Difference between revisions of "Thawing (XB10) hybridoma cells"
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1 x 15 ml conical tube per vial of frozen cells
water bath (37 C for mammalian cells)
High serum media (RPMI media +10% FCS+glutamine+P/S) [Use Defined Supplemented Calf Serum (DSCS) from Hyclone (Prebio) for making high serum media for hybridoma cells]
1 blue cap flask
1 x 10 ml pipettes
gestopfft pasteur pipettes
ungestopfft pasteur pipettes
frozen cells in liquid nitrogen or dry ice
| Line 2: | Line 2: | ||
<li>1 x 15 ml conical tube per vial of frozen cells | <li>1 x 15 ml conical tube per vial of frozen cells | ||
<li>water bath (37 C for mammalian cells) | <li>water bath (37 C for mammalian cells) | ||
| − | <li>High serum media (RPMI media +10% FCS+glutamine+P/S) [ | + | <li>High serum media (RPMI media +10% FCS+glutamine+P/S) [''Use Defined Supplemented Calf Serum (DSCS) from Hyclone (Prebio) for making high serum media for hybridoma cells''] |
<li>1 blue cap flask | <li>1 blue cap flask | ||
<li>1 x 10 ml pipettes | <li>1 x 10 ml pipettes | ||
<li>gestopfft pasteur pipettes | <li>gestopfft pasteur pipettes | ||
<li>ungestopfft pasteur pipettes | <li>ungestopfft pasteur pipettes | ||
| − | <li>frozen cells in liquid nitrogen or dry ice | + | <li>frozen cells in liquid nitrogen or dry ice |
| + | |||
| + | ==Execution:== | ||