Thawing (XB10) hybridoma cells

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Materials:

  • 1 x 15 ml conical tube per vial of frozen cells
  • water bath (37 C for mammalian cells)
  • High serum media (RPMI media +10% FCS+glutamine+P/S) [Use Defined Supplemented Calf Serum (DSCS) from Hyclone (Prebio) for making high serum media for hybridoma cells]
  • 1 blue cap flask
  • 1 x 10 ml pipettes
  • gestopfft pasteur pipettes
  • ungestopfft pasteur pipettes
  • frozen cells in liquid nitrogen or dry ice

    Execution:

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