User contributions
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- 08:18, 29 June 2015 (diff | hist) . . (-6) . . Anti-BrDU Antibody labeling (→Procedure) (current)
- 08:16, 29 June 2015 (diff | hist) . . (+8) . . Anti-BrDU Antibody labeling (→Material)
- 08:13, 29 June 2015 (diff | hist) . . (+6) . . Anti-BrDU Antibody labeling (→Material)
- 08:13, 29 June 2015 (diff | hist) . . (-1) . . Anti-BrDU Antibody labeling
- 08:10, 29 June 2015 (diff | hist) . . (+1,562) . . N Anti-BrDU Antibody labeling (Created page with "Materials: Centricon-100 Rhodamine N-hydroxy-succiniymidyl ester (stored at -20) DMSO Antibody in 0.1M Borate buffer pH 8.8 NAP desalting columns or dialysis membrane. 0....")
- 08:09, 29 June 2015 (diff | hist) . . (+41) . . Overview (→Monoclonal Antibodies:)
- 08:06, 29 June 2015 (diff | hist) . . (-60) . . Overview (→Polyclonal Antibodies:)
- 07:57, 29 June 2015 (diff | hist) . . (-142) . . Address for making polyclonal antibodies (current)
- 07:57, 29 June 2015 (diff | hist) . . (+101) . . Address for making polyclonal antibodies
- 07:54, 29 June 2015 (diff | hist) . . (+28) . . Address for making polyclonal antibodies
- 07:52, 29 June 2015 (diff | hist) . . (+4) . . Address for making polyclonal antibodies
- 07:51, 29 June 2015 (diff | hist) . . (+2) . . Address for making polyclonal antibodies
- 07:51, 29 June 2015 (diff | hist) . . (+10) . . Address for making polyclonal antibodies
- 07:49, 29 June 2015 (diff | hist) . . (-2) . . Address for making polyclonal antibodies
- 07:48, 29 June 2015 (diff | hist) . . (0) . . Address for making polyclonal antibodies
- 07:48, 29 June 2015 (diff | hist) . . (+2) . . Address for making polyclonal antibodies
- 07:47, 29 June 2015 (diff | hist) . . (+97) . . N Address for making polyclonal antibodies (Created page with "Elevage Scientifique Des Dombes Romans 01400 chatillon sur chalaronne France Tel: 04.74.55.34.97")
- 07:45, 29 June 2015 (diff | hist) . . (-2) . . Overview (→Polyclonal Antibodies:)
- 14:01, 24 June 2015 (diff | hist) . . (-1) . . Baculovirus titration (current)
- 14:00, 24 June 2015 (diff | hist) . . (+30) . . Baculovirus titration
- 13:56, 24 June 2015 (diff | hist) . . (-414) . . Baculovirus titration
- 13:50, 24 June 2015 (diff | hist) . . (+3,084) . . N Baculovirus titration (Created page with "This protocol is not optimized yet. For example, adding SF+ cells before or after the virus seems to make a big difference… more troubleshooting is needed. Serial dilute th...")
- 08:31, 24 June 2015 (diff | hist) . . (-6) . . Overview (→Molecular biology)
- 08:30, 24 June 2015 (diff | hist) . . (-4) . . Overview (→Molecular biology)
- 08:29, 24 June 2015 (diff | hist) . . (+6) . . Overview (→Molecular biology)
- 08:28, 24 June 2015 (diff | hist) . . (-2) . . Overview (→Molecular biology)
- 08:25, 24 June 2015 (diff | hist) . . (-9) . . Overview (→Molecular biology)
- 08:05, 24 June 2015 (diff | hist) . . (-4) . . Neurosphere culture (current)
- 08:02, 24 June 2015 (diff | hist) . . (+35) . . Neurosphere culture
- 08:00, 24 June 2015 (diff | hist) . . (+12) . . Neurosphere culture
- 07:59, 24 June 2015 (diff | hist) . . (+6) . . Neurosphere culture
- 07:58, 24 June 2015 (diff | hist) . . (-3) . . Neurosphere culture
- 07:54, 24 June 2015 (diff | hist) . . (+6) . . Neurosphere culture
- 07:53, 24 June 2015 (diff | hist) . . (-12) . . Neurosphere culture
- 07:52, 24 June 2015 (diff | hist) . . (-24) . . Neurosphere culture
- 07:36, 24 June 2015 (diff | hist) . . (+1,685) . . N Neurosphere culture (Created page with "Neurosphere Culture of SC Dissect SC from 5cm animals (GFP) Place SC into L15 medium (Gibco Cat. No: 31415-029) Remove meninges and cut SC in smaller pieces Transfer SC into ...")
- 08:52, 19 June 2015 (diff | hist) . . (0) . . RNA Extraction from axolotl tissue (→Materials) (current)
- 08:51, 19 June 2015 (diff | hist) . . (-1) . . Labeled AB staining (→Materials) (current)
- 08:49, 19 June 2015 (diff | hist) . . (-1) . . Labelling myosin antibody (→Materials) (current)
- 08:48, 19 June 2015 (diff | hist) . . (-1) . . Purifying IgG1 from Hybridoma Supernatant (→Materials) (current)
- 08:47, 19 June 2015 (diff | hist) . . (-1) . . ISH on axolotl tissue sections (→Materials) (current)
- 08:46, 19 June 2015 (diff | hist) . . (+27) . . Double Immunostaining via antigen retrieval (→Procedure) (current)
- 14:16, 15 June 2015 (diff | hist) . . (-1) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5)) (current)
- 14:14, 15 June 2015 (diff | hist) . . (-2) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 14:13, 15 June 2015 (diff | hist) . . (+18) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 14:06, 15 June 2015 (diff | hist) . . (-11) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 14:01, 15 June 2015 (diff | hist) . . (+32) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 13:58, 15 June 2015 (diff | hist) . . (+30) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 13:56, 15 June 2015 (diff | hist) . . (+37) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 13:52, 15 June 2015 (diff | hist) . . (-2) . . Hybridoma cells clones (→Antibody Staining (step 2 & 5))
- 13:52, 15 June 2015 (diff | hist) . . (-12) . . Hybridoma cells clones (→ELISA (step 1 & 4))
- 14:37, 8 June 2015 (diff | hist) . . (-2) . . Hybridoma cells clones
- 14:35, 8 June 2015 (diff | hist) . . (-1) . . Hybridoma cells in SFX media (current)
- 14:34, 8 June 2015 (diff | hist) . . (-1) . . Preparation of Freezing media for C2C12 cells (current)
- 14:34, 8 June 2015 (diff | hist) . . (-1) . . Preparation of High Serum for C2C12 cells (current)
- 14:33, 8 June 2015 (diff | hist) . . (-1) . . Preparation of Low Serum for C2C12 cells (current)
- 14:33, 8 June 2015 (diff | hist) . . (0) . . Electroporation of C2C12 cells (current)
- 14:32, 8 June 2015 (diff | hist) . . (-3) . . Cloning of C2C12 cells (current)
- 14:32, 8 June 2015 (diff | hist) . . (-1) . . C2C12 cell transfection with FuGene (current)
- 14:31, 8 June 2015 (diff | hist) . . (-1) . . Myotube preparation of C2C12 cells (current)
- 14:31, 8 June 2015 (diff | hist) . . (-1) . . Passaging C2C12 cells (current)
- 14:30, 8 June 2015 (diff | hist) . . (-1) . . Freezing of C2C12 cells (current)
- 14:30, 8 June 2015 (diff | hist) . . (-1) . . Thawing frozen C2C12 cells (current)
- 14:29, 8 June 2015 (diff | hist) . . (-1) . . Thawing (XB10) hybridoma cells (current)
- 14:29, 8 June 2015 (diff | hist) . . (-1) . . MESC culture (current)
- 14:28, 8 June 2015 (diff | hist) . . (-1) . . Preparation of Freezing media for A1 cells (current)
- 14:28, 8 June 2015 (diff | hist) . . (-1) . . Preparation of High Serum for A1 cells (→Materials) (current)
- 14:26, 8 June 2015 (diff | hist) . . (-1) . . BrdU and myosin staining (current)
- 14:26, 8 June 2015 (diff | hist) . . (-1) . . Preparation of Low Serum for A1 cells (current)
- 14:25, 8 June 2015 (diff | hist) . . (-1) . . BrdU labelling of A1 cells in 96-well plate (current)
- 14:25, 8 June 2015 (diff | hist) . . (-1) . . Electroporation of A1 cells (current)
- 14:24, 8 June 2015 (diff | hist) . . (-1) . . A1 cell transfection with FuGene (current)
- 14:24, 8 June 2015 (diff | hist) . . (-1) . . Modified myotube prep (current)
- 14:23, 8 June 2015 (diff | hist) . . (-1) . . A1 cell passaging myotube (current)
- 14:23, 8 June 2015 (diff | hist) . . (0) . . Passaging A1 cell (current)
- 14:21, 8 June 2015 (diff | hist) . . (-1) . . Passaging A1 cell
- 14:20, 8 June 2015 (diff | hist) . . (-1) . . Freezing of A1 cells (current)
- 14:20, 8 June 2015 (diff | hist) . . (-1) . . Thawing frozen A1 cells (current)
- 14:19, 8 June 2015 (diff | hist) . . (-1) . . G418 medium preparation (current)
- 14:18, 8 June 2015 (diff | hist) . . (-1) . . Thawing 293FT cells (current)
- 14:18, 8 June 2015 (diff | hist) . . (-1) . . Myoblasts electroporation (current)
- 14:17, 8 June 2015 (diff | hist) . . (-1) . . Adhesive cell culture (current)
- 14:17, 8 June 2015 (diff | hist) . . (+1) . . Adhesive cell culture
- 14:15, 8 June 2015 (diff | hist) . . (-2) . . Adhesive cell culture
- 14:12, 8 June 2015 (diff | hist) . . (-2) . . Adhesive cell culture
- 14:10, 8 June 2015 (diff | hist) . . (+28) . . Adhesive cell culture
- 14:06, 8 June 2015 (diff | hist) . . (+21) . . Adhesive cell culture
- 14:03, 8 June 2015 (diff | hist) . . (+17) . . Adhesive cell culture
- 13:55, 8 June 2015 (diff | hist) . . (+3,049) . . N Adhesive cell culture (Created page with "ECM coating and composition of culture media These steps for preparation were carried out under sterile terms in cell culture room at clean bench. Gelatine: Gelatine (Sigma,...")
- 08:49, 1 June 2015 (diff | hist) . . (0) . . TSA Staining on axolotl-tissue (current)
- 08:48, 1 June 2015 (diff | hist) . . (+6) . . TSA Staining on axolotl-tissue
- 08:40, 1 June 2015 (diff | hist) . . (-12) . . TSA Staining on axolotl-tissue
- 08:39, 1 June 2015 (diff | hist) . . (0) . . TSA Staining on axolotl-tissue
- 08:37, 1 June 2015 (diff | hist) . . (0) . . TSA Staining on axolotl-tissue
- 08:35, 1 June 2015 (diff | hist) . . (0) . . TSA Staining on axolotl-tissue
- 08:34, 1 June 2015 (diff | hist) . . (+12) . . TSA Staining on axolotl-tissue
- 08:28, 1 June 2015 (diff | hist) . . (-20) . . TSA Staining on axolotl-tissue
- 08:24, 1 June 2015 (diff | hist) . . (-2) . . TSA Staining on axolotl-tissue
- 08:23, 1 June 2015 (diff | hist) . . (+1) . . TSA Staining on axolotl-tissue
- 08:22, 1 June 2015 (diff | hist) . . (-6) . . TSA Staining on axolotl-tissue
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