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- 08:19, 1 June 2015 diff hist -10 TSA Staining on axolotl-tissue
- 08:18, 1 June 2015 diff hist +10 TSA Staining on axolotl-tissue
- 08:17, 1 June 2015 diff hist -14 TSA Staining on axolotl-tissue
- 08:14, 1 June 2015 diff hist -6 TSA Staining on axolotl-tissue
- 08:12, 1 June 2015 diff hist 0 TSA Staining on axolotl-tissue
- 08:06, 1 June 2015 diff hist +9 TSA Staining on axolotl-tissue
- 07:50, 1 June 2015 diff hist +1,096 N TSA Staining on axolotl-tissue Created page with "Solutions: - TNT wash buffer: 0.1M TRIS-HCL, pH 7.5 0.15M NaCl 0.05% Tween20 - TNB buffer: a) for blocking: 0.1M TRIS-HCL, pH 7.5 0.15M NaCl 20% goat serum b) for di..."
- 14:11, 4 May 2015 diff hist -16 MESC culture
- 14:06, 4 May 2015 diff hist 0 MESC culture
- 10:07, 4 May 2015 diff hist -11 MESC culture
- 10:06, 4 May 2015 diff hist -4 MESC culture
- 10:04, 4 May 2015 diff hist +19 MESC culture
- 10:02, 4 May 2015 diff hist +17 MESC culture
- 08:43, 4 May 2015 diff hist +4,160 N MESC culture Created page with "46C-ES cells/cyst formation Thawing of 46C ES cells: Prepare the growth medium: 405 ml DMEM + high glucose * Glutamax (Invitrogen: 31966) 75 ml FCS 5 ml L-Gln 100x (Invitro..."
- 13:20, 17 April 2015 diff hist 0 Electroporation of C2C12 cells
- 13:20, 17 April 2015 diff hist -1 Electroporation of C2C12 cells →material
- 13:17, 17 April 2015 diff hist 0 Electroporation of C2C12 cells →material
- 13:15, 17 April 2015 diff hist +1,228 N Electroporation of C2C12 cells Created page with "== material == Transfection * 1x Steinberger Medium * 1x DMEM without serum - remove the old media - wash the cells with 5 ml PBS - remove the PBS - add 2 ml TE - put the..."
- 12:58, 17 April 2015 diff hist 0 Preparation of Freezing media for A1 cells →Procedure
- 12:54, 17 April 2015 diff hist +11 Preparation of Freezing media for A1 cells
- 12:52, 17 April 2015 diff hist +432 N Preparation of Freezing media for A1 cells Created page with "Materials * DMSO (one ampules from Sigma) * High serum for A1 cells * 2x 50 ml FALCON tubes * 1x 15 ml FALCON tube * Pipettes ** 10x 5 ml FALCON tubes Procedure - clean the a..."
- 12:45, 17 April 2015 diff hist 0 Preparation of Low Serum for A1 cells →Procedure
- 12:44, 17 April 2015 diff hist 0 Preparation of Low Serum for A1 cells →Materials
- 12:40, 17 April 2015 diff hist +261 N Preparation of Low Serum for A1 cells Created page with "== Materials == - 280 ml MEM - 100 ml sterile water - 1 tube Pen/ Strep (4ml) - 1 tube Glutamine (4ml) - 1 tube Insulin (4 ml) - 2 ml FCS - 500 ml filter with bottle == Proce..."
- 10:54, 27 March 2015 diff hist -22 RNA formaldehyde gels current
- 10:52, 27 March 2015 diff hist -3 RNA formaldehyde gels
- 10:51, 27 March 2015 diff hist +13 RNA formaldehyde gels
- 10:48, 27 March 2015 diff hist -17 RNA Extraction from axolotl tissue
- 10:45, 27 March 2015 diff hist -22 Digestion DNA →Digestion current
- 10:44, 27 March 2015 diff hist -28 Digestion DNA
- 10:42, 27 March 2015 diff hist +8 Digestion DNA
- 10:40, 27 March 2015 diff hist +16 Metamorphosis protocol current
- 10:39, 27 March 2015 diff hist +16 Antibody staining current
- 10:36, 27 March 2015 diff hist +11 Labeled AB staining
- 10:33, 27 March 2015 diff hist +4 Labelling Pax6 with Dig-NHS current
- 10:32, 27 March 2015 diff hist -5 Labelling myosin antibody
- 10:31, 27 March 2015 diff hist -18 Purifying IgG1 from Hybridoma Supernatant
- 10:30, 27 March 2015 diff hist -8 Purifying IgG1 from Hybridoma Supernatant
- 10:29, 27 March 2015 diff hist +16 Whole mount ISH on axolotl tissue current
- 10:27, 27 March 2015 diff hist -12 ISH on axolotl tissue sections →Materials
- 10:26, 27 March 2015 diff hist +31 ISH on axolotl tissue sections
- 10:23, 27 March 2015 diff hist +16 Double Immunostaining via antigen retrieval
- 10:22, 27 March 2015 diff hist +16 Alcian Blue Staining current
- 10:00, 27 March 2015 diff hist -23 Hybridoma cells in SFX media
- 09:59, 27 March 2015 diff hist -6 Hybridoma cells in SFX media
- 09:57, 27 March 2015 diff hist -14 Thawing (XB10) hybridoma cells
- 09:54, 27 March 2015 diff hist -14 Preparation of Low Serum for C2C12 cells
- 09:13, 27 March 2015 diff hist 0 C2C12 cell transfection with FuGene →Procedure
- 09:06, 27 March 2015 diff hist +36 C2C12 cell transfection with FuGene
- 09:03, 27 March 2015 diff hist 0 C2C12 cell transfection with FuGene →Procedure