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- 08:27, 7 May 2014 diff hist +943 N Antibody staining Created page with "* Cut the tail and fix it in fresh 4% PFA in phosphate buffer from few hours to o/n always at 4C * Wash in PBS 1 hour at room temperature, put tail in 30% sucrose Put in OCT s..."
- 09:00, 2 May 2014 diff hist +252 BrdU labelling of A1 cells in 96-well plate
- 08:56, 2 May 2014 diff hist +102 N BrdU labelling of A1 cells in 96-well plate Created page with "== Materials: == * 5ml tube * SF MEM * BrDU drawer no. 3 * Multipipette * Pipette 100ul * Pipette 5ml"
- 08:46, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:45, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:36, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:34, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:32, 28 April 2014 diff hist 0 Passaging C2C12 cells
- 08:29, 28 April 2014 diff hist +805 Passaging C2C12 cells
- 08:28, 28 April 2014 diff hist +17 Passaging C2C12 cells
- 06:58, 28 April 2014 diff hist +143 N Passaging C2C12 cells Created page with "== Materials: == * 1x 15ml FALCON tube * PBS w/o Ca2+ and Mg2+ * TE in PBS (4 ml TE + 36 ml PBS) * HS for C2C12 cells * pipettes * 75 cm2 flask"
- 09:32, 24 March 2014 diff hist +768 Purifying IgG1 from Hybridoma Supernatant
- 09:27, 24 March 2014 diff hist -45 Purifying IgG1 from Hybridoma Supernatant
- 09:25, 24 March 2014 diff hist +313 N Purifying IgG1 from Hybridoma Supernatant Created page with "<h3>Materials:</h3> <li>Supernatant (100-200 ml)</li> <li>Peristaltic pump</li> <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml)</li> <li>PBS (100 ml)</li> <li>10x ..."
- 12:48, 18 March 2014 diff hist -6 Overview →Protocols
- 12:45, 18 March 2014 diff hist +6 Overview →Antibody
- 12:42, 18 March 2014 diff hist +133 Overview →Protocols
- 12:19, 18 March 2014 diff hist +861 Labeled AB staining
- 12:12, 18 March 2014 diff hist +315 Labeled AB staining
- 12:06, 18 March 2014 diff hist +38 N Labeled AB staining Created page with "<h4>Pax7 + cy3 + NeuN – cy5Fab</h4>"
- 11:56, 18 March 2014 diff hist +50 Overview →Protocols
- 12:21, 10 March 2014 diff hist +10 RNA formaldehyde gels
- 12:17, 10 March 2014 diff hist +649 RNA formaldehyde gels
- 12:11, 10 March 2014 diff hist +205 RNA formaldehyde gels
- 12:06, 10 March 2014 diff hist +5 RNA formaldehyde gels
- 12:05, 10 March 2014 diff hist +248 N RNA formaldehyde gels Created page with "<h3>Prepare gel box: <li>Clean gel box and combs etc with detergent, then dionized water. <li>Then incubate the gel box and components in 10% H2O2 (hydrogen peroxide, stored i..."
- 12:01, 10 March 2014 diff hist +57 Overview →Molecular biology
- 08:52, 10 March 2014 diff hist -45 RNA Extraction from axolotl tissue
- 08:49, 10 March 2014 diff hist +755 RNA Extraction from axolotl tissue
- 08:43, 10 March 2014 diff hist +41 RNA Extraction from axolotl tissue
- 08:39, 10 March 2014 diff hist +56 RNA Extraction from axolotl tissue
- 08:34, 10 March 2014 diff hist +239 RNA Extraction from axolotl tissue
- 08:20, 10 March 2014 diff hist -1 RNA Extraction from axolotl tissue
- 08:20, 10 March 2014 diff hist +8 RNA Extraction from axolotl tissue
- 08:18, 10 March 2014 diff hist +12 N RNA Extraction from axolotl tissue Created page with " Materials:"
- 12:36, 6 March 2014 diff hist -5 Overview →Molecular biology
- 12:27, 6 March 2014 diff hist -3 Overview →Molecular biology
- 12:22, 6 March 2014 diff hist +89 Overview →Molecular biology
- 12:10, 6 March 2014 diff hist -102 Overview →RNA
- 09:30, 6 March 2014 diff hist +93 RNA extraction from axolotl tissue →Procedure to extract total RNA: current
- 09:21, 6 March 2014 diff hist +12 RNA extraction from axolotl tissue →Procedure to extract total RNA:
- 09:20, 6 March 2014 diff hist +1,242 N RNA extraction from axolotl tissue Created page with "== RNA Extraction from axolotl tissue == === Materials: === <ul> <li>Trizol (Invitrogen)</li> <li>Chloroform</li> <li>Isopropanol</li> <li>RNase free water</li> <..."
- 08:35, 6 March 2014 diff hist +95 Overview →Protocols
- 11:37, 17 January 2013 diff hist +7 Overview →Artemia Hatching Preparation: Protocol for 15 Ltr.
- 11:35, 17 January 2013 diff hist -1 Overview →Artemia Hatching Preparation: Protocol for 15 Ltr.
- 11:34, 17 January 2013 diff hist +370 Overview →Artemia Hatching Preparation: Protocol for 15 Ltr.
- 11:29, 17 January 2013 diff hist +62 Overview →Artemia (Brine shrimp eggs)
- 11:10, 17 January 2013 diff hist +78 Overview
- 11:00, 17 January 2013 diff hist -37 Overview
- 10:59, 17 January 2013 diff hist +37 Overview