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- 12:56, 3 November 2014 diff hist +28 C2C12 cell transfection with FuGene
- 12:52, 3 November 2014 diff hist +2 C2C12 cell transfection with FuGene
- 12:51, 3 November 2014 diff hist 0 C2C12 cell transfection with FuGene
- 12:47, 3 November 2014 diff hist 0 C2C12 cell transfection with FuGene
- 12:43, 3 November 2014 diff hist +1 C2C12 cell transfection with FuGene
- 12:41, 3 November 2014 diff hist -4 C2C12 cell transfection with FuGene
- 12:38, 3 November 2014 diff hist +2 C2C12 cell transfection with FuGene
- 12:36, 3 November 2014 diff hist -42 C2C12 cell transfection with FuGene
- 12:34, 3 November 2014 diff hist +9 C2C12 cell transfection with FuGene
- 12:33, 3 November 2014 diff hist -1 C2C12 cell transfection with FuGene
- 12:31, 3 November 2014 diff hist 0 C2C12 cell transfection with FuGene
- 12:29, 3 November 2014 diff hist +12 C2C12 cell transfection with FuGene
- 12:28, 3 November 2014 diff hist -59 C2C12 cell transfection with FuGene
- 12:19, 3 November 2014 diff hist +3 C2C12 cell transfection with FuGene
- 12:19, 3 November 2014 diff hist +127 C2C12 cell transfection with FuGene
- 12:17, 3 November 2014 diff hist +1 C2C12 cell transfection with FuGene
- 12:16, 3 November 2014 diff hist -1 C2C12 cell transfection with FuGene
- 12:15, 3 November 2014 diff hist -2 C2C12 cell transfection with FuGene
- 12:15, 3 November 2014 diff hist +3 C2C12 cell transfection with FuGene
- 12:14, 3 November 2014 diff hist -1 C2C12 cell transfection with FuGene
- 10:55, 3 November 2014 diff hist +1 C2C12 cell transfection with FuGene
- 10:55, 3 November 2014 diff hist -21 C2C12 cell transfection with FuGene
- 10:52, 3 November 2014 diff hist +7 C2C12 cell transfection with FuGene
- 10:48, 3 November 2014 diff hist +2 C2C12 cell transfection with FuGene
- 10:46, 3 November 2014 diff hist +14 C2C12 cell transfection with FuGene
- 10:35, 3 November 2014 diff hist +131 C2C12 cell transfection with FuGene
- 10:30, 3 November 2014 diff hist +12 C2C12 cell transfection with FuGene
- 10:28, 3 November 2014 diff hist +4 C2C12 cell transfection with FuGene
- 10:25, 3 November 2014 diff hist +3,695 N C2C12 cell transfection with FuGene Created page with "2 x 6cm dishes with C2C12 cells Materials: Day1: 2x 6cm dishes 75 cm2 flask C2C12 cells in a 75 cm2 flask 15 ml FALCON tubes High serum media for C2C12 TE in PBS PBS ..."
- 07:41, 24 October 2014 diff hist 0 Freezing of C2C12 cells
- 07:35, 24 October 2014 diff hist -1 Freezing of C2C12 cells
- 07:35, 24 October 2014 diff hist +8 Freezing of C2C12 cells
- 07:33, 24 October 2014 diff hist -1 Freezing of C2C12 cells
- 07:25, 24 October 2014 diff hist +707 N Freezing of C2C12 cells Created page with "Materials: - 15 ml FALCON tube - PBS w/o Ca2+ and Mg2+ - TE in PBS - Freezing media (10% DMSO + 40% serum + 50% DMEM) - Pipettes and tips - Glas pipetts - cryo tube - ice - f..."
- 10:13, 22 October 2014 diff hist +3 Hybridoma cells clones
- 10:12, 22 October 2014 diff hist -3 Hybridoma cells clones
- 10:10, 22 October 2014 diff hist +2 Hybridoma cells clones →Procedure:
- 09:59, 22 October 2014 diff hist +2 Hybridoma cells clones
- 09:56, 22 October 2014 diff hist -40 Hybridoma cells clones →Procedure:
- 09:55, 22 October 2014 diff hist +38 Hybridoma cells clones →Procedure:
- 09:54, 22 October 2014 diff hist -20 Hybridoma cells clones
- 09:49, 22 October 2014 diff hist -6 Hybridoma cells clones
- 09:45, 22 October 2014 diff hist -7 Hybridoma cells clones
- 09:41, 22 October 2014 diff hist +8 Hybridoma cells clones
- 09:36, 22 October 2014 diff hist +12 Hybridoma cells clones
- 09:35, 22 October 2014 diff hist +5 Hybridoma cells clones
- 09:34, 22 October 2014 diff hist -2 Hybridoma cells clones
- 09:31, 22 October 2014 diff hist -7 Hybridoma cells clones
- 09:30, 22 October 2014 diff hist -24 Hybridoma cells clones
- 09:23, 22 October 2014 diff hist +11,002 N Hybridoma cells clones Created page with "== General execution: == 1) ELISA of the supernatants 2) Antibody staining on cryostat section (7 dpa tail sections) of the positive clones from the ELISA 3) Cloning of a p..."
- 07:44, 20 October 2014 diff hist +115 ISH on axolotl tissue sections
- 07:40, 20 October 2014 diff hist -6 ISH on axolotl tissue sections
- 07:39, 20 October 2014 diff hist -2 ISH on axolotl tissue sections
- 07:38, 20 October 2014 diff hist -4 ISH on axolotl tissue sections
- 07:34, 20 October 2014 diff hist +1 ISH on axolotl tissue sections
- 07:29, 20 October 2014 diff hist +1 ISH on axolotl tissue sections
- 07:26, 20 October 2014 diff hist +12 ISH on axolotl tissue sections
- 11:57, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:57, 13 October 2014 diff hist +15 ISH on axolotl tissue sections
- 11:55, 13 October 2014 diff hist +8 ISH on axolotl tissue sections
- 11:48, 13 October 2014 diff hist +3 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist -7 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist +13 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:46, 13 October 2014 diff hist +18 ISH on axolotl tissue sections
- 11:45, 13 October 2014 diff hist 0 ISH on axolotl tissue sections
- 11:44, 13 October 2014 diff hist -8 ISH on axolotl tissue sections
- 11:42, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:41, 13 October 2014 diff hist -1 ISH on axolotl tissue sections
- 11:40, 13 October 2014 diff hist -5 ISH on axolotl tissue sections
- 11:39, 13 October 2014 diff hist -5 ISH on axolotl tissue sections
- 11:35, 13 October 2014 diff hist +5,086 N ISH on axolotl tissue sections Created page with "== Probe preparation == 1. Mix the following at RT: 10x transcription buffer 2 µl dig Nucleotide mix 2 µl Linearized plasmid x µl (1µg) RNase inhibito..."
- 08:02, 10 October 2014 diff hist +1 Modified myotube prep
- 08:01, 10 October 2014 diff hist +7 Modified myotube prep
- 08:00, 10 October 2014 diff hist +4 Modified myotube prep
- 07:59, 10 October 2014 diff hist +1 Cardiomyocyte Preparation
- 07:58, 10 October 2014 diff hist -3 Cardiomyocyte Preparation
- 07:56, 10 October 2014 diff hist -1 Cardiomyocyte Preparation
- 07:54, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:54, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:53, 10 October 2014 diff hist -2 Cardiomyocyte Preparation
- 07:52, 10 October 2014 diff hist +4 Cardiomyocyte Preparation
- 07:51, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:50, 10 October 2014 diff hist +6 Cardiomyocyte Preparation
- 07:46, 10 October 2014 diff hist -10 Modified myotube prep
- 07:43, 10 October 2014 diff hist -56 Modified myotube prep
- 07:37, 10 October 2014 diff hist 0 Modified myotube prep →Execution
- 07:36, 10 October 2014 diff hist +5 Modified myotube prep
- 07:34, 10 October 2014 diff hist -38 Modified myotube prep
- 07:29, 10 October 2014 diff hist -1 Modified myotube prep
- 07:10, 10 October 2014 diff hist +7 Modified myotube prep
- 07:05, 10 October 2014 diff hist +3,246 N Modified myotube prep Created page with "Induce A1 cells on 10 cm plates to form myotubes by * wash 1x with 5 mls APBS * changing the media to 0.5% serum-AMEM * wait 4 days If desired, myotubes can be purified awa..."
- 08:09, 26 September 2014 diff hist +4 Passaging A1 cell
- 08:07, 26 September 2014 diff hist +6 Passaging A1 cell
- 08:06, 26 September 2014 diff hist +787 Passaging A1 cell
- 07:44, 24 September 2014 diff hist +4 Thawing frozen A1 cells
- 07:43, 24 September 2014 diff hist 0 Thawing frozen A1 cells
- 07:37, 24 September 2014 diff hist +828 N Thawing frozen A1 cells Created page with "== Materials: == - Gelatin (warmed to 37ºC) - HS for A1 cells - 1x 15 ml FALCON tubes - Pipettes - 1x 175 cm2 flasks - frozen cells into liquid nitrogen == Procedure: == - p..."
- 08:45, 5 September 2014 diff hist +2 Electroporation of A1 cells
- 08:45, 5 September 2014 diff hist +15 Electroporation of A1 cells