Difference between revisions of "Serum immunostaining"
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| − | For detailed information see [[Heat_Inactivation_of_Serum.pdf]]. | + | For detailed information see the [[Media:Heat_Inactivation_of_Serum.pdf|Detailed protocol]]. |
Revision as of 15:54, 8 November 2012
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Protocol
The following protocol applies to Gibco Goat Serum from Invitrogen (Order No.: 16210).
- Defrost the serum (takes a long time- maybe overnight in the fridge is practical)
- Heat inactivate for 30 min at 56C
- Cool on ice and aliquot into 50ml Falcon tubes.
- Keep at -20 C
For detailed information see the Detailed protocol.