Difference between revisions of "Purifying IgG1 from Hybridoma Supernatant"
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Supernatant (100-200 ml)
Peristaltic pump
1 ml HiTrap ProteinG column (binding capacity = 25 ml)
PBS (100 ml)
10x PBS
.1 M glycine pH 2.7 (25 ml)
1 M Tris pH 9.0 (10 ml)
10 Eppendorf tubes
20% ETOH/H2O (25 ml)
(Created page with "<h3>Materials:</h3> <li>Supernatant (100-200 ml)</li> <li>Peristaltic pump</li> <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml)</li> <li>PBS (100 ml)</li> <li>10x ...") |
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| Line 1: | Line 1: | ||
<h3>Materials:</h3> | <h3>Materials:</h3> | ||
| − | <li>Supernatant (100-200 ml) | + | <li>Supernatant (100-200 ml) |
| − | <li>Peristaltic pump | + | <li>Peristaltic pump |
| − | <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml) | + | <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml) |
| − | <li>PBS (100 ml) | + | <li>PBS (100 ml) |
| − | <li>10x PBS | + | <li>10x PBS |
| − | <li>.1 M glycine pH 2.7 (25 ml) | + | <li>.1 M glycine pH 2.7 (25 ml) |
| − | <li>1 M Tris pH 9.0 (10 ml) | + | <li>1 M Tris pH 9.0 (10 ml) |
| − | <li>10 Eppendorf tubes | + | <li>10 Eppendorf tubes |
| − | <li>20% ETOH/H2O (25 ml) | + | <li>20% ETOH/H2O (25 ml) |