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- 07:44, 20 October 2014 diff hist +115 ISH on axolotl tissue sections
- 07:40, 20 October 2014 diff hist -6 ISH on axolotl tissue sections
- 07:39, 20 October 2014 diff hist -2 ISH on axolotl tissue sections
- 07:38, 20 October 2014 diff hist -4 ISH on axolotl tissue sections
- 07:34, 20 October 2014 diff hist +1 ISH on axolotl tissue sections
- 07:29, 20 October 2014 diff hist +1 ISH on axolotl tissue sections
- 07:26, 20 October 2014 diff hist +12 ISH on axolotl tissue sections
- 11:57, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:57, 13 October 2014 diff hist +15 ISH on axolotl tissue sections
- 11:55, 13 October 2014 diff hist +8 ISH on axolotl tissue sections
- 11:48, 13 October 2014 diff hist +3 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist -7 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist +13 ISH on axolotl tissue sections
- 11:47, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:46, 13 October 2014 diff hist +18 ISH on axolotl tissue sections
- 11:45, 13 October 2014 diff hist 0 ISH on axolotl tissue sections
- 11:44, 13 October 2014 diff hist -8 ISH on axolotl tissue sections
- 11:42, 13 October 2014 diff hist +2 ISH on axolotl tissue sections
- 11:41, 13 October 2014 diff hist -1 ISH on axolotl tissue sections
- 11:40, 13 October 2014 diff hist -5 ISH on axolotl tissue sections
- 11:39, 13 October 2014 diff hist -5 ISH on axolotl tissue sections
- 11:35, 13 October 2014 diff hist +5,086 N ISH on axolotl tissue sections Created page with "== Probe preparation == 1. Mix the following at RT: 10x transcription buffer 2 µl dig Nucleotide mix 2 µl Linearized plasmid x µl (1µg) RNase inhibito..."
- 08:02, 10 October 2014 diff hist +1 Modified myotube prep
- 08:01, 10 October 2014 diff hist +7 Modified myotube prep
- 08:00, 10 October 2014 diff hist +4 Modified myotube prep
- 07:59, 10 October 2014 diff hist +1 Cardiomyocyte Preparation
- 07:58, 10 October 2014 diff hist -3 Cardiomyocyte Preparation
- 07:56, 10 October 2014 diff hist -1 Cardiomyocyte Preparation
- 07:54, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:54, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:53, 10 October 2014 diff hist -2 Cardiomyocyte Preparation
- 07:52, 10 October 2014 diff hist +4 Cardiomyocyte Preparation
- 07:51, 10 October 2014 diff hist 0 Cardiomyocyte Preparation
- 07:50, 10 October 2014 diff hist +6 Cardiomyocyte Preparation
- 07:46, 10 October 2014 diff hist -10 Modified myotube prep
- 07:43, 10 October 2014 diff hist -56 Modified myotube prep
- 07:37, 10 October 2014 diff hist 0 Modified myotube prep →Execution
- 07:36, 10 October 2014 diff hist +5 Modified myotube prep
- 07:34, 10 October 2014 diff hist -38 Modified myotube prep
- 07:29, 10 October 2014 diff hist -1 Modified myotube prep
- 07:10, 10 October 2014 diff hist +7 Modified myotube prep
- 07:05, 10 October 2014 diff hist +3,246 N Modified myotube prep Created page with "Induce A1 cells on 10 cm plates to form myotubes by * wash 1x with 5 mls APBS * changing the media to 0.5% serum-AMEM * wait 4 days If desired, myotubes can be purified awa..."
- 08:09, 26 September 2014 diff hist +4 Passaging A1 cell
- 08:07, 26 September 2014 diff hist +6 Passaging A1 cell
- 08:06, 26 September 2014 diff hist +787 Passaging A1 cell
- 07:44, 24 September 2014 diff hist +4 Thawing frozen A1 cells
- 07:43, 24 September 2014 diff hist 0 Thawing frozen A1 cells
- 07:37, 24 September 2014 diff hist +828 N Thawing frozen A1 cells Created page with "== Materials: == - Gelatin (warmed to 37ºC) - HS for A1 cells - 1x 15 ml FALCON tubes - Pipettes - 1x 175 cm2 flasks - frozen cells into liquid nitrogen == Procedure: == - p..."
- 08:45, 5 September 2014 diff hist +2 Electroporation of A1 cells
- 08:45, 5 September 2014 diff hist +15 Electroporation of A1 cells
- 08:43, 5 September 2014 diff hist +11 Electroporation of A1 cells
- 08:41, 5 September 2014 diff hist +14 Electroporation of A1 cells
- 08:36, 5 September 2014 diff hist 0 Electroporation of A1 cells
- 08:35, 5 September 2014 diff hist +7 Electroporation of A1 cells
- 08:16, 5 September 2014 diff hist -3 Electroporation of A1 cells
- 08:14, 5 September 2014 diff hist -4 Electroporation of A1 cells
- 08:12, 5 September 2014 diff hist +100 Electroporation of A1 cells
- 07:26, 5 September 2014 diff hist +3 Electroporation of A1 cells
- 07:24, 5 September 2014 diff hist 0 Electroporation of A1 cells
- 07:23, 5 September 2014 diff hist -5 Electroporation of A1 cells
- 07:22, 5 September 2014 diff hist -13 Electroporation of A1 cells
- 07:20, 5 September 2014 diff hist +12 Electroporation of A1 cells
- 07:19, 5 September 2014 diff hist +4 Electroporation of A1 cells
- 07:18, 5 September 2014 diff hist +899 N Electroporation of A1 cells Created page with "==Materials:== Ice 4 mM cuvettes, N+1 cuvettes Steinberg's, at 4 C. Square pulse electroporator (we use the BTX 830 Squarporator) 1) Put cuvettes on ice, and cool down..."
- 09:33, 3 September 2014 diff hist 0 Thawing frozen C2C12 cells
- 09:31, 3 September 2014 diff hist +711 N Thawing frozen C2C12 cells Created page with "==Materials:== - frozen C2C12 cells - HS for C2C12 cells - pipettes and tips - FALCON tube 15 ml - Cell culture flask ==Procedure:== - heat the HS up to 37ºC - pipette 14 ml..."
- 09:09, 3 September 2014 diff hist 0 Myotube preparation of C2C12 cells
- 09:05, 3 September 2014 diff hist 0 Myotube preparation of C2C12 cells
- 09:03, 3 September 2014 diff hist +8 Myotube preparation of C2C12 cells
- 09:02, 3 September 2014 diff hist +2,773 N Myotube preparation of C2C12 cells Created page with "Materials for 1 x 96 well plate: - DMEM (3.5 ml) –purely - Fibronectin (final diluted concentration should be 20 ng/µl)) - 1x 96 well plate - 2% Horse Serum – Low Serum (..."
- 07:17, 29 August 2014 diff hist -6 Whole mount ISH on axolotl tissue
- 07:15, 29 August 2014 diff hist -24 Whole mount ISH on axolotl tissue
- 07:14, 29 August 2014 diff hist +1 Whole mount ISH on axolotl tissue
- 07:14, 29 August 2014 diff hist +3 Whole mount ISH on axolotl tissue
- 07:13, 29 August 2014 diff hist +1 Whole mount ISH on axolotl tissue
- 07:12, 29 August 2014 diff hist +19 Whole mount ISH on axolotl tissue
- 07:08, 29 August 2014 diff hist +3 Whole mount ISH on axolotl tissue
- 07:06, 29 August 2014 diff hist +6 Whole mount ISH on axolotl tissue
- 07:04, 29 August 2014 diff hist -40 Whole mount ISH on axolotl tissue
- 07:00, 29 August 2014 diff hist +3,046 N Whole mount ISH on axolotl tissue Created page with "# fix embryo or tail blastema in MEMFA (0.1M MOPS pH7.4, 2mM EGTA, 1mM MgSO4, 3.7% formaldehyde) 2h to over night at room temperature # store at –20C in 100% MeOH or EtOH (p..."
- 10:28, 22 August 2014 diff hist +835 N Labelling Pax6 with Dig-NHS Created page with "2 June 2005 Pax6 AB is at 5 mg/ml, 1 ml 1) Dialyze 500 µl into PBS O/N then one change in morning 2 hours. 2) Spin antibody and check protein concentration by BioRad STD ..."
- 08:02, 22 August 2014 diff hist +22 Thawing 293FT cells
- 07:56, 22 August 2014 diff hist -9 Thawing 293FT cells
- 07:52, 22 August 2014 diff hist +846 N Thawing 293FT cells Created page with "→ thaw the cells in prewarmed , complete medium without Geneticin materials: - a vial of frozen cells from the liquid nitrogen - 37ºC waterbath - 75 cm2 flask - HS for 293..."
- 09:43, 15 August 2014 diff hist +6 G418 medium preparation
- 09:41, 15 August 2014 diff hist +4 G418 medium preparation
- 09:39, 15 August 2014 diff hist +9 G418 medium preparation
- 09:37, 15 August 2014 diff hist +6 G418 medium preparation
- 09:35, 15 August 2014 diff hist +10 G418 medium preparation
- 09:33, 15 August 2014 diff hist +6 G418 medium preparation
- 09:30, 15 August 2014 diff hist +878 N G418 medium preparation Created page with "'''Materials:''' - 2x 15 ml FALCON tube - filter 0.22 µl - 5 ml syringe - DMEM - G418 '''Execution:''' 1 g of weight has a total concentration from 0,751 g How much of DMEM ..."
- 08:01, 11 August 2014 diff hist -11 Cardiomyocyte Preparation
- 08:00, 11 August 2014 diff hist +15 Cardiomyocyte Preparation
- 08:00, 11 August 2014 diff hist -4 Cardiomyocyte Preparation
- 07:59, 11 August 2014 diff hist 0 Cardiomyocyte Preparation
- 07:57, 11 August 2014 diff hist 0 Cardiomyocyte Preparation
- 07:55, 11 August 2014 diff hist -15 Cardiomyocyte Preparation
- 07:40, 11 August 2014 diff hist +1,449 N Cardiomyocyte Preparation Created page with "Bettencourt-Dias & Laube F combined 1) Collect hearts in 70% Leibovitz-15 (L15) medium (AL15) w/ pen-strep 2) Wash them once in 70% Leibovitz-15 (L15) medium (AL15) w/ pen-st..."
- 07:55, 6 August 2014 diff hist -1,895 Passaging A1 cell Blanked the page
- 07:53, 6 August 2014 diff hist +1,895 N A1 cell passaging myotube Created page with "<h4>For passaging 3 flasks of A1 cells:</h4> <li>1 flask into 3 flasks and <li>2 flasks into 2 x 10 cm plates (for making myotubes) <h3>Materials :</h3> <li>HS AMEM (see add..."