipette 14 ml C2C12 HS media in a 75 cm2 cell culture flask
  • pipette carefully 1 ml from the warm media in the cryo tube to the frozen cells
  • pipette carefully up and down till the cells are thaw
  • pipette all in the FALCON tube
  • centrifuge at 1000 xg, 3 min at 4ºC
  • label the cell culture flask with the name, date and C2C12 with the passage number
  • aspirate of the media and resuspend the cells in 1 ml of the new HS
  • pipette the suspension in the cell culture flask
  • check the cells under the microscope
  • put the flask at 37ºC, 10% CO2