RNA formaldehyde gels

Prepare gel box:

Clean gel box and combs etc with detergent, then dionized water.

Then incubate the gel box and components in 10% H2O2 (hydrogen peroxide, stored in cold room) for 30 minutes.

Rinse box thoroughly with DEPC water.

For making gel and preparing samples:

Your should pour (and ideally run) the gel in the hood, since the gel contains formaldehyde. [see protocol from Maniatis (original edition)—attached.]