Difference between revisions of "Purifying IgG1 from Hybridoma Supernatant"
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Supernatant (100-200 ml)
Peristaltic pump
1 ml HiTrap ProteinG column (binding capacity = 25 ml)
PBS (100 ml)
10x PBS
.1 M glycine pH 2.7 (25 ml)
1 M Tris pH 9.0 (10 ml)
10 Eppendorf tubes
20% ETOH/H2O (25 ml)
(Created page with "<h3>Materials:</h3> <li>Supernatant (100-200 ml)</li> <li>Peristaltic pump</li> <li>1 ml HiTrap ProteinG column (binding capacity = 25 ml)</li> <li>PBS (100 ml)</li> <li>10x ...") |
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