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- 10:28, 22 August 2014 diff hist +835 N Labelling Pax6 with Dig-NHS Created page with "2 June 2005 Pax6 AB is at 5 mg/ml, 1 ml 1) Dialyze 500 µl into PBS O/N then one change in morning 2 hours. 2) Spin antibody and check protein concentration by BioRad STD ..."
- 08:02, 22 August 2014 diff hist +22 Thawing 293FT cells
- 07:56, 22 August 2014 diff hist -9 Thawing 293FT cells
- 07:52, 22 August 2014 diff hist +846 N Thawing 293FT cells Created page with "→ thaw the cells in prewarmed , complete medium without Geneticin materials: - a vial of frozen cells from the liquid nitrogen - 37ºC waterbath - 75 cm2 flask - HS for 293..."
- 09:43, 15 August 2014 diff hist +6 G418 medium preparation
- 09:41, 15 August 2014 diff hist +4 G418 medium preparation
- 09:39, 15 August 2014 diff hist +9 G418 medium preparation
- 09:37, 15 August 2014 diff hist +6 G418 medium preparation
- 09:35, 15 August 2014 diff hist +10 G418 medium preparation
- 09:33, 15 August 2014 diff hist +6 G418 medium preparation
- 09:30, 15 August 2014 diff hist +878 N G418 medium preparation Created page with "'''Materials:''' - 2x 15 ml FALCON tube - filter 0.22 µl - 5 ml syringe - DMEM - G418 '''Execution:''' 1 g of weight has a total concentration from 0,751 g How much of DMEM ..."
- 08:01, 11 August 2014 diff hist -11 Cardiomyocyte Preparation
- 08:00, 11 August 2014 diff hist +15 Cardiomyocyte Preparation
- 08:00, 11 August 2014 diff hist -4 Cardiomyocyte Preparation
- 07:59, 11 August 2014 diff hist 0 Cardiomyocyte Preparation
- 07:57, 11 August 2014 diff hist 0 Cardiomyocyte Preparation
- 07:55, 11 August 2014 diff hist -15 Cardiomyocyte Preparation
- 07:40, 11 August 2014 diff hist +1,449 N Cardiomyocyte Preparation Created page with "Bettencourt-Dias & Laube F combined 1) Collect hearts in 70% Leibovitz-15 (L15) medium (AL15) w/ pen-strep 2) Wash them once in 70% Leibovitz-15 (L15) medium (AL15) w/ pen-st..."
- 07:55, 6 August 2014 diff hist -1,895 Passaging A1 cell Blanked the page
- 07:53, 6 August 2014 diff hist +1,895 N A1 cell passaging myotube Created page with "<h4>For passaging 3 flasks of A1 cells:</h4> <li>1 flask into 3 flasks and <li>2 flasks into 2 x 10 cm plates (for making myotubes) <h3>Materials :</h3> <li>HS AMEM (see add..."