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- 13:52, 15 June 2015 diff hist -12 Hybridoma cells clones →ELISA (step 1 & 4)
- 14:37, 8 June 2015 diff hist -2 Hybridoma cells clones
- 14:35, 8 June 2015 diff hist -1 Hybridoma cells in SFX media current
- 14:34, 8 June 2015 diff hist -1 Preparation of Freezing media for C2C12 cells current
- 14:34, 8 June 2015 diff hist -1 Preparation of High Serum for C2C12 cells current
- 14:33, 8 June 2015 diff hist -1 Preparation of Low Serum for C2C12 cells current
- 14:33, 8 June 2015 diff hist 0 Electroporation of C2C12 cells current
- 14:32, 8 June 2015 diff hist -3 Cloning of C2C12 cells current
- 14:32, 8 June 2015 diff hist -1 C2C12 cell transfection with FuGene current
- 14:31, 8 June 2015 diff hist -1 Myotube preparation of C2C12 cells current
- 14:31, 8 June 2015 diff hist -1 Passaging C2C12 cells current
- 14:30, 8 June 2015 diff hist -1 Freezing of C2C12 cells current
- 14:30, 8 June 2015 diff hist -1 Thawing frozen C2C12 cells current
- 14:29, 8 June 2015 diff hist -1 Thawing (XB10) hybridoma cells current
- 14:29, 8 June 2015 diff hist -1 MESC culture current
- 14:28, 8 June 2015 diff hist -1 Preparation of Freezing media for A1 cells current
- 14:28, 8 June 2015 diff hist -1 Preparation of High Serum for A1 cells →Materials current
- 14:26, 8 June 2015 diff hist -1 BrdU and myosin staining current
- 14:26, 8 June 2015 diff hist -1 Preparation of Low Serum for A1 cells current
- 14:25, 8 June 2015 diff hist -1 BrdU labelling of A1 cells in 96-well plate current
- 14:25, 8 June 2015 diff hist -1 Electroporation of A1 cells current
- 14:24, 8 June 2015 diff hist -1 A1 cell transfection with FuGene current
- 14:24, 8 June 2015 diff hist -1 Modified myotube prep current
- 14:23, 8 June 2015 diff hist -1 A1 cell passaging myotube current
- 14:23, 8 June 2015 diff hist 0 Passaging A1 cell current
- 14:21, 8 June 2015 diff hist -1 Passaging A1 cell
- 14:20, 8 June 2015 diff hist -1 Freezing of A1 cells current
- 14:20, 8 June 2015 diff hist -1 Thawing frozen A1 cells current
- 14:19, 8 June 2015 diff hist -1 G418 medium preparation current
- 14:18, 8 June 2015 diff hist -1 Thawing 293FT cells current
- 14:18, 8 June 2015 diff hist -1 Myoblasts electroporation current
- 14:17, 8 June 2015 diff hist -1 Adhesive cell culture current
- 14:17, 8 June 2015 diff hist +1 Adhesive cell culture
- 14:15, 8 June 2015 diff hist -2 Adhesive cell culture
- 14:12, 8 June 2015 diff hist -2 Adhesive cell culture
- 14:10, 8 June 2015 diff hist +28 Adhesive cell culture
- 14:06, 8 June 2015 diff hist +21 Adhesive cell culture
- 14:03, 8 June 2015 diff hist +17 Adhesive cell culture
- 13:55, 8 June 2015 diff hist +3,049 N Adhesive cell culture Created page with "ECM coating and composition of culture media These steps for preparation were carried out under sterile terms in cell culture room at clean bench. Gelatine: Gelatine (Sigma,..."
- 08:49, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue current
- 08:48, 1 June 2015 diff hist +6 TSA Staining on axolotl-tissue
- 08:40, 1 June 2015 diff hist -12 TSA Staining on axolotl-tissue
- 08:39, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue
- 08:37, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue
- 08:35, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue
- 08:34, 1 June 2015 diff hist +12 TSA Staining on axolotl-tissue
- 08:28, 1 June 2015 diff hist -20 TSA Staining on axolotl-tissue
- 08:24, 1 June 2015 diff hist -2 TSA Staining on axolotl-tissue
- 08:23, 1 June 2015 diff hist +1 TSA Staining on axolotl-tissue
- 08:22, 1 June 2015 diff hist -6 TSA Staining on axolotl-tissue
- 08:19, 1 June 2015 diff hist -10 TSA Staining on axolotl-tissue
- 08:18, 1 June 2015 diff hist +10 TSA Staining on axolotl-tissue
- 08:17, 1 June 2015 diff hist -14 TSA Staining on axolotl-tissue
- 08:14, 1 June 2015 diff hist -6 TSA Staining on axolotl-tissue
- 08:12, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue
- 08:06, 1 June 2015 diff hist +9 TSA Staining on axolotl-tissue
- 07:50, 1 June 2015 diff hist +1,096 N TSA Staining on axolotl-tissue Created page with "Solutions: - TNT wash buffer: 0.1M TRIS-HCL, pH 7.5 0.15M NaCl 0.05% Tween20 - TNB buffer: a) for blocking: 0.1M TRIS-HCL, pH 7.5 0.15M NaCl 20% goat serum b) for di..."
- 14:11, 4 May 2015 diff hist -16 MESC culture
- 14:06, 4 May 2015 diff hist 0 MESC culture
- 10:07, 4 May 2015 diff hist -11 MESC culture
- 10:06, 4 May 2015 diff hist -4 MESC culture
- 10:04, 4 May 2015 diff hist +19 MESC culture
- 10:02, 4 May 2015 diff hist +17 MESC culture
- 08:43, 4 May 2015 diff hist +4,160 N MESC culture Created page with "46C-ES cells/cyst formation Thawing of 46C ES cells: Prepare the growth medium: 405 ml DMEM + high glucose * Glutamax (Invitrogen: 31966) 75 ml FCS 5 ml L-Gln 100x (Invitro..."
- 13:20, 17 April 2015 diff hist 0 Electroporation of C2C12 cells
- 13:20, 17 April 2015 diff hist -1 Electroporation of C2C12 cells →material
- 13:17, 17 April 2015 diff hist 0 Electroporation of C2C12 cells →material
- 13:15, 17 April 2015 diff hist +1,228 N Electroporation of C2C12 cells Created page with "== material == Transfection * 1x Steinberger Medium * 1x DMEM without serum - remove the old media - wash the cells with 5 ml PBS - remove the PBS - add 2 ml TE - put the..."
- 12:58, 17 April 2015 diff hist 0 Preparation of Freezing media for A1 cells →Procedure
- 12:54, 17 April 2015 diff hist +11 Preparation of Freezing media for A1 cells
- 12:52, 17 April 2015 diff hist +432 N Preparation of Freezing media for A1 cells Created page with "Materials * DMSO (one ampules from Sigma) * High serum for A1 cells * 2x 50 ml FALCON tubes * 1x 15 ml FALCON tube * Pipettes ** 10x 5 ml FALCON tubes Procedure - clean the a..."
- 12:45, 17 April 2015 diff hist 0 Preparation of Low Serum for A1 cells →Procedure
- 12:44, 17 April 2015 diff hist 0 Preparation of Low Serum for A1 cells →Materials
- 12:40, 17 April 2015 diff hist +261 N Preparation of Low Serum for A1 cells Created page with "== Materials == - 280 ml MEM - 100 ml sterile water - 1 tube Pen/ Strep (4ml) - 1 tube Glutamine (4ml) - 1 tube Insulin (4 ml) - 2 ml FCS - 500 ml filter with bottle == Proce..."
- 10:54, 27 March 2015 diff hist -22 RNA formaldehyde gels current
- 10:52, 27 March 2015 diff hist -3 RNA formaldehyde gels
- 10:51, 27 March 2015 diff hist +13 RNA formaldehyde gels
- 10:48, 27 March 2015 diff hist -17 RNA Extraction from axolotl tissue
- 10:45, 27 March 2015 diff hist -22 Digestion DNA →Digestion current
- 10:44, 27 March 2015 diff hist -28 Digestion DNA
- 10:42, 27 March 2015 diff hist +8 Digestion DNA
- 10:40, 27 March 2015 diff hist +16 Metamorphosis protocol current
- 10:39, 27 March 2015 diff hist +16 Antibody staining current
- 10:36, 27 March 2015 diff hist +11 Labeled AB staining
- 10:33, 27 March 2015 diff hist +4 Labelling Pax6 with Dig-NHS current
- 10:32, 27 March 2015 diff hist -5 Labelling myosin antibody
- 10:31, 27 March 2015 diff hist -18 Purifying IgG1 from Hybridoma Supernatant
- 10:30, 27 March 2015 diff hist -8 Purifying IgG1 from Hybridoma Supernatant
- 10:29, 27 March 2015 diff hist +16 Whole mount ISH on axolotl tissue current
- 10:27, 27 March 2015 diff hist -12 ISH on axolotl tissue sections →Materials
- 10:26, 27 March 2015 diff hist +31 ISH on axolotl tissue sections
- 10:23, 27 March 2015 diff hist +16 Double Immunostaining via antigen retrieval
- 10:22, 27 March 2015 diff hist +16 Alcian Blue Staining current
- 10:00, 27 March 2015 diff hist -23 Hybridoma cells in SFX media
- 09:59, 27 March 2015 diff hist -6 Hybridoma cells in SFX media
- 09:57, 27 March 2015 diff hist -14 Thawing (XB10) hybridoma cells
- 09:54, 27 March 2015 diff hist -14 Preparation of Low Serum for C2C12 cells
- 09:13, 27 March 2015 diff hist 0 C2C12 cell transfection with FuGene →Procedure
- 09:06, 27 March 2015 diff hist +36 C2C12 cell transfection with FuGene
- 09:03, 27 March 2015 diff hist 0 C2C12 cell transfection with FuGene →Procedure