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- 08:07, 26 September 2014 diff hist +6 Passaging A1 cell
- 08:06, 26 September 2014 diff hist +787 Passaging A1 cell
- 07:44, 24 September 2014 diff hist +4 Thawing frozen A1 cells
- 07:43, 24 September 2014 diff hist 0 Thawing frozen A1 cells
- 07:37, 24 September 2014 diff hist +828 N Thawing frozen A1 cells Created page with "== Materials: == - Gelatin (warmed to 37ºC) - HS for A1 cells - 1x 15 ml FALCON tubes - Pipettes - 1x 175 cm2 flasks - frozen cells into liquid nitrogen == Procedure: == - p..."
- 08:45, 5 September 2014 diff hist +2 Electroporation of A1 cells
- 08:45, 5 September 2014 diff hist +15 Electroporation of A1 cells
- 08:43, 5 September 2014 diff hist +11 Electroporation of A1 cells
- 08:41, 5 September 2014 diff hist +14 Electroporation of A1 cells
- 08:36, 5 September 2014 diff hist 0 Electroporation of A1 cells
- 08:35, 5 September 2014 diff hist +7 Electroporation of A1 cells
- 08:16, 5 September 2014 diff hist -3 Electroporation of A1 cells
- 08:14, 5 September 2014 diff hist -4 Electroporation of A1 cells
- 08:12, 5 September 2014 diff hist +100 Electroporation of A1 cells
- 07:26, 5 September 2014 diff hist +3 Electroporation of A1 cells
- 07:24, 5 September 2014 diff hist 0 Electroporation of A1 cells
- 07:23, 5 September 2014 diff hist -5 Electroporation of A1 cells
- 07:22, 5 September 2014 diff hist -13 Electroporation of A1 cells
- 07:20, 5 September 2014 diff hist +12 Electroporation of A1 cells
- 07:19, 5 September 2014 diff hist +4 Electroporation of A1 cells
- 07:18, 5 September 2014 diff hist +899 N Electroporation of A1 cells Created page with "==Materials:== Ice 4 mM cuvettes, N+1 cuvettes Steinberg's, at 4 C. Square pulse electroporator (we use the BTX 830 Squarporator) 1) Put cuvettes on ice, and cool down..."
- 09:33, 3 September 2014 diff hist 0 Thawing frozen C2C12 cells
- 09:31, 3 September 2014 diff hist +711 N Thawing frozen C2C12 cells Created page with "==Materials:== - frozen C2C12 cells - HS for C2C12 cells - pipettes and tips - FALCON tube 15 ml - Cell culture flask ==Procedure:== - heat the HS up to 37ºC - pipette 14 ml..."
- 09:09, 3 September 2014 diff hist 0 Myotube preparation of C2C12 cells
- 09:05, 3 September 2014 diff hist 0 Myotube preparation of C2C12 cells
- 09:03, 3 September 2014 diff hist +8 Myotube preparation of C2C12 cells
- 09:02, 3 September 2014 diff hist +2,773 N Myotube preparation of C2C12 cells Created page with "Materials for 1 x 96 well plate: - DMEM (3.5 ml) –purely - Fibronectin (final diluted concentration should be 20 ng/µl)) - 1x 96 well plate - 2% Horse Serum – Low Serum (..."
- 07:17, 29 August 2014 diff hist -6 Whole mount ISH on axolotl tissue
- 07:15, 29 August 2014 diff hist -24 Whole mount ISH on axolotl tissue
- 07:14, 29 August 2014 diff hist +1 Whole mount ISH on axolotl tissue
- 07:14, 29 August 2014 diff hist +3 Whole mount ISH on axolotl tissue
- 07:13, 29 August 2014 diff hist +1 Whole mount ISH on axolotl tissue
- 07:12, 29 August 2014 diff hist +19 Whole mount ISH on axolotl tissue
- 07:08, 29 August 2014 diff hist +3 Whole mount ISH on axolotl tissue
- 07:06, 29 August 2014 diff hist +6 Whole mount ISH on axolotl tissue
- 07:04, 29 August 2014 diff hist -40 Whole mount ISH on axolotl tissue
- 07:00, 29 August 2014 diff hist +3,046 N Whole mount ISH on axolotl tissue Created page with "# fix embryo or tail blastema in MEMFA (0.1M MOPS pH7.4, 2mM EGTA, 1mM MgSO4, 3.7% formaldehyde) 2h to over night at room temperature # store at –20C in 100% MeOH or EtOH (p..."
- 10:28, 22 August 2014 diff hist +835 N Labelling Pax6 with Dig-NHS Created page with "2 June 2005 Pax6 AB is at 5 mg/ml, 1 ml 1) Dialyze 500 µl into PBS O/N then one change in morning 2 hours. 2) Spin antibody and check protein concentration by BioRad STD ..."
- 08:02, 22 August 2014 diff hist +22 Thawing 293FT cells
- 07:56, 22 August 2014 diff hist -9 Thawing 293FT cells
- 07:52, 22 August 2014 diff hist +846 N Thawing 293FT cells Created page with "→ thaw the cells in prewarmed , complete medium without Geneticin materials: - a vial of frozen cells from the liquid nitrogen - 37ºC waterbath - 75 cm2 flask - HS for 293..."
- 09:43, 15 August 2014 diff hist +6 G418 medium preparation
- 09:41, 15 August 2014 diff hist +4 G418 medium preparation
- 09:39, 15 August 2014 diff hist +9 G418 medium preparation
- 09:37, 15 August 2014 diff hist +6 G418 medium preparation
- 09:35, 15 August 2014 diff hist +10 G418 medium preparation
- 09:33, 15 August 2014 diff hist +6 G418 medium preparation
- 09:30, 15 August 2014 diff hist +878 N G418 medium preparation Created page with "'''Materials:''' - 2x 15 ml FALCON tube - filter 0.22 µl - 5 ml syringe - DMEM - G418 '''Execution:''' 1 g of weight has a total concentration from 0,751 g How much of DMEM ..."
- 08:01, 11 August 2014 diff hist -11 Cardiomyocyte Preparation
- 08:00, 11 August 2014 diff hist +15 Cardiomyocyte Preparation