Difference between revisions of "Serum immunostaining"
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(Created page with "== Responsible person == If you have any troubles please contact Dunja. == Protocol == The following protocol applies to '''Gibco Goat Serum''' from Invitrogen (Ordernr.: 16...") |
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<li>Cool on ice and aliquot into 50ml Falcon tubes.</li> | <li>Cool on ice and aliquot into 50ml Falcon tubes.</li> | ||
<li>Keep at -20 C</li> | <li>Keep at -20 C</li> | ||
| − | </ | + | </ol> |
For detailed information see Heat Inactivation of Serum. | For detailed information see Heat Inactivation of Serum. | ||
Revision as of 15:50, 7 November 2012
Responsible person
If you have any troubles please contact Dunja.
Protocol
The following protocol applies to Gibco Goat Serum from Invitrogen (Ordernr.: 16210).
- Defrost the serum (takes a long time- maybe overnight in the fridge is practical)
- Heat inactivate for 30 min at 56C
- Cool on ice and aliquot into 50ml Falcon tubes.
- Keep at -20 C
For detailed information see Heat Inactivation of Serum.