Serum immunostaining

Responsible person

If you have any troubles please contact Dunja.

Protocol

The following protocol applies to Gibco Goat Serum from Invitrogen (Ordernr.: 16210).

1. Defrost the serum (takes a long time- maybe overnight in the fridge is practical)
2. Heat inactivate for 30 min at 56C
3. Cool on ice and aliquot into 50ml Falcon tubes.
4. Keep at -20 C

For detailed information see "Heat Inactivation of Serum".