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- 09:41, 22 October 2014 diff hist +8 Hybridoma cells clones
- 09:36, 22 October 2014 diff hist +12 Hybridoma cells clones
- 09:35, 22 October 2014 diff hist +5 Hybridoma cells clones
- 09:34, 22 October 2014 diff hist -2 Hybridoma cells clones
- 09:31, 22 October 2014 diff hist -7 Hybridoma cells clones
- 09:30, 22 October 2014 diff hist -24 Hybridoma cells clones
- 09:23, 22 October 2014 diff hist +11,002 N Hybridoma cells clones Created page with "== General execution: == 1) ELISA of the supernatants 2) Antibody staining on cryostat section (7 dpa tail sections) of the positive clones from the ELISA 3) Cloning of a p..."
- 07:44, 20 October 2014 diff hist +115 ISH on axolotl tissue sections
- 07:40, 20 October 2014 diff hist -6 ISH on axolotl tissue sections
- 07:39, 20 October 2014 diff hist -2 ISH on axolotl tissue sections
- 07:38, 20 October 2014 diff hist -4 ISH on axolotl tissue sections
- 07:34, 20 October 2014 diff hist +1 ISH on axolotl tissue sections
- 07:29, 20 October 2014 diff hist +1 ISH on axolotl tissue sections
- 07:26, 20 October 2014 diff hist +12 ISH on axolotl tissue sections
- 11:57, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:57, 13 October 2014 diff hist +15 ISH on axolotl tissue sections
- 11:55, 13 October 2014 diff hist +8 ISH on axolotl tissue sections
- 11:48, 13 October 2014 diff hist +3 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist -7 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist +13 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:46, 13 October 2014 diff hist +18 ISH on axolotl tissue sections
- 11:45, 13 October 2014 diff hist 0 ISH on axolotl tissue sections
- 11:44, 13 October 2014 diff hist -8 ISH on axolotl tissue sections
- 11:42, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:41, 13 October 2014 diff hist -1 ISH on axolotl tissue sections
- 11:40, 13 October 2014 diff hist -5 ISH on axolotl tissue sections
- 11:39, 13 October 2014 diff hist -5 ISH on axolotl tissue sections
- 11:35, 13 October 2014 diff hist +5,086 N ISH on axolotl tissue sections Created page with "== Probe preparation == 1. Mix the following at RT: 10x transcription buffer 2 µl dig Nucleotide mix 2 µl Linearized plasmid x µl (1µg) RNase inhibito..."
- 08:02, 10 October 2014 diff hist +1 Modified myotube prep
- 08:01, 10 October 2014 diff hist +7 Modified myotube prep
- 08:00, 10 October 2014 diff hist +4 Modified myotube prep
- 07:59, 10 October 2014 diff hist +1 Cardiomyocyte Preparation
- 07:58, 10 October 2014 diff hist -3 Cardiomyocyte Preparation
- 07:56, 10 October 2014 diff hist -1 Cardiomyocyte Preparation
- 07:54, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:54, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:53, 10 October 2014 diff hist -2 Cardiomyocyte Preparation
- 07:52, 10 October 2014 diff hist +4 Cardiomyocyte Preparation
- 07:51, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:50, 10 October 2014 diff hist +6 Cardiomyocyte Preparation
- 07:46, 10 October 2014 diff hist -10 Modified myotube prep
- 07:43, 10 October 2014 diff hist -56 Modified myotube prep
- 07:37, 10 October 2014 diff hist 0 Modified myotube prep →Execution
- 07:36, 10 October 2014 diff hist +5 Modified myotube prep
- 07:34, 10 October 2014 diff hist -38 Modified myotube prep
- 07:29, 10 October 2014 diff hist -1 Modified myotube prep
- 07:10, 10 October 2014 diff hist +7 Modified myotube prep
- 07:05, 10 October 2014 diff hist +3,246 N Modified myotube prep Created page with "Induce A1 cells on 10 cm plates to form myotubes by * wash 1x with 5 mls APBS * changing the media to 0.5% serum-AMEM * wait 4 days If desired, myotubes can be purified awa..."
- 08:09, 26 September 2014 diff hist +4 Passaging A1 cell
- 08:07, 26 September 2014 diff hist +6 Passaging A1 cell
- 08:06, 26 September 2014 diff hist +787 Passaging A1 cell
- 07:44, 24 September 2014 diff hist +4 Thawing frozen A1 cells
- 07:43, 24 September 2014 diff hist 0 Thawing frozen A1 cells
- 07:37, 24 September 2014 diff hist +828 N Thawing frozen A1 cells Created page with "== Materials: == - Gelatin (warmed to 37ºC) - HS for A1 cells - 1x 15 ml FALCON tubes - Pipettes - 1x 175 cm2 flasks - frozen cells into liquid nitrogen == Procedure: == - p..."
- 08:45, 5 September 2014 diff hist +2 Electroporation of A1 cells
- 08:45, 5 September 2014 diff hist +15 Electroporation of A1 cells
- 08:43, 5 September 2014 diff hist +11 Electroporation of A1 cells
- 08:41, 5 September 2014 diff hist +14 Electroporation of A1 cells
- 08:36, 5 September 2014 diff hist 0 Electroporation of A1 cells
- 08:35, 5 September 2014 diff hist +7 Electroporation of A1 cells
- 08:16, 5 September 2014 diff hist -3 Electroporation of A1 cells
- 08:14, 5 September 2014 diff hist -4 Electroporation of A1 cells
- 08:12, 5 September 2014 diff hist +100 Electroporation of A1 cells
- 07:26, 5 September 2014 diff hist +3 Electroporation of A1 cells
- 07:24, 5 September 2014 diff hist 0 Electroporation of A1 cells
- 07:23, 5 September 2014 diff hist -5 Electroporation of A1 cells
- 07:22, 5 September 2014 diff hist -13 Electroporation of A1 cells
- 07:20, 5 September 2014 diff hist +12 Electroporation of A1 cells
- 07:19, 5 September 2014 diff hist +4 Electroporation of A1 cells
- 07:18, 5 September 2014 diff hist +899 N Electroporation of A1 cells Created page with "==Materials:== Ice 4 mM cuvettes, N+1 cuvettes Steinberg's, at 4 C. Square pulse electroporator (we use the BTX 830 Squarporator) 1) Put cuvettes on ice, and cool down..."
- 09:33, 3 September 2014 diff hist 0 Thawing frozen C2C12 cells
- 09:31, 3 September 2014 diff hist +711 N Thawing frozen C2C12 cells Created page with "==Materials:== - frozen C2C12 cells - HS for C2C12 cells - pipettes and tips - FALCON tube 15 ml - Cell culture flask ==Procedure:== - heat the HS up to 37ºC - pipette 14 ml..."
- 09:09, 3 September 2014 diff hist 0 Myotube preparation of C2C12 cells
- 09:05, 3 September 2014 diff hist 0 Myotube preparation of C2C12 cells
- 09:03, 3 September 2014 diff hist +8 Myotube preparation of C2C12 cells
- 09:02, 3 September 2014 diff hist +2,773 N Myotube preparation of C2C12 cells Created page with "Materials for 1 x 96 well plate: - DMEM (3.5 ml) –purely - Fibronectin (final diluted concentration should be 20 ng/µl)) - 1x 96 well plate - 2% Horse Serum – Low Serum (..."
- 07:17, 29 August 2014 diff hist -6 Whole mount ISH on axolotl tissue
- 07:15, 29 August 2014 diff hist -24 Whole mount ISH on axolotl tissue
- 07:14, 29 August 2014 diff hist +1 Whole mount ISH on axolotl tissue
- 07:14, 29 August 2014 diff hist +3 Whole mount ISH on axolotl tissue
- 07:13, 29 August 2014 diff hist +1 Whole mount ISH on axolotl tissue
- 07:12, 29 August 2014 diff hist +19 Whole mount ISH on axolotl tissue
- 07:08, 29 August 2014 diff hist +3 Whole mount ISH on axolotl tissue
- 07:06, 29 August 2014 diff hist +6 Whole mount ISH on axolotl tissue
- 07:04, 29 August 2014 diff hist -40 Whole mount ISH on axolotl tissue
- 07:00, 29 August 2014 diff hist +3,046 N Whole mount ISH on axolotl tissue Created page with "# fix embryo or tail blastema in MEMFA (0.1M MOPS pH7.4, 2mM EGTA, 1mM MgSO4, 3.7% formaldehyde) 2h to over night at room temperature # store at –20C in 100% MeOH or EtOH (p..."
- 10:28, 22 August 2014 diff hist +835 N Labelling Pax6 with Dig-NHS Created page with "2 June 2005 Pax6 AB is at 5 mg/ml, 1 ml 1) Dialyze 500 µl into PBS O/N then one change in morning 2 hours. 2) Spin antibody and check protein concentration by BioRad STD ..."
- 08:02, 22 August 2014 diff hist +22 Thawing 293FT cells
- 07:56, 22 August 2014 diff hist -9 Thawing 293FT cells
- 07:52, 22 August 2014 diff hist +846 N Thawing 293FT cells Created page with "→ thaw the cells in prewarmed , complete medium without Geneticin materials: - a vial of frozen cells from the liquid nitrogen - 37ºC waterbath - 75 cm2 flask - HS for 293..."
- 09:43, 15 August 2014 diff hist +6 G418 medium preparation
- 09:41, 15 August 2014 diff hist +4 G418 medium preparation
- 09:39, 15 August 2014 diff hist +9 G418 medium preparation
- 09:37, 15 August 2014 diff hist +6 G418 medium preparation
- 09:35, 15 August 2014 diff hist +10 G418 medium preparation
- 09:33, 15 August 2014 diff hist +6 G418 medium preparation
- 09:30, 15 August 2014 diff hist +878 N G418 medium preparation Created page with "'''Materials:''' - 2x 15 ml FALCON tube - filter 0.22 µl - 5 ml syringe - DMEM - G418 '''Execution:''' 1 g of weight has a total concentration from 0,751 g How much of DMEM ..."
- 08:01, 11 August 2014 diff hist -11 Cardiomyocyte Preparation
- 08:00, 11 August 2014 diff hist +15 Cardiomyocyte Preparation
- 08:00, 11 August 2014 diff hist -4 Cardiomyocyte Preparation
- 07:59, 11 August 2014 diff hist 0 Cardiomyocyte Preparation
- 07:57, 11 August 2014 diff hist 0 Cardiomyocyte Preparation
- 07:55, 11 August 2014 diff hist -15 Cardiomyocyte Preparation
- 07:40, 11 August 2014 diff hist +1,449 N Cardiomyocyte Preparation Created page with "Bettencourt-Dias & Laube F combined 1) Collect hearts in 70% Leibovitz-15 (L15) medium (AL15) w/ pen-strep 2) Wash them once in 70% Leibovitz-15 (L15) medium (AL15) w/ pen-st..."
- 07:55, 6 August 2014 diff hist -1,895 Passaging A1 cell Blanked the page
- 07:53, 6 August 2014 diff hist +1,895 N A1 cell passaging myotube Created page with "<h4>For passaging 3 flasks of A1 cells:</h4> <li>1 flask into 3 flasks and <li>2 flasks into 2 x 10 cm plates (for making myotubes) <h3>Materials :</h3> <li>HS AMEM (see add..."
- 11:35, 4 August 2014 diff hist -1 Thawing (XB10) hybridoma cells
- 11:33, 4 August 2014 diff hist +1,150 Thawing (XB10) hybridoma cells
- 11:30, 4 August 2014 diff hist +15 Thawing (XB10) hybridoma cells
- 11:29, 4 August 2014 diff hist +427 Thawing (XB10) hybridoma cells
- 11:25, 4 August 2014 diff hist +14 N Thawing (XB10) hybridoma cells Created page with "==Materials:=="
- 11:23, 4 August 2014 diff hist +718 Hybridoma cells in SFX media
- 11:16, 4 August 2014 diff hist +201 N Hybridoma cells in SFX media Created page with "==Materials:== <li>5 ml, 10 ml, 20 ml pipettes <li>Serum free media( 400 mls SF media + 1% antibiotics 4 mls P/S and 4 mls Glutamine <li>2 blue cap..."
- 10:34, 2 July 2014 diff hist -1 Passaging A1 cell
- 10:33, 2 July 2014 diff hist -21 Passaging A1 cell
- 10:23, 2 July 2014 diff hist +21 Passaging A1 cell
- 09:12, 2 July 2014 diff hist -26 Passaging A1 cell
- 09:07, 2 July 2014 diff hist -5 Passaging A1 cell
- 09:04, 2 July 2014 diff hist +32 Passaging A1 cell
- 08:58, 2 July 2014 diff hist +9 Passaging A1 cell
- 08:57, 2 July 2014 diff hist +44 Passaging A1 cell
- 08:55, 2 July 2014 diff hist -86 Passaging A1 cell
- 08:52, 2 July 2014 diff hist +8 Passaging A1 cell
- 08:49, 2 July 2014 diff hist +9 Passaging A1 cell
- 08:41, 2 July 2014 diff hist +1 Passaging A1 cell
- 08:38, 2 July 2014 diff hist +1,910 N Passaging A1 cell Created page with "For passaging 3 flasks of A1 cells: 1 flask into 3 flasks and 2 flasks into 2 x10 cm plates (for making myotubes) Materials : HS AMEM (see additional protocol for recipe) ..."
- 09:34, 20 June 2014 diff hist +42 Letter to Froppier (French)
- 09:32, 20 June 2014 diff hist -42 Letter to Froppier (French)
- 09:30, 20 June 2014 diff hist +70 Letter to Froppier (French)
- 09:12, 20 June 2014 diff hist -9 Letter to Froppier (French)
- 09:08, 20 June 2014 diff hist -2 Letter to Froppier (French)
- 10:01, 18 June 2014 diff hist 0 Letter to Froppier (French)
- 10:00, 18 June 2014 diff hist 0 Letter to Froppier (French)
- 10:00, 18 June 2014 diff hist +1 Letter to Froppier (French)
- 09:57, 18 June 2014 diff hist +2 Letter to Froppier (French)
- 09:56, 18 June 2014 diff hist -8 Letter to Froppier (French)
- 09:56, 18 June 2014 diff hist +43 Letter to Froppier (French)
- 09:55, 18 June 2014 diff hist +20 Letter to Froppier (French)
- 09:53, 18 June 2014 diff hist +30 Letter to Froppier (French)
- 09:50, 18 June 2014 diff hist 0 Letter to Froppier (French)
- 09:49, 18 June 2014 diff hist +53 Letter to Froppier (French)
- 09:44, 18 June 2014 diff hist +3 Letter to Froppier (French)
- 09:42, 18 June 2014 diff hist -1 Letter to Froppier (French)
- 09:41, 18 June 2014 diff hist +89 Letter to Froppier (French)
- 09:30, 18 June 2014 diff hist +35 Letter to Froppier (French)
- 09:19, 18 June 2014 diff hist +733 N Letter to Froppier (French) Created page with "Max-Planck Institute Of Molecular Cell Biology and Genetics Pfotenhauerstrasse 108 01307 Dresden GERMANY G. FROPPIER Responsable Scientifique Elevage Scientifique des Dombes ..."
- 08:20, 11 June 2014 diff hist -2 BrdU and myosin staining →Materials:
- 08:19, 11 June 2014 diff hist -4 BrdU and myosin staining →Materials:
- 08:18, 11 June 2014 diff hist -6 BrdU and myosin staining →staining cells:
- 08:17, 11 June 2014 diff hist +20 BrdU and myosin staining →For staining cells:
- 08:15, 11 June 2014 diff hist +19 BrdU and myosin staining →Materials:
- 08:09, 11 June 2014 diff hist -15 BrdU and myosin staining
- 08:01, 11 June 2014 diff hist -14 BrdU and myosin staining
- 07:59, 11 June 2014 diff hist -7 BrdU and myosin staining
- 07:56, 11 June 2014 diff hist -2 BrdU and myosin staining
- 07:55, 11 June 2014 diff hist +3,204 N BrdU and myosin staining Created page with "== Fixing Cells: == 1 thaw 6% PFA in 37 C waterbath , place on ice 2 put 5 ml PBS + 5 ml 6% PFA in tray “PFA” , mix well 3 put 0.01% BSA/PBS into tray “BSA” ..."
- 08:27, 7 May 2014 diff hist +943 N Antibody staining Created page with "* Cut the tail and fix it in fresh 4% PFA in phosphate buffer from few hours to o/n always at 4C * Wash in PBS 1 hour at room temperature, put tail in 30% sucrose Put in OCT s..."
- 09:00, 2 May 2014 diff hist +252 BrdU labelling of A1 cells in 96-well plate
- 08:56, 2 May 2014 diff hist +102 N BrdU labelling of A1 cells in 96-well plate Created page with "== Materials: == * 5ml tube * SF MEM * BrDU drawer no. 3 * Multipipette * Pipette 100ul * Pipette 5ml"
- 08:46, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:45, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:36, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:34, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:32, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:29, 28 April 2014 diff hist +805 Passaging C2C12 cells
- 08:28, 28 April 2014 diff hist +17 Passaging C2C12 cells
- 06:58, 28 April 2014 diff hist +143 N Passaging C2C12 cells Created page with "== Materials: == * 1x 15ml FALCON tube * PBS w/o Ca2+ and Mg2+ * TE in PBS (4 ml TE + 36 ml PBS) * HS for C2C12 cells * pipettes * 75 cm2 flask"
- 09:32, 24 March 2014 diff hist +768 Purifying IgG1 from Hybridoma Supernatant
- 09:27, 24 March 2014 diff hist -45 Purifying IgG1 from Hybridoma Supernatant
- 09:25, 24 March 2014 diff hist +313 N Purifying IgG1 from Hybridoma Supernatant Created page with "<h3>Materials:</h3> <li>Supernatant (100-200 ml)</li> <li>Peristaltic pump</li> <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml)</li> <li>PBS (100 ml)</li> <li>10x ..."
- 12:48, 18 March 2014 diff hist -6 Overview →Protocols
- 12:45, 18 March 2014 diff hist +6 Overview →Antibody
- 12:42, 18 March 2014 diff hist +133 Overview →Protocols
- 12:19, 18 March 2014 diff hist +861 Labeled AB staining
- 12:12, 18 March 2014 diff hist +315 Labeled AB staining
- 12:06, 18 March 2014 diff hist +38 N Labeled AB staining Created page with "<h4>Pax7 + cy3 + NeuN – cy5Fab</h4>"
- 11:56, 18 March 2014 diff hist +50 Overview →Protocols
- 12:21, 10 March 2014 diff hist +10 RNA formaldehyde gels
- 12:17, 10 March 2014 diff hist +649 RNA formaldehyde gels
- 12:11, 10 March 2014 diff hist +205 RNA formaldehyde gels
- 12:06, 10 March 2014 diff hist +5 RNA formaldehyde gels
- 12:05, 10 March 2014 diff hist +248 N RNA formaldehyde gels Created page with "<h3>Prepare gel box: <li>Clean gel box and combs etc with detergent, then dionized water. <li>Then incubate the gel box and components in 10% H2O2 (hydrogen peroxide, stored i..."
- 12:01, 10 March 2014 diff hist +57 Overview →Molecular biology
- 08:52, 10 March 2014 diff hist -45 RNA Extraction from axolotl tissue
- 08:49, 10 March 2014 diff hist +755 RNA Extraction from axolotl tissue
- 08:43, 10 March 2014 diff hist +41 RNA Extraction from axolotl tissue
- 08:39, 10 March 2014 diff hist +56 RNA Extraction from axolotl tissue
- 08:34, 10 March 2014 diff hist +239 RNA Extraction from axolotl tissue
- 08:20, 10 March 2014 diff hist -1 RNA Extraction from axolotl tissue
- 08:20, 10 March 2014 diff hist +8 RNA Extraction from axolotl tissue
- 08:18, 10 March 2014 diff hist +12 N RNA Extraction from axolotl tissue Created page with " Materials:"
- 12:36, 6 March 2014 diff hist -5 Overview →Molecular biology
- 12:27, 6 March 2014 diff hist -3 Overview →Molecular biology
- 12:22, 6 March 2014 diff hist +89 Overview →Molecular biology
- 12:10, 6 March 2014 diff hist -102 Overview →RNA
- 09:30, 6 March 2014 diff hist +93 RNA extraction from axolotl tissue →Procedure to extract total RNA: current
- 09:21, 6 March 2014 diff hist +12 RNA extraction from axolotl tissue →Procedure to extract total RNA:
- 09:20, 6 March 2014 diff hist +1,242 N RNA extraction from axolotl tissue Created page with "== RNA Extraction from axolotl tissue == === Materials: === <ul> <li>Trizol (Invitrogen)</li> <li>Chloroform</li> <li>Isopropanol</li> <li>RNase free water</li> <..."
- 08:35, 6 March 2014 diff hist +95 Overview →Protocols
- 11:37, 17 January 2013 diff hist +7 Overview →Artemia Hatching Preparation: Protocol for 15 Ltr.
- 11:35, 17 January 2013 diff hist -1 Overview →Artemia Hatching Preparation: Protocol for 15 Ltr.
- 11:34, 17 January 2013 diff hist +370 Overview →Artemia Hatching Preparation: Protocol for 15 Ltr.
- 11:29, 17 January 2013 diff hist +62 Overview →Artemia (Brine shrimp eggs)
- 11:10, 17 January 2013 diff hist +78 Overview
- 11:00, 17 January 2013 diff hist -37 Overview
- 10:59, 17 January 2013 diff hist +37 Overview